Experiment: LB_plus_SM_buffer with Br60_phage 0.00005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF866 and GFF867 are separated by 12 nucleotides GFF867 and GFF868 are separated by 33 nucleotides GFF868 and GFF869 overlap by 11 nucleotides GFF869 and GFF870 are separated by 326 nucleotides
GFF866 - '2',3'-cyclic-nucleotide 2'-phosphodiesterase (EC 3.1.4.16)' transl_table=11, at 42,909 to 44,852
GFF866
GFF867 - FIG01048799: hypothetical protein, at 44,865 to 45,086
GFF867
GFF868 - '3'(2'),5'-bisphosphate nucleotidase (EC 3.1.3.7)' transl_table=11, at 45,120 to 45,860
GFF868
GFF869 - Protein ytfJ precursor, at 45,850 to 46,407
GFF869
GFF870 - FIG00614015: hypothetical protein, at 46,734 to 46,940
GFF870
Position (kb) 45
46 Strain fitness (log2 ratio) -2
-1
0
1 at 44.155 kb on + strand, within GFF866 at 44.190 kb on + strand, within GFF866 at 44.191 kb on - strand, within GFF866 at 44.389 kb on + strand, within GFF866 at 44.495 kb on + strand, within GFF866 at 44.594 kb on + strand, within GFF866 at 44.594 kb on + strand, within GFF866 at 44.594 kb on + strand, within GFF866 at 44.597 kb on - strand, within GFF866 at 44.695 kb on - strand at 44.695 kb on - strand at 44.729 kb on - strand at 44.846 kb on + strand at 44.846 kb on + strand at 44.867 kb on - strand at 44.878 kb on - strand at 44.901 kb on - strand, within GFF867 at 45.023 kb on + strand, within GFF867 at 45.073 kb on + strand at 45.092 kb on + strand at 45.133 kb on + strand at 45.134 kb on - strand at 45.177 kb on + strand at 45.177 kb on + strand at 45.489 kb on + strand, within GFF868 at 45.490 kb on - strand, within GFF868 at 45.490 kb on - strand, within GFF868 at 45.507 kb on - strand, within GFF868 at 45.537 kb on - strand, within GFF868 at 45.808 kb on - strand at 45.851 kb on + strand at 45.852 kb on - strand at 45.867 kb on + strand at 46.038 kb on - strand, within GFF869 at 46.050 kb on + strand, within GFF869 at 46.058 kb on - strand, within GFF869 at 46.058 kb on - strand, within GFF869 at 46.058 kb on - strand, within GFF869 at 46.078 kb on - strand, within GFF869 at 46.128 kb on - strand, within GFF869 at 46.156 kb on - strand, within GFF869 at 46.360 kb on + strand at 46.396 kb on - strand at 46.459 kb on - strand at 46.468 kb on + strand at 46.469 kb on - strand at 46.549 kb on - strand at 46.550 kb on + strand at 46.551 kb on - strand at 46.595 kb on - strand at 46.610 kb on - strand at 46.611 kb on + strand at 46.611 kb on + strand at 46.611 kb on + strand at 46.636 kb on - strand at 46.639 kb on + strand at 46.640 kb on - strand at 46.646 kb on + strand at 46.646 kb on + strand at 46.647 kb on - strand at 46.648 kb on + strand at 46.716 kb on - strand at 46.716 kb on - strand at 46.716 kb on - strand at 46.716 kb on - strand at 46.752 kb on + strand at 46.752 kb on + strand at 46.805 kb on - strand, within GFF870 at 46.806 kb on + strand, within GFF870 at 46.806 kb on + strand, within GFF870 at 46.806 kb on + strand, within GFF870 at 46.806 kb on + strand, within GFF870 at 46.807 kb on - strand, within GFF870 at 46.807 kb on - strand, within GFF870 at 46.807 kb on - strand, within GFF870
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.00005 MOI remove 44,155 + GFF866 0.64 +0.1 44,190 + GFF866 0.66 -0.2 44,191 - GFF866 0.66 -0.3 44,389 + GFF866 0.76 +0.1 44,495 + GFF866 0.82 +0.0 44,594 + GFF866 0.87 -0.1 44,594 + GFF866 0.87 +0.5 44,594 + GFF866 0.87 +0.1 44,597 - GFF866 0.87 +1.5 44,695 - -0.3 44,695 - +0.9 44,729 - +0.2 44,846 + -1.3 44,846 + -0.1 44,867 - -0.3 44,878 - -0.1 44,901 - GFF867 0.16 -0.6 45,023 + GFF867 0.71 -0.4 45,073 + -0.1 45,092 + +0.0 45,133 + -0.4 45,134 - -1.3 45,177 + -1.0 45,177 + -0.4 45,489 + GFF868 0.50 -2.3 45,490 - GFF868 0.50 -1.5 45,490 - GFF868 0.50 -0.5 45,507 - GFF868 0.52 +0.1 45,537 - GFF868 0.56 -0.4 45,808 - -0.7 45,851 + -0.2 45,852 - -0.3 45,867 + +0.3 46,038 - GFF869 0.34 +0.6 46,050 + GFF869 0.36 -0.2 46,058 - GFF869 0.37 +0.1 46,058 - GFF869 0.37 -0.0 46,058 - GFF869 0.37 -0.1 46,078 - GFF869 0.41 -0.1 46,128 - GFF869 0.50 -0.2 46,156 - GFF869 0.55 +0.3 46,360 + -0.3 46,396 - +0.3 46,459 - -0.1 46,468 + -0.2 46,469 - -0.5 46,549 - -0.2 46,550 + -0.1 46,551 - +0.2 46,595 - +0.4 46,610 - +0.5 46,611 + +0.2 46,611 + +0.1 46,611 + +0.3 46,636 - +0.0 46,639 + +0.1 46,640 - +0.9 46,646 + +0.6 46,646 + +0.2 46,647 - +0.4 46,648 + +0.0 46,716 - -0.0 46,716 - +0.6 46,716 - -0.9 46,716 - -0.2 46,752 + +0.1 46,752 + +1.1 46,805 - GFF870 0.34 -0.3 46,806 + GFF870 0.35 +1.0 46,806 + GFF870 0.35 +1.7 46,806 + GFF870 0.35 -0.2 46,806 + GFF870 0.35 -0.1 46,807 - GFF870 0.35 -0.7 46,807 - GFF870 0.35 -0.0 46,807 - GFF870 0.35 +0.4
Or see this region's nucleotide sequence