Experiment: LB_plus_SM_buffer with Br60_phage 0.000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3249 and GFF3250 are separated by 216 nucleotides GFF3250 and GFF3251 overlap by 14 nucleotides
GFF3249 - Cell division inhibitor, at 24,240 to 24,749
GFF3249
GFF3250 - DNA transformation protein TfoX, at 24,966 to 25,571
GFF3250
GFF3251 - Putative efflux (PET) family inner membrane protein YccS, at 25,558 to 27,711
GFF3251
Position (kb) 24
25
26 Strain fitness (log2 ratio) -1
0
1 at 23.988 kb on - strand at 23.988 kb on - strand at 23.992 kb on - strand at 24.022 kb on - strand at 24.036 kb on - strand at 24.048 kb on - strand at 24.093 kb on + strand at 24.121 kb on - strand at 24.181 kb on + strand at 24.339 kb on + strand, within GFF3249 at 24.405 kb on + strand, within GFF3249 at 24.406 kb on - strand, within GFF3249 at 24.416 kb on - strand, within GFF3249 at 24.426 kb on - strand, within GFF3249 at 24.580 kb on - strand, within GFF3249 at 24.746 kb on - strand at 24.749 kb on + strand at 24.750 kb on - strand at 24.750 kb on - strand at 25.207 kb on - strand, within GFF3250 at 25.209 kb on + strand, within GFF3250 at 25.266 kb on + strand, within GFF3250 at 25.309 kb on + strand, within GFF3250 at 25.337 kb on + strand, within GFF3250 at 25.392 kb on - strand, within GFF3250 at 25.392 kb on - strand, within GFF3250 at 25.471 kb on - strand, within GFF3250 at 25.499 kb on - strand, within GFF3250 at 25.573 kb on + strand at 25.574 kb on - strand at 25.574 kb on - strand at 25.855 kb on - strand, within GFF3251 at 25.856 kb on + strand, within GFF3251 at 25.863 kb on + strand, within GFF3251 at 25.939 kb on - strand, within GFF3251 at 25.941 kb on + strand, within GFF3251 at 25.941 kb on + strand, within GFF3251 at 26.064 kb on + strand, within GFF3251 at 26.124 kb on + strand, within GFF3251 at 26.125 kb on - strand, within GFF3251 at 26.162 kb on - strand, within GFF3251 at 26.315 kb on - strand, within GFF3251 at 26.420 kb on + strand, within GFF3251 at 26.421 kb on - strand, within GFF3251 at 26.421 kb on - strand, within GFF3251 at 26.421 kb on - strand, within GFF3251 at 26.424 kb on + strand, within GFF3251 at 26.425 kb on - strand, within GFF3251
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.000005 MOI remove 23,988 - -0.2 23,988 - +0.3 23,992 - +0.4 24,022 - +0.2 24,036 - +0.1 24,048 - +0.3 24,093 + +0.3 24,121 - +0.1 24,181 + +0.0 24,339 + GFF3249 0.19 -0.8 24,405 + GFF3249 0.32 +0.4 24,406 - GFF3249 0.33 -1.2 24,416 - GFF3249 0.35 +0.4 24,426 - GFF3249 0.36 -0.1 24,580 - GFF3249 0.67 -1.0 24,746 - +0.2 24,749 + +0.6 24,750 - +1.2 24,750 - +0.5 25,207 - GFF3250 0.40 +0.1 25,209 + GFF3250 0.40 +0.5 25,266 + GFF3250 0.50 +0.3 25,309 + GFF3250 0.57 -0.7 25,337 + GFF3250 0.61 -1.3 25,392 - GFF3250 0.70 +0.2 25,392 - GFF3250 0.70 -0.2 25,471 - GFF3250 0.83 +0.3 25,499 - GFF3250 0.88 -1.4 25,573 + +1.2 25,574 - -0.5 25,574 - +0.3 25,855 - GFF3251 0.14 -0.3 25,856 + GFF3251 0.14 +0.3 25,863 + GFF3251 0.14 -0.4 25,939 - GFF3251 0.18 -0.4 25,941 + GFF3251 0.18 -0.1 25,941 + GFF3251 0.18 +0.6 26,064 + GFF3251 0.23 +0.4 26,124 + GFF3251 0.26 +0.5 26,125 - GFF3251 0.26 -1.0 26,162 - GFF3251 0.28 +0.5 26,315 - GFF3251 0.35 -0.1 26,420 + GFF3251 0.40 +0.5 26,421 - GFF3251 0.40 -0.1 26,421 - GFF3251 0.40 +0.3 26,421 - GFF3251 0.40 +1.4 26,424 + GFF3251 0.40 -1.5 26,425 - GFF3251 0.40 -1.4
Or see this region's nucleotide sequence