Experiment: R2A with Rifampicin 0.0007 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0077 and Atu0078 are separated by 174 nucleotides Atu0078 and Atu0079 overlap by 8 nucleotides Atu0079 and Atu0080 are separated by 477 nucleotides
Atu0077: Atu0077 - D-isomer specific 2-hydroxyacid dehydrogenases family protein, at 79,181 to 80,185
Atu0077
Atu0078: Atu0078 - hypothetical protein, at 80,360 to 80,899
Atu0078
Atu0079: Atu0079 - Atu0079-1 mutant of pfkB family carbohydrate kinase, at 80,892 to 81,884
Atu0079
Atu0080: Atu0080 - permease, at 82,362 to 83,306
Atu0080
Position (kb)
80
81
82 Strain fitness (log2 ratio)
-1
0
1 at 79.892 kb on - strand, within Atu0077 at 80.021 kb on - strand, within Atu0077 at 80.044 kb on + strand, within Atu0077 at 80.062 kb on + strand, within Atu0077 at 80.161 kb on - strand at 80.169 kb on + strand at 80.193 kb on - strand at 81.015 kb on - strand, within Atu0079 at 81.017 kb on + strand, within Atu0079 at 81.020 kb on - strand, within Atu0079 at 81.025 kb on - strand, within Atu0079 at 81.062 kb on - strand, within Atu0079 at 81.096 kb on - strand, within Atu0079 at 81.101 kb on - strand, within Atu0079 at 81.118 kb on - strand, within Atu0079 at 81.257 kb on + strand, within Atu0079 at 81.336 kb on - strand, within Atu0079 at 81.411 kb on - strand, within Atu0079 at 81.536 kb on + strand, within Atu0079 at 81.726 kb on - strand, within Atu0079 at 81.742 kb on + strand, within Atu0079 at 81.887 kb on + strand at 81.887 kb on + strand at 81.895 kb on - strand at 81.935 kb on - strand at 82.005 kb on - strand at 82.022 kb on + strand at 82.031 kb on - strand at 82.096 kb on - strand at 82.178 kb on + strand at 82.186 kb on - strand at 82.220 kb on + strand at 82.261 kb on - strand at 82.429 kb on + strand at 82.434 kb on + strand at 82.439 kb on + strand at 82.439 kb on + strand at 82.439 kb on + strand at 82.581 kb on + strand, within Atu0080 at 82.612 kb on + strand, within Atu0080 at 82.677 kb on + strand, within Atu0080 at 82.794 kb on - strand, within Atu0080
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Rifampicin 0.0007 mM remove 79,892 - Atu0077 0.71 -0.0 80,021 - Atu0077 0.84 -0.5 80,044 + Atu0077 0.86 +0.0 80,062 + Atu0077 0.88 -0.8 80,161 - -0.7 80,169 + -0.5 80,193 - +0.6 81,015 - Atu0079 0.12 -0.3 81,017 + Atu0079 0.13 -0.8 81,020 - Atu0079 0.13 -0.1 81,025 - Atu0079 0.13 -0.0 81,062 - Atu0079 0.17 +0.1 81,096 - Atu0079 0.21 -1.2 81,101 - Atu0079 0.21 +0.6 81,118 - Atu0079 0.23 -0.1 81,257 + Atu0079 0.37 +0.1 81,336 - Atu0079 0.45 -0.9 81,411 - Atu0079 0.52 +0.4 81,536 + Atu0079 0.65 +0.5 81,726 - Atu0079 0.84 +0.3 81,742 + Atu0079 0.86 -0.6 81,887 + +0.2 81,887 + +0.1 81,895 - +0.3 81,935 - -0.4 82,005 - +0.2 82,022 + -0.7 82,031 - -0.1 82,096 - +0.1 82,178 + -0.0 82,186 - +0.2 82,220 + +0.2 82,261 - -0.9 82,429 + -0.4 82,434 + +0.4 82,439 + -0.4 82,439 + -0.8 82,439 + +0.4 82,581 + Atu0080 0.23 +1.0 82,612 + Atu0080 0.26 -0.1 82,677 + Atu0080 0.33 +0.1 82,794 - Atu0080 0.46 -0.5
Or see this region's nucleotide sequence