Experiment: LB_plus_SM_buffer with Br60_phage 0.00005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4633 and GFF4634 are separated by 136 nucleotides GFF4634 and GFF4635 are separated by 856 nucleotides
GFF4633 - Putative transport protein, at 425,315 to 426,361
GFF4633
GFF4634 - Citrate synthase (si) (EC 2.3.3.1), at 426,498 to 427,781
GFF4634
GFF4635 - Succinate dehydrogenase cytochrome b-556 subunit, at 428,638 to 428,925
GFF4635
Position (kb)
426
427
428 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 425.514 kb on + strand, within GFF4633 at 425.515 kb on - strand, within GFF4633 at 425.645 kb on + strand, within GFF4633 at 425.655 kb on + strand, within GFF4633 at 425.655 kb on + strand, within GFF4633 at 425.813 kb on + strand, within GFF4633 at 425.814 kb on - strand, within GFF4633 at 425.927 kb on + strand, within GFF4633 at 425.928 kb on - strand, within GFF4633 at 425.931 kb on + strand, within GFF4633 at 426.172 kb on + strand, within GFF4633 at 426.230 kb on - strand, within GFF4633 at 426.400 kb on - strand at 426.400 kb on - strand at 426.544 kb on + strand at 426.544 kb on + strand at 426.697 kb on - strand, within GFF4634 at 426.697 kb on - strand, within GFF4634 at 427.066 kb on - strand, within GFF4634 at 427.115 kb on + strand, within GFF4634 at 427.134 kb on + strand, within GFF4634 at 427.135 kb on - strand, within GFF4634 at 427.247 kb on - strand, within GFF4634 at 427.520 kb on - strand, within GFF4634 at 427.720 kb on - strand at 427.881 kb on + strand at 427.911 kb on - strand at 428.016 kb on + strand at 428.033 kb on + strand at 428.043 kb on - strand at 428.051 kb on + strand at 428.084 kb on + strand at 428.085 kb on - strand at 428.085 kb on - strand at 428.140 kb on - strand at 428.265 kb on + strand at 428.265 kb on + strand at 428.325 kb on - strand at 428.354 kb on + strand at 428.389 kb on - strand at 428.663 kb on + strand at 428.666 kb on + strand at 428.666 kb on + strand at 428.667 kb on - strand, within GFF4635
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.00005 MOI remove 425,514 + GFF4633 0.19 -0.3 425,515 - GFF4633 0.19 +0.0 425,645 + GFF4633 0.32 -0.6 425,655 + GFF4633 0.32 -0.1 425,655 + GFF4633 0.32 +0.1 425,813 + GFF4633 0.48 +0.3 425,814 - GFF4633 0.48 -0.3 425,927 + GFF4633 0.58 -0.5 425,928 - GFF4633 0.59 -0.1 425,931 + GFF4633 0.59 -1.0 426,172 + GFF4633 0.82 +0.5 426,230 - GFF4633 0.87 +0.1 426,400 - -0.1 426,400 - -0.1 426,544 + -2.9 426,544 + -1.8 426,697 - GFF4634 0.15 -1.5 426,697 - GFF4634 0.15 -2.1 427,066 - GFF4634 0.44 -1.2 427,115 + GFF4634 0.48 -1.3 427,134 + GFF4634 0.50 +0.2 427,135 - GFF4634 0.50 -1.3 427,247 - GFF4634 0.58 -0.3 427,520 - GFF4634 0.80 -0.9 427,720 - -2.0 427,881 + -1.4 427,911 - -1.1 428,016 + -2.2 428,033 + -1.6 428,043 - -0.0 428,051 + -0.9 428,084 + +1.3 428,085 - -0.3 428,085 - -0.4 428,140 - +0.3 428,265 + -0.7 428,265 + -0.8 428,325 - -1.6 428,354 + -1.1 428,389 - -1.1 428,663 + -1.5 428,666 + -1.6 428,666 + -1.8 428,667 - GFF4635 0.10 -2.0
Or see this region's nucleotide sequence