Experiment: LB_plus_SM_buffer with Br60_phage 0.000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3162 and GFF3163 are separated by 96 nucleotides GFF3163 and GFF3164 are separated by 11 nucleotides GFF3164 and GFF3165 are separated by 18 nucleotides GFF3165 and GFF3166 are separated by 459 nucleotides
GFF3162 - glycogen synthesis protein GlgS, at 164,449 to 164,658
GFF3162
GFF3163 - hypothetical protein, at 164,755 to 164,904
GFF3163
GFF3164 - Putative inner membrane protein, at 164,916 to 165,536
GFF3164
GFF3165 - Inner membrane protein YqiK, at 165,555 to 167,234
GFF3165
GFF3166 - ADP-heptose synthase (EC 2.7.-.-) / D-glycero-beta-D-manno-heptose 7-phosphate kinase, at 167,694 to 169,127
GFF3166
Position (kb) 165
166
167
168 Strain fitness (log2 ratio) -3
-2
-1
0
1 at 164.623 kb on - strand, within GFF3162 at 164.688 kb on + strand at 164.725 kb on - strand at 164.741 kb on + strand at 164.741 kb on + strand at 164.741 kb on + strand at 164.797 kb on + strand, within GFF3163 at 164.797 kb on + strand, within GFF3163 at 164.908 kb on + strand at 164.936 kb on + strand at 164.937 kb on - strand at 165.052 kb on - strand, within GFF3164 at 165.094 kb on - strand, within GFF3164 at 165.405 kb on - strand, within GFF3164 at 165.427 kb on + strand, within GFF3164 at 165.427 kb on + strand, within GFF3164 at 165.427 kb on + strand, within GFF3164 at 165.428 kb on - strand, within GFF3164 at 165.521 kb on + strand at 165.522 kb on - strand at 165.528 kb on + strand at 165.529 kb on - strand at 165.534 kb on + strand at 165.535 kb on - strand at 165.554 kb on - strand at 165.554 kb on - strand at 165.575 kb on - strand at 165.623 kb on - strand at 165.773 kb on + strand, within GFF3165 at 165.773 kb on + strand, within GFF3165 at 165.778 kb on + strand, within GFF3165 at 165.779 kb on - strand, within GFF3165 at 165.863 kb on - strand, within GFF3165 at 165.863 kb on - strand, within GFF3165 at 165.863 kb on - strand, within GFF3165 at 165.868 kb on + strand, within GFF3165 at 166.214 kb on + strand, within GFF3165 at 166.308 kb on - strand, within GFF3165 at 166.499 kb on - strand, within GFF3165 at 166.506 kb on - strand, within GFF3165 at 166.506 kb on - strand, within GFF3165 at 166.556 kb on + strand, within GFF3165 at 166.912 kb on + strand, within GFF3165 at 166.921 kb on + strand, within GFF3165 at 167.095 kb on - strand at 167.095 kb on - strand at 167.245 kb on + strand at 167.246 kb on - strand at 167.509 kb on - strand at 167.573 kb on - strand at 167.695 kb on + strand at 167.940 kb on - strand, within GFF3166 at 167.987 kb on + strand, within GFF3166
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.000005 MOI remove 164,623 - GFF3162 0.83 -0.5 164,688 + -0.2 164,725 - +0.0 164,741 + -1.2 164,741 + -0.2 164,741 + -0.4 164,797 + GFF3163 0.28 -0.1 164,797 + GFF3163 0.28 -0.1 164,908 + -0.2 164,936 + -0.5 164,937 - -0.5 165,052 - GFF3164 0.22 -0.1 165,094 - GFF3164 0.29 +0.2 165,405 - GFF3164 0.79 -0.6 165,427 + GFF3164 0.82 +0.0 165,427 + GFF3164 0.82 +0.3 165,427 + GFF3164 0.82 +0.1 165,428 - GFF3164 0.82 +0.5 165,521 + +0.2 165,522 - +0.5 165,528 + +0.7 165,529 - -0.7 165,534 + -0.6 165,535 - +0.6 165,554 - -0.8 165,554 - +0.3 165,575 - -0.5 165,623 - +0.1 165,773 + GFF3165 0.13 +0.3 165,773 + GFF3165 0.13 +0.0 165,778 + GFF3165 0.13 -0.4 165,779 - GFF3165 0.13 +0.5 165,863 - GFF3165 0.18 -0.6 165,863 - GFF3165 0.18 -1.1 165,863 - GFF3165 0.18 -0.5 165,868 + GFF3165 0.19 +0.3 166,214 + GFF3165 0.39 +1.0 166,308 - GFF3165 0.45 -0.1 166,499 - GFF3165 0.56 -1.9 166,506 - GFF3165 0.57 -0.0 166,506 - GFF3165 0.57 -0.8 166,556 + GFF3165 0.60 +0.1 166,912 + GFF3165 0.81 +0.1 166,921 + GFF3165 0.81 +0.2 167,095 - -0.6 167,095 - -0.3 167,245 + +0.2 167,246 - +0.3 167,509 - +0.8 167,573 - -0.1 167,695 + -0.3 167,940 - GFF3166 0.17 -3.4 167,987 + GFF3166 0.20 -2.3
Or see this region's nucleotide sequence