Experiment: LB_plus_SM_buffer with Br60_phage 0.00005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3008 and GFF3009 overlap by 4 nucleotides GFF3009 and GFF3010 are separated by 25 nucleotides GFF3010 and GFF3011 are separated by 166 nucleotides
GFF3008 - 2-octaprenyl-6-methoxyphenol hydroxylase (EC 1.14.13.-), at 23,599 to 24,777
GFF3008
GFF3009 - Xaa-Pro aminopeptidase (EC 3.4.11.9), at 24,774 to 26,090
GFF3009
GFF3010 - UPF0149 exported protein YgfB, at 26,116 to 26,694
GFF3010
GFF3011 - Z-ring-associated protein ZapA, at 26,861 to 27,190
GFF3011
Position (kb)
24
25
26
27 Strain fitness (log2 ratio)
-2
-1
0
1 at 24.937 kb on + strand, within GFF3009 at 24.938 kb on - strand, within GFF3009 at 24.952 kb on - strand, within GFF3009 at 25.176 kb on - strand, within GFF3009 at 25.302 kb on - strand, within GFF3009 at 25.304 kb on - strand, within GFF3009 at 25.410 kb on - strand, within GFF3009 at 25.412 kb on - strand, within GFF3009 at 25.902 kb on - strand, within GFF3009 at 25.909 kb on - strand, within GFF3009 at 25.976 kb on - strand at 26.117 kb on + strand at 26.122 kb on + strand at 26.122 kb on + strand at 26.134 kb on - strand at 26.183 kb on - strand, within GFF3010 at 26.204 kb on + strand, within GFF3010 at 26.205 kb on - strand, within GFF3010 at 26.205 kb on - strand, within GFF3010 at 26.262 kb on - strand, within GFF3010 at 26.277 kb on + strand, within GFF3010 at 26.281 kb on - strand, within GFF3010 at 26.331 kb on + strand, within GFF3010 at 26.667 kb on - strand at 26.688 kb on + strand at 26.689 kb on - strand at 26.689 kb on - strand at 26.689 kb on - strand at 26.689 kb on - strand at 26.694 kb on + strand at 26.694 kb on + strand at 26.695 kb on - strand at 26.695 kb on - strand at 26.719 kb on + strand at 26.719 kb on + strand at 26.720 kb on - strand at 26.729 kb on - strand at 26.787 kb on - strand at 26.790 kb on + strand at 26.793 kb on - strand at 26.889 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.00005 MOI remove 24,937 + GFF3009 0.12 +1.3 24,938 - GFF3009 0.12 -0.6 24,952 - GFF3009 0.14 +0.2 25,176 - GFF3009 0.31 -0.3 25,302 - GFF3009 0.40 -0.9 25,304 - GFF3009 0.40 -1.0 25,410 - GFF3009 0.48 +0.0 25,412 - GFF3009 0.48 -1.5 25,902 - GFF3009 0.86 -1.2 25,909 - GFF3009 0.86 -0.1 25,976 - -2.3 26,117 + -1.0 26,122 + -0.0 26,122 + -0.7 26,134 - +0.0 26,183 - GFF3010 0.12 -0.3 26,204 + GFF3010 0.15 -1.3 26,205 - GFF3010 0.15 -0.7 26,205 - GFF3010 0.15 +0.4 26,262 - GFF3010 0.25 -0.1 26,277 + GFF3010 0.28 -0.7 26,281 - GFF3010 0.28 +0.8 26,331 + GFF3010 0.37 +0.1 26,667 - +0.2 26,688 + -0.4 26,689 - +0.2 26,689 - +0.0 26,689 - -0.2 26,689 - -0.4 26,694 + +0.4 26,694 + -0.2 26,695 - +0.1 26,695 - +0.3 26,719 + -0.5 26,719 + +1.3 26,720 - -1.0 26,729 - -1.2 26,787 - +0.1 26,790 + -1.2 26,793 - -0.6 26,889 + -0.9
Or see this region's nucleotide sequence