Experiment: LB_plus_SM_buffer with Br60_phage 0.00005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3033 and GFF3034 are separated by 13 nucleotides GFF3034 and GFF3035 are separated by 2 nucleotides
GFF3033 - Malate dehydrogenase (EC 1.1.1.37), at 47,460 to 48,467
GFF3033
GFF3034 - Sorbitol dehydrogenase (EC 1.1.1.14), at 48,481 to 49,497
GFF3034
GFF3035 - D-mannonate oxidoreductase (EC 1.1.1.57), at 49,500 to 50,972
GFF3035
Position (kb)
48
49
50 Strain fitness (log2 ratio)
-1
0
1 at 47.583 kb on + strand, within GFF3033 at 47.593 kb on - strand, within GFF3033 at 47.704 kb on + strand, within GFF3033 at 47.717 kb on + strand, within GFF3033 at 47.717 kb on + strand, within GFF3033 at 47.840 kb on + strand, within GFF3033 at 47.840 kb on + strand, within GFF3033 at 47.841 kb on - strand, within GFF3033 at 47.841 kb on - strand, within GFF3033 at 47.874 kb on + strand, within GFF3033 at 48.036 kb on - strand, within GFF3033 at 48.119 kb on + strand, within GFF3033 at 48.164 kb on + strand, within GFF3033 at 48.304 kb on + strand, within GFF3033 at 48.348 kb on - strand, within GFF3033 at 48.420 kb on - strand at 48.468 kb on - strand at 48.468 kb on - strand at 48.468 kb on - strand at 48.591 kb on + strand, within GFF3034 at 48.662 kb on - strand, within GFF3034 at 48.783 kb on + strand, within GFF3034 at 48.850 kb on - strand, within GFF3034 at 48.925 kb on - strand, within GFF3034 at 49.172 kb on + strand, within GFF3034 at 49.173 kb on - strand, within GFF3034 at 49.239 kb on + strand, within GFF3034 at 49.240 kb on - strand, within GFF3034 at 49.254 kb on + strand, within GFF3034 at 49.255 kb on - strand, within GFF3034 at 49.256 kb on + strand, within GFF3034 at 49.290 kb on + strand, within GFF3034 at 49.418 kb on - strand at 49.546 kb on - strand at 49.692 kb on - strand, within GFF3035 at 49.734 kb on - strand, within GFF3035 at 49.875 kb on - strand, within GFF3035 at 49.929 kb on + strand, within GFF3035 at 49.930 kb on - strand, within GFF3035 at 49.930 kb on - strand, within GFF3035 at 49.930 kb on - strand, within GFF3035 at 49.930 kb on - strand, within GFF3035 at 49.980 kb on + strand, within GFF3035 at 49.989 kb on + strand, within GFF3035 at 50.026 kb on + strand, within GFF3035 at 50.026 kb on + strand, within GFF3035 at 50.027 kb on - strand, within GFF3035 at 50.031 kb on - strand, within GFF3035 at 50.091 kb on - strand, within GFF3035 at 50.147 kb on + strand, within GFF3035 at 50.211 kb on + strand, within GFF3035 at 50.411 kb on + strand, within GFF3035 at 50.424 kb on + strand, within GFF3035 at 50.424 kb on + strand, within GFF3035
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.00005 MOI remove 47,583 + GFF3033 0.12 +0.1 47,593 - GFF3033 0.13 +0.2 47,704 + GFF3033 0.24 +0.5 47,717 + GFF3033 0.25 +0.4 47,717 + GFF3033 0.25 +0.3 47,840 + GFF3033 0.38 -0.5 47,840 + GFF3033 0.38 +0.8 47,841 - GFF3033 0.38 +0.7 47,841 - GFF3033 0.38 +1.0 47,874 + GFF3033 0.41 -0.3 48,036 - GFF3033 0.57 +0.2 48,119 + GFF3033 0.65 -0.1 48,164 + GFF3033 0.70 -0.0 48,304 + GFF3033 0.84 +0.4 48,348 - GFF3033 0.88 +0.1 48,420 - -0.1 48,468 - -0.0 48,468 - +0.0 48,468 - -0.0 48,591 + GFF3034 0.11 +0.6 48,662 - GFF3034 0.18 +0.7 48,783 + GFF3034 0.30 +1.1 48,850 - GFF3034 0.36 +0.4 48,925 - GFF3034 0.44 +0.5 49,172 + GFF3034 0.68 +0.2 49,173 - GFF3034 0.68 +0.1 49,239 + GFF3034 0.75 -0.1 49,240 - GFF3034 0.75 -0.3 49,254 + GFF3034 0.76 +0.6 49,255 - GFF3034 0.76 -0.0 49,256 + GFF3034 0.76 +0.4 49,290 + GFF3034 0.80 +0.4 49,418 - +0.4 49,546 - +0.3 49,692 - GFF3035 0.13 +0.5 49,734 - GFF3035 0.16 +0.3 49,875 - GFF3035 0.25 -0.2 49,929 + GFF3035 0.29 -0.2 49,930 - GFF3035 0.29 +0.5 49,930 - GFF3035 0.29 +0.1 49,930 - GFF3035 0.29 -1.1 49,930 - GFF3035 0.29 +0.4 49,980 + GFF3035 0.33 -0.3 49,989 + GFF3035 0.33 +0.8 50,026 + GFF3035 0.36 -0.1 50,026 + GFF3035 0.36 +0.6 50,027 - GFF3035 0.36 +0.1 50,031 - GFF3035 0.36 +0.1 50,091 - GFF3035 0.40 +0.5 50,147 + GFF3035 0.44 -0.0 50,211 + GFF3035 0.48 -0.0 50,411 + GFF3035 0.62 +0.8 50,424 + GFF3035 0.63 +0.2 50,424 + GFF3035 0.63 -0.5
Or see this region's nucleotide sequence