Experiment: LB_plus_SM_buffer with Br60_phage 0.000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2097 and GFF2098 are separated by 72 nucleotides GFF2098 and GFF2099 are separated by 203 nucleotides GFF2099 and GFF2100 are separated by 115 nucleotides
GFF2097 - hypothetical protein, at 6,858 to 7,538
GFF2097
GFF2098 - Thioredoxin 2 (EC 1.8.1.8), at 7,611 to 8,030
GFF2098
GFF2099 - hypothetical tRNA/rRNA methyltransferase yfiF [EC:2.1.1.-], at 8,234 to 9,271
GFF2099
GFF2100 - Uracil-DNA glycosylase, family 1, at 9,387 to 10,076
GFF2100
Position (kb)
8
9
10 Strain fitness (log2 ratio)
-2
-1
0
1 at 7.406 kb on + strand, within GFF2097 at 7.530 kb on - strand at 7.670 kb on + strand, within GFF2098 at 7.826 kb on + strand, within GFF2098 at 8.030 kb on + strand at 8.030 kb on + strand at 8.031 kb on - strand at 8.031 kb on - strand at 8.031 kb on - strand at 8.279 kb on + strand at 8.279 kb on + strand at 8.279 kb on + strand at 8.420 kb on - strand, within GFF2099 at 8.420 kb on - strand, within GFF2099 at 8.515 kb on + strand, within GFF2099 at 8.515 kb on + strand, within GFF2099 at 8.782 kb on + strand, within GFF2099 at 8.803 kb on - strand, within GFF2099 at 8.882 kb on - strand, within GFF2099 at 8.917 kb on - strand, within GFF2099 at 9.120 kb on + strand, within GFF2099 at 9.137 kb on + strand, within GFF2099 at 9.314 kb on + strand at 9.315 kb on - strand at 9.533 kb on + strand, within GFF2100 at 9.533 kb on + strand, within GFF2100 at 9.799 kb on + strand, within GFF2100 at 9.800 kb on - strand, within GFF2100 at 9.804 kb on - strand, within GFF2100 at 9.804 kb on - strand, within GFF2100 at 9.804 kb on - strand, within GFF2100 at 9.804 kb on - strand, within GFF2100 at 10.010 kb on + strand at 10.106 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.000005 MOI remove 7,406 + GFF2097 0.80 +0.0 7,530 - +0.1 7,670 + GFF2098 0.14 -0.0 7,826 + GFF2098 0.51 -0.0 8,030 + -0.4 8,030 + -0.1 8,031 - +0.5 8,031 - +0.2 8,031 - +0.8 8,279 + -0.5 8,279 + +0.1 8,279 + +0.6 8,420 - GFF2099 0.18 -1.1 8,420 - GFF2099 0.18 -1.8 8,515 + GFF2099 0.27 +0.3 8,515 + GFF2099 0.27 -0.2 8,782 + GFF2099 0.53 +0.4 8,803 - GFF2099 0.55 +0.1 8,882 - GFF2099 0.62 +0.1 8,917 - GFF2099 0.66 -0.5 9,120 + GFF2099 0.85 +0.4 9,137 + GFF2099 0.87 +0.1 9,314 + +0.1 9,315 - +0.4 9,533 + GFF2100 0.21 +0.0 9,533 + GFF2100 0.21 -2.5 9,799 + GFF2100 0.60 -0.1 9,800 - GFF2100 0.60 +0.4 9,804 - GFF2100 0.60 -0.6 9,804 - GFF2100 0.60 -0.5 9,804 - GFF2100 0.60 +0.2 9,804 - GFF2100 0.60 -0.9 10,010 + -0.8 10,106 + -0.4
Or see this region's nucleotide sequence