Experiment: LB_plus_SM_buffer with Br60_phage 0.00005 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1807 and GFF1808 are separated by 199 nucleotides GFF1808 and GFF1809 are separated by 102 nucleotides
GFF1807 - Fumarate hydratase class I, aerobic (EC 4.2.1.2), at 29,952 to 31,598
GFF1807
GFF1808 - Mannose-6-phosphate isomerase (EC 5.3.1.8), at 31,798 to 32,973
GFF1808
GFF1809 - Putative GTP-binding protein YdgA, at 33,076 to 34,584
GFF1809
Position (kb)
31
32
33 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 30.842 kb on - strand, within GFF1807 at 30.842 kb on - strand, within GFF1807 at 30.844 kb on + strand, within GFF1807 at 30.845 kb on - strand, within GFF1807 at 30.947 kb on + strand, within GFF1807 at 31.012 kb on + strand, within GFF1807 at 31.019 kb on - strand, within GFF1807 at 31.065 kb on - strand, within GFF1807 at 31.113 kb on - strand, within GFF1807 at 31.135 kb on + strand, within GFF1807 at 31.135 kb on + strand, within GFF1807 at 31.136 kb on - strand, within GFF1807 at 31.183 kb on + strand, within GFF1807 at 31.184 kb on - strand, within GFF1807 at 31.184 kb on - strand, within GFF1807 at 31.184 kb on - strand, within GFF1807 at 31.184 kb on - strand, within GFF1807 at 31.190 kb on + strand, within GFF1807 at 31.192 kb on + strand, within GFF1807 at 31.193 kb on - strand, within GFF1807 at 31.193 kb on - strand, within GFF1807 at 31.262 kb on + strand, within GFF1807 at 31.263 kb on - strand, within GFF1807 at 31.345 kb on + strand, within GFF1807 at 31.346 kb on - strand, within GFF1807 at 31.414 kb on - strand, within GFF1807 at 31.419 kb on - strand, within GFF1807 at 31.419 kb on - strand, within GFF1807 at 31.419 kb on - strand, within GFF1807 at 31.534 kb on + strand at 31.535 kb on - strand at 31.674 kb on + strand at 31.759 kb on + strand at 32.174 kb on - strand, within GFF1808 at 32.174 kb on - strand, within GFF1808 at 32.566 kb on + strand, within GFF1808 at 32.683 kb on - strand, within GFF1808 at 32.683 kb on - strand, within GFF1808 at 32.856 kb on + strand at 32.856 kb on + strand at 32.980 kb on - strand at 33.011 kb on - strand at 33.092 kb on + strand at 33.108 kb on - strand at 33.319 kb on - strand, within GFF1809 at 33.418 kb on - strand, within GFF1809 at 33.501 kb on + strand, within GFF1809 at 33.520 kb on + strand, within GFF1809 at 33.718 kb on - strand, within GFF1809
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Br60_phage 0.00005 MOI remove 30,842 - GFF1807 0.54 +1.0 30,842 - GFF1807 0.54 -0.0 30,844 + GFF1807 0.54 -1.0 30,845 - GFF1807 0.54 -0.2 30,947 + GFF1807 0.60 +0.2 31,012 + GFF1807 0.64 -0.4 31,019 - GFF1807 0.65 +0.1 31,065 - GFF1807 0.68 -0.9 31,113 - GFF1807 0.70 -0.2 31,135 + GFF1807 0.72 -0.8 31,135 + GFF1807 0.72 -2.5 31,136 - GFF1807 0.72 +0.5 31,183 + GFF1807 0.75 -0.1 31,184 - GFF1807 0.75 +0.3 31,184 - GFF1807 0.75 +0.4 31,184 - GFF1807 0.75 -1.0 31,184 - GFF1807 0.75 -0.0 31,190 + GFF1807 0.75 +2.2 31,192 + GFF1807 0.75 -0.0 31,193 - GFF1807 0.75 +0.6 31,193 - GFF1807 0.75 +0.0 31,262 + GFF1807 0.80 -0.2 31,263 - GFF1807 0.80 -0.8 31,345 + GFF1807 0.85 +0.0 31,346 - GFF1807 0.85 -0.0 31,414 - GFF1807 0.89 +0.3 31,419 - GFF1807 0.89 +0.1 31,419 - GFF1807 0.89 -0.6 31,419 - GFF1807 0.89 +0.2 31,534 + -0.0 31,535 - +0.0 31,674 + -0.2 31,759 + -0.5 32,174 - GFF1808 0.32 -1.4 32,174 - GFF1808 0.32 -2.3 32,566 + GFF1808 0.65 -1.5 32,683 - GFF1808 0.75 -0.2 32,683 - GFF1808 0.75 -1.8 32,856 + -2.3 32,856 + -0.9 32,980 - -0.3 33,011 - -0.6 33,092 + -0.0 33,108 - +0.1 33,319 - GFF1809 0.16 -0.3 33,418 - GFF1809 0.23 +0.2 33,501 + GFF1809 0.28 +0.1 33,520 + GFF1809 0.29 +0.1 33,718 - GFF1809 0.43 +0.4
Or see this region's nucleotide sequence