Experiment: LB_plus_SM_buffer with Br60_phage 0.00005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF651 and GFF652 are separated by 10 nucleotides GFF652 and GFF653 are separated by 222 nucleotides
GFF651 - Galactose-1-phosphate uridylyltransferase (EC 2.7.7.10), at 6,588 to 7,634
GFF651
GFF652 - UDP-N-acetylglucosamine 4-epimerase (EC 5.1.3.7) / UDP-glucose 4-epimerase (EC 5.1.3.2), at 7,645 to 8,661
GFF652
GFF653 - Putative inner membrane protein, at 8,884 to 9,792
GFF653
Position (kb)
7
8
9 Strain fitness (log2 ratio)
-6
-5
-4
-3
-2
-1
0
1 at 6.689 kb on - strand at 6.903 kb on - strand, within GFF651 at 7.033 kb on + strand, within GFF651 at 7.417 kb on + strand, within GFF651 at 7.418 kb on - strand, within GFF651 at 7.592 kb on + strand at 7.594 kb on + strand at 7.623 kb on - strand at 7.634 kb on + strand at 7.805 kb on + strand, within GFF652 at 7.831 kb on - strand, within GFF652 at 7.884 kb on + strand, within GFF652 at 7.886 kb on + strand, within GFF652 at 7.887 kb on - strand, within GFF652 at 7.890 kb on + strand, within GFF652 at 7.890 kb on + strand, within GFF652 at 8.053 kb on - strand, within GFF652 at 8.054 kb on + strand, within GFF652 at 8.054 kb on + strand, within GFF652 at 8.055 kb on - strand, within GFF652 at 8.206 kb on - strand, within GFF652 at 8.298 kb on + strand, within GFF652 at 8.313 kb on + strand, within GFF652 at 8.680 kb on + strand at 8.681 kb on - strand at 8.686 kb on - strand at 8.693 kb on - strand at 8.698 kb on + strand at 8.985 kb on + strand, within GFF653 at 8.986 kb on - strand, within GFF653 at 9.041 kb on + strand, within GFF653 at 9.042 kb on - strand, within GFF653 at 9.203 kb on + strand, within GFF653 at 9.212 kb on + strand, within GFF653 at 9.303 kb on + strand, within GFF653 at 9.439 kb on + strand, within GFF653 at 9.597 kb on + strand, within GFF653 at 9.617 kb on - strand, within GFF653
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.00005 MOI remove 6,689 - -0.7 6,903 - GFF651 0.30 -0.4 7,033 + GFF651 0.43 +1.2 7,417 + GFF651 0.79 -0.1 7,418 - GFF651 0.79 -2.9 7,592 + -0.3 7,594 + +0.1 7,623 - -0.8 7,634 + +0.3 7,805 + GFF652 0.16 -3.2 7,831 - GFF652 0.18 -1.9 7,884 + GFF652 0.24 -5.7 7,886 + GFF652 0.24 -5.5 7,887 - GFF652 0.24 -2.8 7,890 + GFF652 0.24 -3.6 7,890 + GFF652 0.24 -2.1 8,053 - GFF652 0.40 -0.7 8,054 + GFF652 0.40 -6.3 8,054 + GFF652 0.40 -4.3 8,055 - GFF652 0.40 -2.4 8,206 - GFF652 0.55 -3.5 8,298 + GFF652 0.64 -2.0 8,313 + GFF652 0.66 -5.1 8,680 + -1.5 8,681 - -1.0 8,686 - -0.7 8,693 - +0.3 8,698 + -1.1 8,985 + GFF653 0.11 +0.1 8,986 - GFF653 0.11 -0.0 9,041 + GFF653 0.17 +1.3 9,042 - GFF653 0.17 -0.2 9,203 + GFF653 0.35 +0.0 9,212 + GFF653 0.36 +0.2 9,303 + GFF653 0.46 -0.1 9,439 + GFF653 0.61 -0.8 9,597 + GFF653 0.78 +0.4 9,617 - GFF653 0.81 -1.3
Or see this region's nucleotide sequence