Experiment: R2A with Ceftazidime 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu3732 and Atu3733 are separated by 2 nucleotides Atu3733 and Atu8191 are separated by 236 nucleotides Atu8191 and Atu8192 are separated by 11 nucleotides Atu8192 and Atu3736 are separated by 323 nucleotides
Atu3732: Atu3732 - hemolysin, at 812,359 to 813,636
Atu3732
Atu3733: Atu3733 - hypothetical protein, at 813,639 to 813,851
Atu3733
Atu8191: Atu8191 - hypothetical protein, at 814,088 to 814,465
Atu8191
Atu8192: Atu8192 - hypothetical protein, at 814,477 to 814,749
Atu8192
Atu3736: Atu3736 - transketolase, at 815,073 to 817,055
Atu3736
Position (kb) 814
815 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 813.116 kb on + strand, within Atu3732 at 813.116 kb on + strand, within Atu3732 at 813.124 kb on - strand, within Atu3732 at 813.124 kb on - strand, within Atu3732 at 813.124 kb on - strand, within Atu3732 at 813.124 kb on - strand, within Atu3732 at 813.124 kb on - strand, within Atu3732 at 813.222 kb on + strand, within Atu3732 at 813.367 kb on + strand, within Atu3732 at 813.436 kb on - strand, within Atu3732 at 813.512 kb on + strand at 813.542 kb on - strand at 813.590 kb on + strand at 813.598 kb on - strand at 813.607 kb on - strand at 813.654 kb on + strand at 813.662 kb on - strand, within Atu3733 at 813.780 kb on - strand, within Atu3733 at 813.840 kb on - strand at 813.920 kb on + strand at 813.972 kb on - strand at 814.026 kb on + strand at 814.037 kb on + strand at 814.134 kb on - strand, within Atu8191 at 814.425 kb on + strand, within Atu8191 at 814.483 kb on + strand at 814.586 kb on - strand, within Atu8192 at 814.824 kb on - strand at 814.826 kb on + strand at 814.847 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Ceftazidime 4 mM remove 813,116 + Atu3732 0.59 +0.9 813,116 + Atu3732 0.59 -1.1 813,124 - Atu3732 0.60 +2.5 813,124 - Atu3732 0.60 +0.8 813,124 - Atu3732 0.60 -0.5 813,124 - Atu3732 0.60 +0.1 813,124 - Atu3732 0.60 +0.5 813,222 + Atu3732 0.68 +1.1 813,367 + Atu3732 0.79 +0.2 813,436 - Atu3732 0.84 +0.7 813,512 + +0.5 813,542 - +0.6 813,590 + +0.9 813,598 - +0.5 813,607 - +0.3 813,654 + +0.4 813,662 - Atu3733 0.11 +0.5 813,780 - Atu3733 0.66 -1.4 813,840 - -0.1 813,920 + +0.4 813,972 - +1.2 814,026 + +0.8 814,037 + +1.1 814,134 - Atu8191 0.12 -0.7 814,425 + Atu8191 0.89 -1.1 814,483 + -1.6 814,586 - Atu8192 0.40 -3.1 814,824 - -0.7 814,826 + -1.4 814,847 + +0.2
Or see this region's nucleotide sequence