Experiment: LB_plus_SM_buffer with Br60_phage 0.000005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1119 and GFF1120 are separated by 61 nucleotides GFF1120 and GFF1121 are separated by 54 nucleotides
GFF1119 - Na+/H+ antiporter NhaA type, at 306,974 to 308,140
GFF1119
GFF1120 - Transcriptional activator NhaR, at 308,202 to 309,101
GFF1120
GFF1121 - Alpha-xylosidase (EC 3.2.1.-), at 309,156 to 311,195
GFF1121
Position (kb) 308
309
310 Strain fitness (log2 ratio) -2
-1
0
1
2 at 307.595 kb on + strand, within GFF1119 at 307.597 kb on + strand, within GFF1119 at 307.597 kb on + strand, within GFF1119 at 307.598 kb on - strand, within GFF1119 at 307.598 kb on - strand, within GFF1119 at 307.598 kb on - strand, within GFF1119 at 307.946 kb on + strand, within GFF1119 at 307.946 kb on + strand, within GFF1119 at 307.947 kb on - strand, within GFF1119 at 308.045 kb on - strand at 308.105 kb on - strand at 308.217 kb on - strand at 308.223 kb on + strand at 308.223 kb on + strand at 308.236 kb on - strand at 308.253 kb on + strand at 308.253 kb on + strand at 308.366 kb on + strand, within GFF1120 at 308.367 kb on - strand, within GFF1120 at 308.432 kb on + strand, within GFF1120 at 308.573 kb on - strand, within GFF1120 at 308.645 kb on + strand, within GFF1120 at 308.887 kb on + strand, within GFF1120 at 309.009 kb on + strand, within GFF1120 at 309.009 kb on + strand, within GFF1120 at 309.090 kb on - strand at 309.099 kb on + strand at 309.100 kb on - strand at 309.100 kb on - strand at 309.106 kb on - strand at 309.178 kb on + strand at 309.197 kb on + strand at 309.229 kb on + strand at 309.253 kb on + strand at 309.321 kb on - strand at 309.525 kb on + strand, within GFF1121 at 309.526 kb on - strand, within GFF1121 at 309.707 kb on + strand, within GFF1121 at 309.750 kb on + strand, within GFF1121 at 309.803 kb on + strand, within GFF1121 at 309.803 kb on + strand, within GFF1121 at 309.818 kb on + strand, within GFF1121 at 309.934 kb on + strand, within GFF1121 at 309.934 kb on + strand, within GFF1121 at 309.938 kb on + strand, within GFF1121 at 309.938 kb on + strand, within GFF1121 at 309.938 kb on + strand, within GFF1121 at 309.938 kb on + strand, within GFF1121 at 309.938 kb on + strand, within GFF1121 at 309.938 kb on + strand, within GFF1121 at 309.939 kb on - strand, within GFF1121 at 309.946 kb on + strand, within GFF1121 at 309.950 kb on + strand, within GFF1121 at 309.951 kb on - strand, within GFF1121 at 309.951 kb on - strand, within GFF1121 at 309.997 kb on + strand, within GFF1121 at 310.003 kb on + strand, within GFF1121 at 310.033 kb on - strand, within GFF1121 at 310.064 kb on - strand, within GFF1121
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.000005 MOI remove 307,595 + GFF1119 0.53 +1.0 307,597 + GFF1119 0.53 +0.5 307,597 + GFF1119 0.53 +0.8 307,598 - GFF1119 0.53 +1.1 307,598 - GFF1119 0.53 -0.5 307,598 - GFF1119 0.53 -0.0 307,946 + GFF1119 0.83 +0.1 307,946 + GFF1119 0.83 -2.3 307,947 - GFF1119 0.83 +0.3 308,045 - +1.3 308,105 - -0.9 308,217 - +0.1 308,223 + +0.7 308,223 + +0.1 308,236 - +0.3 308,253 + +2.7 308,253 + +0.1 308,366 + GFF1120 0.18 +0.1 308,367 - GFF1120 0.18 +0.6 308,432 + GFF1120 0.26 +0.7 308,573 - GFF1120 0.41 +0.4 308,645 + GFF1120 0.49 +0.7 308,887 + GFF1120 0.76 +0.6 309,009 + GFF1120 0.90 +0.3 309,009 + GFF1120 0.90 +0.8 309,090 - +0.2 309,099 + +0.3 309,100 - -1.4 309,100 - +0.1 309,106 - +0.2 309,178 + +0.5 309,197 + -0.3 309,229 + -0.5 309,253 + +0.6 309,321 - +0.2 309,525 + GFF1121 0.18 -0.2 309,526 - GFF1121 0.18 +1.0 309,707 + GFF1121 0.27 +0.6 309,750 + GFF1121 0.29 +0.1 309,803 + GFF1121 0.32 -1.3 309,803 + GFF1121 0.32 +1.2 309,818 + GFF1121 0.32 +0.3 309,934 + GFF1121 0.38 +0.2 309,934 + GFF1121 0.38 +0.3 309,938 + GFF1121 0.38 -0.7 309,938 + GFF1121 0.38 -0.5 309,938 + GFF1121 0.38 +0.1 309,938 + GFF1121 0.38 +0.1 309,938 + GFF1121 0.38 +0.2 309,938 + GFF1121 0.38 +0.3 309,939 - GFF1121 0.38 +0.4 309,946 + GFF1121 0.39 +0.1 309,950 + GFF1121 0.39 +0.2 309,951 - GFF1121 0.39 +2.6 309,951 - GFF1121 0.39 -0.1 309,997 + GFF1121 0.41 +0.4 310,003 + GFF1121 0.42 -0.8 310,033 - GFF1121 0.43 -0.5 310,064 - GFF1121 0.45 +0.5
Or see this region's nucleotide sequence