Experiment: LB_plus_SM_buffer with Br60_phage 0.00005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3106 and GFF3107 overlap by 11 nucleotides GFF3107 and GFF3108 are separated by 2 nucleotides GFF3108 and GFF3109 are separated by 7 nucleotides GFF3109 and GFF3110 overlap by 4 nucleotides GFF3110 and GFF3111 are separated by 84 nucleotides
GFF3106 - Ni/Fe-hydrogenase 2 B-type cytochrome subunit, at 115,622 to 116,800
GFF3106
GFF3107 - Fe-S-cluster-containing hydrogenase components 1, at 116,790 to 117,776
GFF3107
GFF3108 - Uptake hydrogenase small subunit precursor (EC 1.12.99.6), at 117,779 to 118,897
GFF3108
GFF3109 - FIG01046062: hypothetical protein, at 118,905 to 119,090
GFF3109
GFF3110 - FIG00638753: hypothetical protein, at 119,087 to 119,374
GFF3110
GFF3111 - Methyl-accepting chemotaxis protein I (serine chemoreceptor protein), at 119,459 to 121,102
GFF3111
Position (kb) 117
118
119 Strain fitness (log2 ratio) -1
0
1 at 116.883 kb on + strand at 116.884 kb on - strand at 116.884 kb on - strand at 116.894 kb on + strand, within GFF3107 at 116.895 kb on - strand, within GFF3107 at 117.045 kb on - strand, within GFF3107 at 117.045 kb on - strand, within GFF3107 at 117.061 kb on + strand, within GFF3107 at 117.061 kb on + strand, within GFF3107 at 117.296 kb on + strand, within GFF3107 at 117.297 kb on - strand, within GFF3107 at 117.297 kb on - strand, within GFF3107 at 117.301 kb on - strand, within GFF3107 at 117.307 kb on + strand, within GFF3107 at 117.323 kb on + strand, within GFF3107 at 117.324 kb on - strand, within GFF3107 at 117.358 kb on + strand, within GFF3107 at 117.423 kb on - strand, within GFF3107 at 117.440 kb on + strand, within GFF3107 at 117.440 kb on + strand, within GFF3107 at 117.440 kb on + strand, within GFF3107 at 117.441 kb on - strand, within GFF3107 at 117.441 kb on - strand, within GFF3107 at 117.441 kb on - strand, within GFF3107 at 117.457 kb on + strand, within GFF3107 at 117.463 kb on + strand, within GFF3107 at 117.533 kb on - strand, within GFF3107 at 117.653 kb on - strand, within GFF3107 at 117.659 kb on + strand, within GFF3107 at 117.755 kb on + strand at 117.780 kb on + strand at 117.833 kb on + strand at 117.833 kb on + strand at 117.922 kb on + strand, within GFF3108 at 118.001 kb on - strand, within GFF3108 at 118.074 kb on - strand, within GFF3108 at 118.078 kb on + strand, within GFF3108 at 118.079 kb on - strand, within GFF3108 at 118.276 kb on - strand, within GFF3108 at 118.286 kb on - strand, within GFF3108 at 118.512 kb on + strand, within GFF3108 at 118.513 kb on - strand, within GFF3108 at 118.588 kb on + strand, within GFF3108 at 118.588 kb on + strand, within GFF3108 at 118.641 kb on + strand, within GFF3108 at 118.882 kb on + strand at 118.885 kb on + strand at 118.886 kb on - strand at 118.900 kb on + strand at 118.903 kb on - strand at 118.906 kb on + strand at 118.906 kb on + strand at 119.054 kb on - strand, within GFF3109 at 119.054 kb on - strand, within GFF3109 at 119.061 kb on + strand, within GFF3109 at 119.071 kb on + strand, within GFF3109 at 119.071 kb on + strand, within GFF3109 at 119.219 kb on - strand, within GFF3110 at 119.248 kb on + strand, within GFF3110 at 119.249 kb on - strand, within GFF3110 at 119.249 kb on - strand, within GFF3110 at 119.281 kb on + strand, within GFF3110 at 119.282 kb on - strand, within GFF3110 at 119.320 kb on - strand, within GFF3110 at 119.320 kb on - strand, within GFF3110 at 119.320 kb on - strand, within GFF3110 at 119.320 kb on - strand, within GFF3110 at 119.415 kb on + strand at 119.419 kb on + strand at 119.439 kb on - strand at 119.482 kb on + strand at 119.483 kb on - strand at 119.750 kb on - strand, within GFF3111
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.00005 MOI remove 116,883 + +0.5 116,884 - +0.3 116,884 - -1.2 116,894 + GFF3107 0.11 -0.1 116,895 - GFF3107 0.11 -1.0 117,045 - GFF3107 0.26 -0.2 117,045 - GFF3107 0.26 -0.2 117,061 + GFF3107 0.27 +1.0 117,061 + GFF3107 0.27 -0.6 117,296 + GFF3107 0.51 +0.2 117,297 - GFF3107 0.51 -0.2 117,297 - GFF3107 0.51 -0.1 117,301 - GFF3107 0.52 +0.1 117,307 + GFF3107 0.52 -0.7 117,323 + GFF3107 0.54 +0.0 117,324 - GFF3107 0.54 +0.1 117,358 + GFF3107 0.58 +0.3 117,423 - GFF3107 0.64 +0.2 117,440 + GFF3107 0.66 -0.2 117,440 + GFF3107 0.66 +0.3 117,440 + GFF3107 0.66 +0.6 117,441 - GFF3107 0.66 -0.6 117,441 - GFF3107 0.66 -0.1 117,441 - GFF3107 0.66 +1.0 117,457 + GFF3107 0.68 +0.3 117,463 + GFF3107 0.68 +0.6 117,533 - GFF3107 0.75 +0.1 117,653 - GFF3107 0.87 +0.2 117,659 + GFF3107 0.88 -0.6 117,755 + -1.2 117,780 + -0.1 117,833 + +0.3 117,833 + -0.3 117,922 + GFF3108 0.13 -0.2 118,001 - GFF3108 0.20 +0.3 118,074 - GFF3108 0.26 -0.5 118,078 + GFF3108 0.27 -0.4 118,079 - GFF3108 0.27 +0.1 118,276 - GFF3108 0.44 +0.0 118,286 - GFF3108 0.45 -0.2 118,512 + GFF3108 0.66 -0.3 118,513 - GFF3108 0.66 +0.2 118,588 + GFF3108 0.72 -0.9 118,588 + GFF3108 0.72 +0.3 118,641 + GFF3108 0.77 -0.1 118,882 + +0.0 118,885 + +0.7 118,886 - -0.2 118,900 + +0.4 118,903 - -0.1 118,906 + +0.4 118,906 + +0.2 119,054 - GFF3109 0.80 -0.1 119,054 - GFF3109 0.80 +0.7 119,061 + GFF3109 0.84 +0.9 119,071 + GFF3109 0.89 -0.1 119,071 + GFF3109 0.89 -0.4 119,219 - GFF3110 0.46 -0.8 119,248 + GFF3110 0.56 -0.3 119,249 - GFF3110 0.56 +0.5 119,249 - GFF3110 0.56 -0.4 119,281 + GFF3110 0.67 +0.3 119,282 - GFF3110 0.68 +0.3 119,320 - GFF3110 0.81 +0.2 119,320 - GFF3110 0.81 -0.5 119,320 - GFF3110 0.81 +0.4 119,320 - GFF3110 0.81 +0.1 119,415 + +0.2 119,419 + +0.1 119,439 - -0.2 119,482 + +0.0 119,483 - +0.5 119,750 - GFF3111 0.18 +0.3
Or see this region's nucleotide sequence