Experiment: LB_plus_SM_buffer with Br60_phage 0.00005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF707 and GFF708 are separated by 115 nucleotides GFF708 and GFF709 are separated by 28 nucleotides GFF709 and GFF710 are separated by 353 nucleotides GFF710 and GFF711 are separated by 62 nucleotides
GFF707 - Non-specific DNA-binding protein Dps / Iron-binding ferritin-like antioxidant protein / Ferroxidase (EC 1.16.3.1), at 64,551 to 65,054
GFF707
GFF708 - FIG01046955: hypothetical protein, at 65,170 to 65,328
GFF708
GFF709 - Putative DMT superfamily metabolite efflux protein precursor, at 65,357 to 66,244
GFF709
GFF710 - Outer membrane protein X precursor, at 66,598 to 67,113
GFF710
GFF711 - FIG00732228: membrane protein, at 67,176 to 68,756
GFF711
Position (kb) 65
66
67 Strain fitness (log2 ratio) -1
0
1
2 at 64.365 kb on + strand at 64.428 kb on + strand at 64.429 kb on - strand at 64.429 kb on - strand at 64.429 kb on - strand at 64.429 kb on - strand at 64.430 kb on + strand at 64.430 kb on + strand at 64.431 kb on - strand at 64.433 kb on - strand at 64.440 kb on + strand at 64.440 kb on + strand at 64.440 kb on + strand at 64.531 kb on + strand at 64.540 kb on + strand at 64.541 kb on - strand at 64.547 kb on - strand at 64.718 kb on + strand, within GFF707 at 64.718 kb on + strand, within GFF707 at 64.878 kb on - strand, within GFF707 at 65.035 kb on - strand at 65.062 kb on + strand at 65.408 kb on - strand at 65.578 kb on - strand, within GFF709 at 65.757 kb on + strand, within GFF709 at 65.821 kb on + strand, within GFF709 at 65.821 kb on + strand, within GFF709 at 65.822 kb on - strand, within GFF709 at 65.822 kb on - strand, within GFF709 at 65.822 kb on - strand, within GFF709 at 65.822 kb on - strand, within GFF709 at 65.822 kb on - strand, within GFF709 at 65.878 kb on + strand, within GFF709 at 65.979 kb on + strand, within GFF709 at 65.980 kb on - strand, within GFF709 at 65.984 kb on + strand, within GFF709 at 65.984 kb on + strand, within GFF709 at 65.984 kb on + strand, within GFF709 at 65.985 kb on - strand, within GFF709 at 65.988 kb on + strand, within GFF709 at 66.015 kb on + strand, within GFF709 at 66.015 kb on + strand, within GFF709 at 66.015 kb on + strand, within GFF709 at 66.016 kb on - strand, within GFF709 at 66.226 kb on + strand at 66.395 kb on - strand at 66.429 kb on - strand at 66.439 kb on + strand at 66.495 kb on + strand at 66.573 kb on + strand at 66.573 kb on + strand at 66.662 kb on + strand, within GFF710 at 66.675 kb on + strand, within GFF710 at 66.675 kb on + strand, within GFF710 at 66.748 kb on + strand, within GFF710 at 66.749 kb on - strand, within GFF710 at 66.836 kb on - strand, within GFF710 at 66.904 kb on + strand, within GFF710 at 66.979 kb on + strand, within GFF710 at 66.992 kb on - strand, within GFF710 at 67.065 kb on + strand at 67.111 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.00005 MOI remove 64,365 + +0.3 64,428 + +0.4 64,429 - +0.2 64,429 - -0.2 64,429 - +0.9 64,429 - +0.1 64,430 + -0.2 64,430 + -0.3 64,431 - -0.2 64,433 - -0.4 64,440 + -0.3 64,440 + +0.1 64,440 + +0.1 64,531 + +0.4 64,540 + -1.0 64,541 - -0.2 64,547 - +0.4 64,718 + GFF707 0.33 +0.3 64,718 + GFF707 0.33 -0.2 64,878 - GFF707 0.65 +1.2 65,035 - +0.3 65,062 + -0.1 65,408 - -0.0 65,578 - GFF709 0.25 +0.3 65,757 + GFF709 0.45 +0.3 65,821 + GFF709 0.52 +0.4 65,821 + GFF709 0.52 +0.2 65,822 - GFF709 0.52 -0.6 65,822 - GFF709 0.52 +0.9 65,822 - GFF709 0.52 -0.1 65,822 - GFF709 0.52 +0.0 65,822 - GFF709 0.52 +0.0 65,878 + GFF709 0.59 +0.2 65,979 + GFF709 0.70 +0.1 65,980 - GFF709 0.70 +1.9 65,984 + GFF709 0.71 +0.3 65,984 + GFF709 0.71 +0.5 65,984 + GFF709 0.71 +0.2 65,985 - GFF709 0.71 +0.6 65,988 + GFF709 0.71 -0.0 66,015 + GFF709 0.74 +0.2 66,015 + GFF709 0.74 -0.2 66,015 + GFF709 0.74 -0.5 66,016 - GFF709 0.74 +0.4 66,226 + +0.0 66,395 - +0.2 66,429 - -0.3 66,439 + -0.0 66,495 + +0.4 66,573 + +0.1 66,573 + +0.3 66,662 + GFF710 0.12 -0.5 66,675 + GFF710 0.15 +0.9 66,675 + GFF710 0.15 +0.1 66,748 + GFF710 0.29 +0.2 66,749 - GFF710 0.29 -1.6 66,836 - GFF710 0.46 +1.8 66,904 + GFF710 0.59 +0.1 66,979 + GFF710 0.74 +0.1 66,992 - GFF710 0.76 +0.8 67,065 + -0.2 67,111 + +0.3
Or see this region's nucleotide sequence