Experiment: LB_plus_SM_buffer with Br60_phage 0.00005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF913 and GFF914 overlap by 4 nucleotides GFF914 and GFF915 are separated by 16 nucleotides GFF915 and GFF916 are separated by 77 nucleotides GFF916 and GFF917 overlap by 11 nucleotides GFF917 and GFF918 are separated by 3 nucleotides
GFF913 - D-Glucosaminate-6-phosphate ammonia-lyase (EC 4.3.1.-), at 90,937 to 92,055
GFF913
GFF914 - 2-dehydro-3-deoxyphosphogluconate aldolase (EC 4.1.2.14) in D-glucosaminate utilization operon, at 92,052 to 92,792
GFF914
GFF915 - Putative BglB-family transcriptional antiterminator, at 92,809 to 94,722
GFF915
GFF916 - RelB/StbD replicon stabilization protein (antitoxin to RelE/StbE), at 94,800 to 95,042
GFF916
GFF917 - RelE/StbE replicon stabilization toxin, at 95,032 to 95,316
GFF917
GFF918 - Ribonucleotide reductase of class III (anaerobic), activating protein (EC 1.97.1.4), at 95,320 to 95,784
GFF918
Position (kb) 92
93
94
95 Strain fitness (log2 ratio) -1
0
1 at 91.811 kb on + strand, within GFF913 at 91.921 kb on + strand, within GFF913 at 91.930 kb on + strand, within GFF913 at 91.931 kb on - strand, within GFF913 at 92.001 kb on - strand at 92.220 kb on + strand, within GFF914 at 92.232 kb on - strand, within GFF914 at 92.311 kb on + strand, within GFF914 at 92.482 kb on + strand, within GFF914 at 92.568 kb on + strand, within GFF914 at 92.569 kb on - strand, within GFF914 at 92.570 kb on + strand, within GFF914 at 92.571 kb on - strand, within GFF914 at 92.578 kb on + strand, within GFF914 at 92.681 kb on - strand, within GFF914 at 92.703 kb on + strand, within GFF914 at 92.706 kb on - strand, within GFF914 at 92.706 kb on - strand, within GFF914 at 92.720 kb on + strand at 92.720 kb on + strand at 92.761 kb on + strand at 92.761 kb on + strand at 93.090 kb on - strand, within GFF915 at 93.094 kb on + strand, within GFF915 at 93.095 kb on - strand, within GFF915 at 93.308 kb on + strand, within GFF915 at 93.308 kb on + strand, within GFF915 at 93.309 kb on - strand, within GFF915 at 93.351 kb on + strand, within GFF915 at 93.352 kb on - strand, within GFF915 at 93.573 kb on - strand, within GFF915 at 93.575 kb on + strand, within GFF915 at 93.614 kb on + strand, within GFF915 at 93.634 kb on - strand, within GFF915 at 93.634 kb on - strand, within GFF915 at 93.922 kb on + strand, within GFF915 at 93.923 kb on - strand, within GFF915 at 93.925 kb on - strand, within GFF915 at 93.989 kb on - strand, within GFF915 at 94.019 kb on + strand, within GFF915 at 94.119 kb on - strand, within GFF915 at 94.229 kb on - strand, within GFF915 at 94.334 kb on + strand, within GFF915 at 94.441 kb on - strand, within GFF915 at 94.849 kb on - strand, within GFF916 at 94.898 kb on - strand, within GFF916 at 95.038 kb on + strand at 95.038 kb on + strand at 95.038 kb on + strand at 95.038 kb on + strand at 95.079 kb on + strand, within GFF917 at 95.080 kb on - strand, within GFF917 at 95.132 kb on + strand, within GFF917 at 95.132 kb on + strand, within GFF917 at 95.196 kb on + strand, within GFF917 at 95.315 kb on - strand at 95.485 kb on - strand, within GFF918 at 95.519 kb on - strand, within GFF918 at 95.519 kb on - strand, within GFF918 at 95.625 kb on + strand, within GFF918 at 95.626 kb on - strand, within GFF918 at 95.643 kb on + strand, within GFF918 at 95.684 kb on + strand, within GFF918
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.00005 MOI remove 91,811 + GFF913 0.78 +0.1 91,921 + GFF913 0.88 +0.6 91,930 + GFF913 0.89 -0.5 91,931 - GFF913 0.89 -0.1 92,001 - +0.4 92,220 + GFF914 0.23 -0.5 92,232 - GFF914 0.24 +0.1 92,311 + GFF914 0.35 -0.4 92,482 + GFF914 0.58 +0.2 92,568 + GFF914 0.70 -0.3 92,569 - GFF914 0.70 +0.8 92,570 + GFF914 0.70 -0.1 92,571 - GFF914 0.70 +0.2 92,578 + GFF914 0.71 +1.0 92,681 - GFF914 0.85 -0.3 92,703 + GFF914 0.88 +0.8 92,706 - GFF914 0.88 +0.2 92,706 - GFF914 0.88 -0.1 92,720 + -0.0 92,720 + -0.2 92,761 + -0.0 92,761 + -0.5 93,090 - GFF915 0.15 -0.4 93,094 + GFF915 0.15 -0.1 93,095 - GFF915 0.15 +0.1 93,308 + GFF915 0.26 +0.0 93,308 + GFF915 0.26 -1.0 93,309 - GFF915 0.26 -0.4 93,351 + GFF915 0.28 +0.0 93,352 - GFF915 0.28 +0.3 93,573 - GFF915 0.40 -0.0 93,575 + GFF915 0.40 -0.2 93,614 + GFF915 0.42 -0.2 93,634 - GFF915 0.43 +0.5 93,634 - GFF915 0.43 +0.3 93,922 + GFF915 0.58 -0.1 93,923 - GFF915 0.58 +0.2 93,925 - GFF915 0.58 -0.3 93,989 - GFF915 0.62 -0.3 94,019 + GFF915 0.63 +0.3 94,119 - GFF915 0.68 +0.2 94,229 - GFF915 0.74 -0.1 94,334 + GFF915 0.80 -0.1 94,441 - GFF915 0.85 -0.5 94,849 - GFF916 0.20 +1.0 94,898 - GFF916 0.40 +0.6 95,038 + +0.4 95,038 + +0.2 95,038 + +0.1 95,038 + +0.1 95,079 + GFF917 0.16 +1.1 95,080 - GFF917 0.17 -0.0 95,132 + GFF917 0.35 -0.2 95,132 + GFF917 0.35 -0.2 95,196 + GFF917 0.58 +0.3 95,315 - +0.5 95,485 - GFF918 0.35 +1.2 95,519 - GFF918 0.43 -0.0 95,519 - GFF918 0.43 +0.5 95,625 + GFF918 0.66 +0.1 95,626 - GFF918 0.66 -0.3 95,643 + GFF918 0.69 +0.2 95,684 + GFF918 0.78 +0.3
Or see this region's nucleotide sequence