Experiment: LB_plus_SM_buffer with Br60_phage 0.00005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF666 and GFF667 overlap by 1 nucleotides GFF667 and GFF668 are separated by 1 nucleotides GFF668 and GFF669 are separated by 84 nucleotides GFF669 and GFF670 are separated by 57 nucleotides
GFF666 - Urocanate hydratase (EC 4.2.1.49), at 22,033 to 23,178
GFF666
GFF667 - Urocanate hydratase (EC 4.2.1.49), at 23,178 to 23,717
GFF667
GFF668 - Histidine ammonia-lyase (EC 4.3.1.3), at 23,719 to 25,239
GFF668
GFF669 - UPF0098 protein ybhB, at 25,324 to 25,800
GFF669
GFF670 - Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62), at 25,858 to 27,147
GFF670
Position (kb)
23
24
25
26 Strain fitness (log2 ratio)
-1
0
1 at 22.841 kb on - strand, within GFF666 at 22.848 kb on + strand, within GFF666 at 22.849 kb on - strand, within GFF666 at 22.853 kb on - strand, within GFF666 at 22.996 kb on - strand, within GFF666 at 23.073 kb on + strand at 23.073 kb on + strand at 23.144 kb on + strand at 23.144 kb on + strand at 23.177 kb on - strand at 23.177 kb on - strand at 23.189 kb on + strand at 23.267 kb on - strand, within GFF667 at 23.594 kb on - strand, within GFF667 at 23.719 kb on - strand at 23.751 kb on - strand at 23.751 kb on - strand at 23.772 kb on + strand at 24.363 kb on - strand, within GFF668 at 24.544 kb on - strand, within GFF668 at 24.585 kb on - strand, within GFF668 at 24.810 kb on + strand, within GFF668 at 24.810 kb on + strand, within GFF668 at 24.811 kb on - strand, within GFF668 at 25.146 kb on + strand at 25.147 kb on - strand at 25.233 kb on + strand at 25.233 kb on + strand at 25.234 kb on - strand at 25.234 kb on - strand at 25.446 kb on + strand, within GFF669 at 25.452 kb on + strand, within GFF669 at 25.455 kb on - strand, within GFF669 at 25.455 kb on - strand, within GFF669 at 25.714 kb on - strand, within GFF669 at 25.792 kb on - strand at 25.850 kb on - strand at 25.960 kb on + strand at 25.961 kb on - strand at 26.218 kb on - strand, within GFF670 at 26.235 kb on + strand, within GFF670 at 26.236 kb on - strand, within GFF670
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.00005 MOI remove 22,841 - GFF666 0.71 +0.2 22,848 + GFF666 0.71 -0.2 22,849 - GFF666 0.71 +0.3 22,853 - GFF666 0.72 -0.4 22,996 - GFF666 0.84 +0.9 23,073 + -0.4 23,073 + +0.4 23,144 + +0.2 23,144 + +0.1 23,177 - +0.5 23,177 - +0.1 23,189 + +0.2 23,267 - GFF667 0.16 -0.6 23,594 - GFF667 0.77 -0.3 23,719 - -0.3 23,751 - +0.1 23,751 - +0.4 23,772 + -0.1 24,363 - GFF668 0.42 +0.3 24,544 - GFF668 0.54 -0.5 24,585 - GFF668 0.57 -0.1 24,810 + GFF668 0.72 +0.0 24,810 + GFF668 0.72 +0.3 24,811 - GFF668 0.72 -0.5 25,146 + -0.4 25,147 - -0.0 25,233 + +0.1 25,233 + -0.0 25,234 - +0.6 25,234 - +0.1 25,446 + GFF669 0.26 +0.9 25,452 + GFF669 0.27 -0.1 25,455 - GFF669 0.27 +0.6 25,455 - GFF669 0.27 +0.3 25,714 - GFF669 0.82 +0.3 25,792 - -0.0 25,850 - -0.7 25,960 + +0.4 25,961 - -0.2 26,218 - GFF670 0.28 +1.3 26,235 + GFF670 0.29 +0.2 26,236 - GFF670 0.29 -0.8
Or see this region's nucleotide sequence