Experiment: LB_plus_SM_buffer with Br60_phage 0.00005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4011 and GFF4012 are separated by 4 nucleotides GFF4012 and GFF4013 are separated by 104 nucleotides GFF4013 and GFF4014 overlap by 4 nucleotides GFF4014 and GFF4015 are separated by 21 nucleotides
GFF4011 - Flagellar motor switch protein FliM, at 280,314 to 281,318
GFF4011
GFF4012 - Flagellar biosynthesis protein FliL, at 281,323 to 281,790
GFF4012
GFF4013 - Flagellar hook-length control protein FliK, at 281,895 to 283,112
GFF4013
GFF4014 - Flagellar protein FliJ, at 283,109 to 283,552
GFF4014
GFF4015 - Flagellum-specific ATP synthase FliI, at 283,574 to 284,944
GFF4015
Position (kb) 281
282
283
284 Strain fitness (log2 ratio) -1
0
1
2 at 281.068 kb on - strand, within GFF4011 at 281.068 kb on - strand, within GFF4011 at 281.148 kb on - strand, within GFF4011 at 281.151 kb on - strand, within GFF4011 at 281.196 kb on - strand, within GFF4011 at 281.206 kb on + strand, within GFF4011 at 281.206 kb on + strand, within GFF4011 at 281.207 kb on - strand, within GFF4011 at 281.306 kb on + strand at 281.344 kb on - strand at 281.511 kb on - strand, within GFF4012 at 281.567 kb on + strand, within GFF4012 at 281.567 kb on + strand, within GFF4012 at 281.568 kb on - strand, within GFF4012 at 281.691 kb on - strand, within GFF4012 at 281.691 kb on - strand, within GFF4012 at 281.695 kb on - strand, within GFF4012 at 281.822 kb on - strand at 281.884 kb on + strand at 281.965 kb on - strand at 282.159 kb on - strand, within GFF4013 at 282.160 kb on - strand, within GFF4013 at 282.270 kb on + strand, within GFF4013 at 282.270 kb on + strand, within GFF4013 at 282.271 kb on - strand, within GFF4013 at 282.271 kb on - strand, within GFF4013 at 282.557 kb on + strand, within GFF4013 at 282.557 kb on + strand, within GFF4013 at 282.557 kb on + strand, within GFF4013 at 282.557 kb on + strand, within GFF4013 at 282.557 kb on + strand, within GFF4013 at 282.558 kb on - strand, within GFF4013 at 282.558 kb on - strand, within GFF4013 at 282.558 kb on - strand, within GFF4013 at 282.558 kb on - strand, within GFF4013 at 282.558 kb on - strand, within GFF4013 at 282.752 kb on + strand, within GFF4013 at 282.916 kb on - strand, within GFF4013 at 282.928 kb on - strand, within GFF4013 at 283.500 kb on - strand, within GFF4014 at 283.679 kb on - strand at 283.771 kb on + strand, within GFF4015 at 283.791 kb on + strand, within GFF4015
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.00005 MOI remove 281,068 - GFF4011 0.75 +0.4 281,068 - GFF4011 0.75 +1.2 281,148 - GFF4011 0.83 +0.7 281,151 - GFF4011 0.83 +0.6 281,196 - GFF4011 0.88 +1.0 281,206 + GFF4011 0.89 +0.9 281,206 + GFF4011 0.89 +1.1 281,207 - GFF4011 0.89 +1.5 281,306 + +0.6 281,344 - +0.8 281,511 - GFF4012 0.40 +0.4 281,567 + GFF4012 0.52 +0.8 281,567 + GFF4012 0.52 +0.9 281,568 - GFF4012 0.52 +1.0 281,691 - GFF4012 0.79 +0.7 281,691 - GFF4012 0.79 +1.3 281,695 - GFF4012 0.79 +1.0 281,822 - +1.6 281,884 + +2.0 281,965 - +0.8 282,159 - GFF4013 0.22 +1.2 282,160 - GFF4013 0.22 +0.7 282,270 + GFF4013 0.31 +1.9 282,270 + GFF4013 0.31 -0.1 282,271 - GFF4013 0.31 +0.8 282,271 - GFF4013 0.31 +1.0 282,557 + GFF4013 0.54 +0.7 282,557 + GFF4013 0.54 +1.0 282,557 + GFF4013 0.54 +0.1 282,557 + GFF4013 0.54 +0.3 282,557 + GFF4013 0.54 +0.5 282,558 - GFF4013 0.54 +0.5 282,558 - GFF4013 0.54 +1.1 282,558 - GFF4013 0.54 +0.8 282,558 - GFF4013 0.54 +1.2 282,558 - GFF4013 0.54 +0.6 282,752 + GFF4013 0.70 +1.1 282,916 - GFF4013 0.84 +0.2 282,928 - GFF4013 0.85 +1.2 283,500 - GFF4014 0.88 +0.0 283,679 - +0.5 283,771 + GFF4015 0.14 +1.1 283,791 + GFF4015 0.16 +1.3
Or see this region's nucleotide sequence