Experiment: LB_plus_SM_buffer with Br60_phage 0.00005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3663 and GFF3664 are separated by 185 nucleotides GFF3664 and GFF3665 are separated by 70 nucleotides
GFF3663 - Putative inner membrane protein, at 12,197 to 12,889
GFF3663
GFF3664 - Phosphoserine aminotransferase (EC 2.6.1.52), at 13,075 to 14,163
GFF3664
GFF3665 - 5-Enolpyruvylshikimate-3-phosphate synthase (EC 2.5.1.19), at 14,234 to 15,517
GFF3665
Position (kb) 13
14
15 Strain fitness (log2 ratio) -2
-1
0
1 at 12.106 kb on + strand at 12.106 kb on + strand at 12.120 kb on + strand at 12.152 kb on - strand at 12.271 kb on + strand, within GFF3663 at 12.271 kb on + strand, within GFF3663 at 12.279 kb on - strand, within GFF3663 at 12.435 kb on + strand, within GFF3663 at 12.436 kb on - strand, within GFF3663 at 12.829 kb on - strand at 13.021 kb on + strand at 13.131 kb on + strand at 13.507 kb on + strand, within GFF3664 at 13.508 kb on - strand, within GFF3664 at 13.516 kb on + strand, within GFF3664 at 13.652 kb on - strand, within GFF3664 at 13.718 kb on + strand, within GFF3664 at 13.757 kb on - strand, within GFF3664 at 13.757 kb on - strand, within GFF3664 at 13.903 kb on + strand, within GFF3664 at 13.903 kb on + strand, within GFF3664 at 13.917 kb on + strand, within GFF3664 at 13.917 kb on + strand, within GFF3664 at 13.917 kb on + strand, within GFF3664 at 13.996 kb on - strand, within GFF3664 at 14.049 kb on - strand, within GFF3664 at 14.090 kb on - strand at 14.162 kb on - strand at 14.165 kb on - strand at 14.423 kb on + strand, within GFF3665 at 14.572 kb on - strand, within GFF3665 at 14.572 kb on - strand, within GFF3665 at 14.878 kb on - strand, within GFF3665 at 14.924 kb on + strand, within GFF3665 at 14.942 kb on + strand, within GFF3665 at 14.943 kb on - strand, within GFF3665 at 15.149 kb on - strand, within GFF3665
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.00005 MOI remove 12,106 + +0.0 12,106 + +0.4 12,120 + +0.2 12,152 - -0.1 12,271 + GFF3663 0.11 +0.7 12,271 + GFF3663 0.11 +0.4 12,279 - GFF3663 0.12 -0.7 12,435 + GFF3663 0.34 -0.4 12,436 - GFF3663 0.34 +1.2 12,829 - -0.3 13,021 + -0.4 13,131 + -0.8 13,507 + GFF3664 0.40 -0.2 13,508 - GFF3664 0.40 -0.3 13,516 + GFF3664 0.40 -0.6 13,652 - GFF3664 0.53 -2.5 13,718 + GFF3664 0.59 -0.4 13,757 - GFF3664 0.63 -1.1 13,757 - GFF3664 0.63 -1.8 13,903 + GFF3664 0.76 -0.3 13,903 + GFF3664 0.76 -1.1 13,917 + GFF3664 0.77 -1.1 13,917 + GFF3664 0.77 -0.5 13,917 + GFF3664 0.77 -0.8 13,996 - GFF3664 0.85 -2.0 14,049 - GFF3664 0.89 -1.3 14,090 - -0.9 14,162 - +0.6 14,165 - +0.3 14,423 + GFF3665 0.15 -0.7 14,572 - GFF3665 0.26 -2.0 14,572 - GFF3665 0.26 -0.3 14,878 - GFF3665 0.50 +0.2 14,924 + GFF3665 0.54 -0.6 14,942 + GFF3665 0.55 -0.7 14,943 - GFF3665 0.55 -0.9 15,149 - GFF3665 0.71 -0.9
Or see this region's nucleotide sequence