Experiment: LB_plus_SM_buffer with Br60_phage 0.000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF293 and GFF294 are separated by 17 nucleotides GFF294 and GFF295 are separated by 225 nucleotides
GFF293 - D-serine dehydratase (EC 4.3.1.18), at 7,303 to 8,625
GFF293
GFF294 - D-serine permease DsdX, at 8,643 to 9,980
GFF294
GFF295 - D-serine dehydratase transcriptional activator, at 10,206 to 11,129
GFF295
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-1
0
1
2 at 7.681 kb on - strand, within GFF293 at 7.960 kb on - strand, within GFF293 at 7.997 kb on + strand, within GFF293 at 8.003 kb on - strand, within GFF293 at 8.064 kb on + strand, within GFF293 at 8.064 kb on + strand, within GFF293 at 8.064 kb on + strand, within GFF293 at 8.064 kb on + strand, within GFF293 at 8.068 kb on + strand, within GFF293 at 8.068 kb on + strand, within GFF293 at 8.069 kb on - strand, within GFF293 at 8.069 kb on - strand, within GFF293 at 8.080 kb on - strand, within GFF293 at 8.104 kb on - strand, within GFF293 at 8.193 kb on + strand, within GFF293 at 8.193 kb on + strand, within GFF293 at 8.194 kb on - strand, within GFF293 at 8.196 kb on - strand, within GFF293 at 8.261 kb on + strand, within GFF293 at 8.261 kb on + strand, within GFF293 at 8.262 kb on - strand, within GFF293 at 8.486 kb on - strand, within GFF293 at 8.511 kb on - strand at 8.628 kb on + strand at 8.629 kb on - strand at 8.638 kb on + strand at 8.638 kb on + strand at 8.652 kb on - strand at 8.963 kb on + strand, within GFF294 at 8.963 kb on + strand, within GFF294 at 8.963 kb on + strand, within GFF294 at 8.963 kb on + strand, within GFF294 at 8.964 kb on - strand, within GFF294 at 8.964 kb on - strand, within GFF294 at 8.964 kb on - strand, within GFF294 at 8.964 kb on - strand, within GFF294 at 8.970 kb on - strand, within GFF294 at 8.970 kb on - strand, within GFF294 at 8.970 kb on - strand, within GFF294 at 9.095 kb on + strand, within GFF294 at 9.099 kb on + strand, within GFF294 at 9.100 kb on - strand, within GFF294 at 9.100 kb on - strand, within GFF294 at 9.100 kb on - strand, within GFF294 at 9.100 kb on - strand, within GFF294 at 9.116 kb on + strand, within GFF294 at 9.135 kb on + strand, within GFF294 at 9.136 kb on - strand, within GFF294 at 9.140 kb on - strand, within GFF294 at 9.140 kb on - strand, within GFF294 at 9.140 kb on - strand, within GFF294 at 9.203 kb on + strand, within GFF294 at 9.205 kb on + strand, within GFF294 at 9.205 kb on + strand, within GFF294 at 9.387 kb on + strand, within GFF294 at 9.432 kb on + strand, within GFF294 at 9.433 kb on - strand, within GFF294 at 9.439 kb on + strand, within GFF294 at 9.581 kb on + strand, within GFF294 at 9.924 kb on + strand at 9.925 kb on - strand at 9.981 kb on - strand at 9.990 kb on - strand at 10.237 kb on + strand at 10.266 kb on + strand at 10.268 kb on + strand at 10.353 kb on - strand, within GFF295 at 10.527 kb on + strand, within GFF295 at 10.581 kb on - strand, within GFF295 at 10.629 kb on - strand, within GFF295 at 10.664 kb on + strand, within GFF295 at 10.668 kb on + strand, within GFF295 at 10.668 kb on + strand, within GFF295 at 10.668 kb on + strand, within GFF295 at 10.669 kb on - strand, within GFF295 at 10.807 kb on + strand, within GFF295 at 10.830 kb on - strand, within GFF295
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.000005 MOI remove 7,681 - GFF293 0.29 -1.3 7,960 - GFF293 0.50 +1.2 7,997 + GFF293 0.52 -0.3 8,003 - GFF293 0.53 +0.2 8,064 + GFF293 0.58 +0.6 8,064 + GFF293 0.58 -0.2 8,064 + GFF293 0.58 -0.0 8,064 + GFF293 0.58 -0.9 8,068 + GFF293 0.58 +0.6 8,068 + GFF293 0.58 -0.1 8,069 - GFF293 0.58 -0.0 8,069 - GFF293 0.58 +0.0 8,080 - GFF293 0.59 -0.1 8,104 - GFF293 0.61 +0.1 8,193 + GFF293 0.67 +0.0 8,193 + GFF293 0.67 -1.5 8,194 - GFF293 0.67 +0.1 8,196 - GFF293 0.67 -0.2 8,261 + GFF293 0.72 +1.0 8,261 + GFF293 0.72 +0.1 8,262 - GFF293 0.72 -0.1 8,486 - GFF293 0.89 -0.1 8,511 - +0.2 8,628 + -0.0 8,629 - -0.2 8,638 + -0.4 8,638 + -0.2 8,652 - +0.6 8,963 + GFF294 0.24 -0.5 8,963 + GFF294 0.24 -1.0 8,963 + GFF294 0.24 -0.1 8,963 + GFF294 0.24 +0.2 8,964 - GFF294 0.24 -0.6 8,964 - GFF294 0.24 -0.3 8,964 - GFF294 0.24 -0.5 8,964 - GFF294 0.24 -0.3 8,970 - GFF294 0.24 -0.4 8,970 - GFF294 0.24 -0.0 8,970 - GFF294 0.24 -1.3 9,095 + GFF294 0.34 -0.6 9,099 + GFF294 0.34 -0.4 9,100 - GFF294 0.34 -1.4 9,100 - GFF294 0.34 +0.2 9,100 - GFF294 0.34 +2.4 9,100 - GFF294 0.34 +0.1 9,116 + GFF294 0.35 -0.3 9,135 + GFF294 0.37 +0.3 9,136 - GFF294 0.37 -0.0 9,140 - GFF294 0.37 +0.2 9,140 - GFF294 0.37 -0.4 9,140 - GFF294 0.37 -0.4 9,203 + GFF294 0.42 -0.7 9,205 + GFF294 0.42 +0.0 9,205 + GFF294 0.42 -0.0 9,387 + GFF294 0.56 -0.0 9,432 + GFF294 0.59 +0.6 9,433 - GFF294 0.59 -0.5 9,439 + GFF294 0.59 -0.2 9,581 + GFF294 0.70 +1.3 9,924 + -1.4 9,925 - +0.1 9,981 - +1.1 9,990 - -0.9 10,237 + -0.3 10,266 + +0.3 10,268 + -0.1 10,353 - GFF295 0.16 -0.6 10,527 + GFF295 0.35 -0.1 10,581 - GFF295 0.41 +0.1 10,629 - GFF295 0.46 +0.4 10,664 + GFF295 0.50 +1.2 10,668 + GFF295 0.50 +0.1 10,668 + GFF295 0.50 -0.3 10,668 + GFF295 0.50 +0.1 10,669 - GFF295 0.50 -0.3 10,807 + GFF295 0.65 -0.1 10,830 - GFF295 0.68 +0.9
Or see this region's nucleotide sequence