Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1298

Experiment: LB_plus_SM_buffer with Br60_phage 0.000005 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1298 and GFF1299 are separated by 232 nucleotides GFF1298 - N-acetylmuramoyl-L-alanine amidase (EC 3.5.1.28), at 164 to 1,417 GFF1298 GFF1299 - N-acetylglutamate synthase (EC 2.3.1.1), at 1,650 to 2,981 GFF1299 Position (kb) 0 1 2Strain fitness (log2 ratio) -3 -2 -1 0 1at 0.058 kb on + strandat 0.128 kb on + strandat 0.128 kb on + strandat 0.165 kb on + strandat 0.202 kb on - strandat 0.290 kb on - strand, within GFF1298at 0.452 kb on - strand, within GFF1298at 0.474 kb on + strand, within GFF1298at 0.475 kb on - strand, within GFF1298at 0.507 kb on - strand, within GFF1298at 0.628 kb on + strand, within GFF1298at 0.713 kb on - strand, within GFF1298at 0.715 kb on - strand, within GFF1298at 0.715 kb on - strand, within GFF1298at 0.936 kb on + strand, within GFF1298at 1.043 kb on + strand, within GFF1298at 1.128 kb on + strand, within GFF1298at 1.166 kb on - strand, within GFF1298at 1.199 kb on + strand, within GFF1298at 1.200 kb on - strand, within GFF1298at 1.200 kb on - strand, within GFF1298at 1.238 kb on - strand, within GFF1298at 1.239 kb on + strand, within GFF1298at 1.262 kb on + strand, within GFF1298at 1.349 kb on + strandat 1.353 kb on - strandat 1.376 kb on + strandat 1.376 kb on + strandat 1.451 kb on - strandat 1.509 kb on + strandat 1.530 kb on + strandat 1.530 kb on + strandat 1.532 kb on + strandat 1.707 kb on - strandat 1.740 kb on - strandat 1.809 kb on - strand, within GFF1299at 1.974 kb on - strand, within GFF1299at 2.028 kb on - strand, within GFF1299at 2.376 kb on + strand, within GFF1299

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.000005 MOI
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58 + +0.3
128 + +0.2
128 + -0.6
165 + -0.0
202 - -0.6
290 - GFF1298 0.10 -0.1
452 - GFF1298 0.23 +0.3
474 + GFF1298 0.25 +0.3
475 - GFF1298 0.25 +1.4
507 - GFF1298 0.27 -0.6
628 + GFF1298 0.37 +0.9
713 - GFF1298 0.44 +0.3
715 - GFF1298 0.44 -0.3
715 - GFF1298 0.44 +0.2
936 + GFF1298 0.62 +0.1
1,043 + GFF1298 0.70 +1.0
1,128 + GFF1298 0.77 +0.4
1,166 - GFF1298 0.80 -0.6
1,199 + GFF1298 0.83 +0.2
1,200 - GFF1298 0.83 +0.9
1,200 - GFF1298 0.83 -0.1
1,238 - GFF1298 0.86 -0.3
1,239 + GFF1298 0.86 +0.3
1,262 + GFF1298 0.88 +0.1
1,349 + +0.1
1,353 - +0.1
1,376 + +0.3
1,376 + +0.3
1,451 - -0.3
1,509 + +0.1
1,530 + +0.2
1,530 + -0.4
1,532 + -0.5
1,707 - -0.4
1,740 - +0.1
1,809 - GFF1299 0.12 -0.0
1,974 - GFF1299 0.24 -3.2
2,028 - GFF1299 0.28 +0.1
2,376 + GFF1299 0.55 +0.3

Or see this region's nucleotide sequence