Experiment: LB_plus_SM_buffer with Br60_phage 0.00005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1897 and GFF1898 are separated by 10 nucleotides GFF1898 and GFF1899 are separated by 37 nucleotides GFF1899 and GFF1900 are separated by 339 nucleotides
GFF1897 - Putative phosphatase YfbT, at 92,287 to 92,946
GFF1897
GFF1898 - FIG00361523: hypothetical protein, at 92,957 to 93,496
GFF1898
GFF1899 - FIG00638298: membrane protein YfbV, at 93,534 to 93,989
GFF1899
GFF1900 - Acetate kinase (EC 2.7.2.1), at 94,329 to 95,531
GFF1900
Position (kb) 93
94 Strain fitness (log2 ratio) -1
0
1
2 at 92.659 kb on - strand, within GFF1897 at 92.659 kb on - strand, within GFF1897 at 92.725 kb on + strand, within GFF1897 at 92.726 kb on - strand, within GFF1897 at 92.868 kb on + strand, within GFF1897 at 93.113 kb on + strand, within GFF1898 at 93.113 kb on + strand, within GFF1898 at 93.114 kb on - strand, within GFF1898 at 93.115 kb on + strand, within GFF1898 at 93.135 kb on + strand, within GFF1898 at 93.135 kb on + strand, within GFF1898 at 93.246 kb on + strand, within GFF1898 at 93.247 kb on - strand, within GFF1898 at 93.247 kb on - strand, within GFF1898 at 93.247 kb on - strand, within GFF1898 at 93.247 kb on - strand, within GFF1898 at 93.272 kb on - strand, within GFF1898 at 93.409 kb on - strand, within GFF1898 at 93.512 kb on + strand at 93.557 kb on + strand at 93.582 kb on - strand, within GFF1899 at 93.594 kb on + strand, within GFF1899 at 93.671 kb on - strand, within GFF1899 at 93.801 kb on + strand, within GFF1899 at 93.854 kb on - strand, within GFF1899 at 93.949 kb on - strand at 94.086 kb on - strand at 94.154 kb on + strand at 94.154 kb on + strand at 94.154 kb on + strand at 94.154 kb on + strand at 94.154 kb on + strand at 94.155 kb on - strand at 94.155 kb on - strand at 94.202 kb on + strand at 94.203 kb on - strand at 94.250 kb on + strand at 94.310 kb on - strand at 94.318 kb on + strand at 94.318 kb on + strand at 94.319 kb on - strand at 94.323 kb on - strand at 94.338 kb on - strand at 94.548 kb on - strand, within GFF1900 at 94.583 kb on - strand, within GFF1900 at 94.583 kb on - strand, within GFF1900 at 94.583 kb on - strand, within GFF1900 at 94.607 kb on - strand, within GFF1900 at 94.625 kb on + strand, within GFF1900 at 94.812 kb on + strand, within GFF1900 at 94.813 kb on - strand, within GFF1900 at 94.841 kb on + strand, within GFF1900 at 94.842 kb on - strand, within GFF1900 at 94.890 kb on + strand, within GFF1900 at 94.891 kb on - strand, within GFF1900 at 94.946 kb on + strand, within GFF1900
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.00005 MOI remove 92,659 - GFF1897 0.56 -0.9 92,659 - GFF1897 0.56 +0.5 92,725 + GFF1897 0.66 +0.3 92,726 - GFF1897 0.67 +0.0 92,868 + GFF1897 0.88 +0.4 93,113 + GFF1898 0.29 -0.5 93,113 + GFF1898 0.29 -0.1 93,114 - GFF1898 0.29 -0.3 93,115 + GFF1898 0.29 +0.1 93,135 + GFF1898 0.33 +0.2 93,135 + GFF1898 0.33 -1.2 93,246 + GFF1898 0.54 -0.8 93,247 - GFF1898 0.54 +0.4 93,247 - GFF1898 0.54 +0.6 93,247 - GFF1898 0.54 -0.0 93,247 - GFF1898 0.54 +0.3 93,272 - GFF1898 0.58 +0.7 93,409 - GFF1898 0.84 +1.1 93,512 + +0.4 93,557 + +0.7 93,582 - GFF1899 0.11 +0.4 93,594 + GFF1899 0.13 +0.3 93,671 - GFF1899 0.30 +0.4 93,801 + GFF1899 0.59 +1.3 93,854 - GFF1899 0.70 +0.3 93,949 - +0.8 94,086 - +0.2 94,154 + -0.1 94,154 + +0.1 94,154 + +0.4 94,154 + +0.2 94,154 + +0.1 94,155 - -0.4 94,155 - +0.8 94,202 + +0.1 94,203 - +0.0 94,250 + +0.0 94,310 - +0.5 94,318 + +0.9 94,318 + +0.2 94,319 - -0.3 94,323 - -0.5 94,338 - -0.6 94,548 - GFF1900 0.18 -0.4 94,583 - GFF1900 0.21 +0.3 94,583 - GFF1900 0.21 +0.0 94,583 - GFF1900 0.21 -0.4 94,607 - GFF1900 0.23 -0.1 94,625 + GFF1900 0.25 +0.5 94,812 + GFF1900 0.40 +0.1 94,813 - GFF1900 0.40 -1.6 94,841 + GFF1900 0.43 +0.2 94,842 - GFF1900 0.43 +0.2 94,890 + GFF1900 0.47 +0.1 94,891 - GFF1900 0.47 +0.5 94,946 + GFF1900 0.51 +2.3
Or see this region's nucleotide sequence