Experiment: LB_plus_SM_buffer with Br60_phage 0.000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3637 and GFF3638 are separated by 59 nucleotides GFF3638 and GFF3639 are separated by 58 nucleotides GFF3639 and GFF3640 are separated by 104 nucleotides
GFF3637 - Tyrosyl-tRNA synthetase (EC 6.1.1.1), at 385,540 to 386,814
GFF3637
GFF3638 - Pyridoxal kinase (EC 2.7.1.35), at 386,874 to 387,734
GFF3638
GFF3639 - Glutathione S-transferase (EC 2.5.1.18), at 387,793 to 388,398
GFF3639
GFF3640 - Di/tripeptide permease DtpA, at 388,503 to 390,008
GFF3640
Position (kb) 387
388
389 Strain fitness (log2 ratio) -1
0
1 at 386.820 kb on + strand at 386.821 kb on - strand at 386.821 kb on - strand at 386.821 kb on - strand at 387.009 kb on + strand, within GFF3638 at 387.107 kb on - strand, within GFF3638 at 387.237 kb on - strand, within GFF3638 at 387.488 kb on + strand, within GFF3638 at 387.489 kb on - strand, within GFF3638 at 387.489 kb on - strand, within GFF3638 at 387.491 kb on + strand, within GFF3638 at 387.711 kb on + strand at 387.737 kb on - strand at 387.795 kb on - strand at 387.795 kb on - strand at 387.795 kb on - strand at 387.929 kb on + strand, within GFF3639 at 388.098 kb on + strand, within GFF3639 at 388.101 kb on - strand, within GFF3639 at 388.137 kb on + strand, within GFF3639 at 388.137 kb on + strand, within GFF3639 at 388.192 kb on - strand, within GFF3639 at 388.428 kb on + strand at 388.433 kb on + strand at 388.630 kb on - strand at 388.703 kb on - strand, within GFF3640 at 388.830 kb on - strand, within GFF3640 at 388.941 kb on - strand, within GFF3640 at 388.991 kb on - strand, within GFF3640 at 388.991 kb on - strand, within GFF3640 at 389.189 kb on + strand, within GFF3640 at 389.271 kb on - strand, within GFF3640 at 389.327 kb on + strand, within GFF3640
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.000005 MOI remove 386,820 + +1.2 386,821 - +0.9 386,821 - -0.5 386,821 - +0.5 387,009 + GFF3638 0.16 -0.6 387,107 - GFF3638 0.27 -0.4 387,237 - GFF3638 0.42 +0.3 387,488 + GFF3638 0.71 -0.3 387,489 - GFF3638 0.71 +0.6 387,489 - GFF3638 0.71 +0.5 387,491 + GFF3638 0.72 +0.4 387,711 + -0.4 387,737 - -1.2 387,795 - +0.8 387,795 - +0.3 387,795 - +0.3 387,929 + GFF3639 0.22 -0.1 388,098 + GFF3639 0.50 +0.3 388,101 - GFF3639 0.51 +0.1 388,137 + GFF3639 0.57 +1.4 388,137 + GFF3639 0.57 +0.1 388,192 - GFF3639 0.66 +0.8 388,428 + -0.0 388,433 + -0.3 388,630 - +0.2 388,703 - GFF3640 0.13 -0.3 388,830 - GFF3640 0.22 +0.8 388,941 - GFF3640 0.29 +0.0 388,991 - GFF3640 0.32 +0.6 388,991 - GFF3640 0.32 +0.5 389,189 + GFF3640 0.46 -0.2 389,271 - GFF3640 0.51 +0.4 389,327 + GFF3640 0.55 -0.8
Or see this region's nucleotide sequence