Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF2902

Experiment: LB_plus_SM_buffer with Br60_phage 0.00005 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF2901 and GFF2902 are separated by 160 nucleotidesGFF2902 and GFF2903 are separated by 17 nucleotidesGFF2903 and GFF2904 overlap by 4 nucleotides GFF2901 - Dihydroxy-acid dehydratase (EC 4.2.1.9), at 27,353 to 29,203 GFF2901 GFF2902 - Branched-chain amino acid aminotransferase (EC 2.6.1.42), at 29,364 to 30,293 GFF2902 GFF2903 - Acetolactate synthase small subunit (EC 2.2.1.6), at 30,311 to 30,574 GFF2903 GFF2904 - Acetolactate synthase large subunit (EC 2.2.1.6), at 30,571 to 32,217 GFF2904 Position (kb) 29 30 31Strain fitness (log2 ratio) -2 -1 0 1at 28.433 kb on - strand, within GFF2901at 28.468 kb on - strand, within GFF2901at 28.519 kb on + strand, within GFF2901at 28.850 kb on - strand, within GFF2901at 28.930 kb on - strand, within GFF2901at 28.936 kb on + strand, within GFF2901at 29.162 kb on - strandat 29.164 kb on + strandat 29.193 kb on + strandat 29.197 kb on + strandat 29.197 kb on + strandat 29.198 kb on - strandat 29.198 kb on - strandat 29.234 kb on + strandat 29.234 kb on + strandat 29.235 kb on - strandat 29.281 kb on + strandat 29.289 kb on + strandat 29.289 kb on + strandat 29.290 kb on - strandat 29.532 kb on - strand, within GFF2902at 29.591 kb on + strand, within GFF2902at 29.730 kb on + strand, within GFF2902at 29.800 kb on + strand, within GFF2902at 29.905 kb on + strand, within GFF2902at 29.906 kb on - strand, within GFF2902at 29.948 kb on - strand, within GFF2902at 29.991 kb on + strand, within GFF2902at 30.007 kb on + strand, within GFF2902at 30.007 kb on + strand, within GFF2902at 30.007 kb on + strand, within GFF2902at 30.122 kb on + strand, within GFF2902at 30.122 kb on + strand, within GFF2902at 30.122 kb on + strand, within GFF2902at 30.122 kb on + strand, within GFF2902at 30.123 kb on - strand, within GFF2902at 30.123 kb on - strand, within GFF2902at 30.195 kb on - strand, within GFF2902at 30.271 kb on + strandat 30.380 kb on - strand, within GFF2903at 30.383 kb on + strand, within GFF2903at 30.384 kb on - strand, within GFF2903at 30.384 kb on - strand, within GFF2903at 30.391 kb on + strand, within GFF2903at 30.392 kb on - strand, within GFF2903at 30.399 kb on - strand, within GFF2903at 30.440 kb on + strand, within GFF2903at 30.524 kb on + strand, within GFF2903at 30.525 kb on - strand, within GFF2903at 30.552 kb on - strandat 30.718 kb on - strandat 30.724 kb on - strandat 30.862 kb on + strand, within GFF2904at 30.896 kb on - strand, within GFF2904at 30.945 kb on + strand, within GFF2904at 31.055 kb on + strand, within GFF2904at 31.056 kb on - strand, within GFF2904at 31.056 kb on - strand, within GFF2904at 31.062 kb on + strand, within GFF2904at 31.062 kb on + strand, within GFF2904at 31.062 kb on + strand, within GFF2904at 31.062 kb on + strand, within GFF2904at 31.063 kb on - strand, within GFF2904at 31.063 kb on - strand, within GFF2904at 31.160 kb on + strand, within GFF2904

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.00005 MOI
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28,433 - GFF2901 0.58 +0.6
28,468 - GFF2901 0.60 +0.4
28,519 + GFF2901 0.63 +0.2
28,850 - GFF2901 0.81 +0.0
28,930 - GFF2901 0.85 +1.7
28,936 + GFF2901 0.86 +1.4
29,162 - -0.2
29,164 + +0.4
29,193 + -0.1
29,197 + +0.4
29,197 + +0.5
29,198 - +0.5
29,198 - +0.4
29,234 + +0.6
29,234 + +0.4
29,235 - +0.8
29,281 + -1.3
29,289 + -0.3
29,289 + +0.5
29,290 - +0.4
29,532 - GFF2902 0.18 +0.1
29,591 + GFF2902 0.24 +0.1
29,730 + GFF2902 0.39 +0.0
29,800 + GFF2902 0.47 +0.1
29,905 + GFF2902 0.58 -0.6
29,906 - GFF2902 0.58 +1.1
29,948 - GFF2902 0.63 +0.4
29,991 + GFF2902 0.67 -0.0
30,007 + GFF2902 0.69 +0.3
30,007 + GFF2902 0.69 +0.5
30,007 + GFF2902 0.69 +0.4
30,122 + GFF2902 0.82 +1.3
30,122 + GFF2902 0.82 +0.8
30,122 + GFF2902 0.82 +0.7
30,122 + GFF2902 0.82 +0.3
30,123 - GFF2902 0.82 +1.0
30,123 - GFF2902 0.82 +0.3
30,195 - GFF2902 0.89 -1.3
30,271 + +0.4
30,380 - GFF2903 0.26 +0.6
30,383 + GFF2903 0.27 +0.2
30,384 - GFF2903 0.28 +0.4
30,384 - GFF2903 0.28 +0.1
30,391 + GFF2903 0.30 +0.6
30,392 - GFF2903 0.31 +0.3
30,399 - GFF2903 0.33 +0.3
30,440 + GFF2903 0.49 +0.2
30,524 + GFF2903 0.81 +0.2
30,525 - GFF2903 0.81 +0.7
30,552 - -1.2
30,718 - +0.3
30,724 - -0.9
30,862 + GFF2904 0.18 -0.0
30,896 - GFF2904 0.20 -0.5
30,945 + GFF2904 0.23 +0.3
31,055 + GFF2904 0.29 -0.4
31,056 - GFF2904 0.29 +0.7
31,056 - GFF2904 0.29 +0.4
31,062 + GFF2904 0.30 -2.2
31,062 + GFF2904 0.30 +0.2
31,062 + GFF2904 0.30 +0.0
31,062 + GFF2904 0.30 +0.3
31,063 - GFF2904 0.30 +0.3
31,063 - GFF2904 0.30 -0.6
31,160 + GFF2904 0.36 +0.4

Or see this region's nucleotide sequence