Experiment: R2A with Ceftazidime 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu4650 and Atu4651 overlap by 1 nucleotides Atu4651 and Atu4652 are separated by 148 nucleotides Atu4652 and Atu4653 overlap by 4 nucleotides
Atu4650: Atu4650 - sugar ABC transporter ATPase, at 1,803,714 to 1,804,754
Atu4650
Atu4651: Atu4651 - argininosuccinate lyase, at 1,804,754 to 1,806,124
Atu4651
Atu4652: Atu4652 - sugar ABC transporter permease, at 1,806,273 to 1,807,115
Atu4652
Atu4653: Atu4653 - sugar ABC transporter permease, at 1,807,112 to 1,808,053
Atu4653
Position (kb)
1804
1805
1806
1807 Strain fitness (log2 ratio)
-2
-1
0
1
2
3 at 1803.856 kb on + strand, within Atu4650 at 1803.919 kb on + strand, within Atu4650 at 1803.972 kb on + strand, within Atu4650 at 1804.095 kb on - strand, within Atu4650 at 1804.251 kb on + strand, within Atu4650 at 1804.310 kb on + strand, within Atu4650 at 1804.358 kb on - strand, within Atu4650 at 1804.382 kb on + strand, within Atu4650 at 1804.543 kb on - strand, within Atu4650 at 1804.685 kb on + strand at 1804.685 kb on + strand at 1804.686 kb on + strand at 1804.692 kb on + strand at 1804.693 kb on - strand at 1804.693 kb on - strand at 1804.694 kb on - strand at 1804.694 kb on - strand at 1804.764 kb on + strand at 1804.792 kb on + strand at 1804.936 kb on + strand, within Atu4651 at 1805.106 kb on - strand, within Atu4651 at 1805.118 kb on - strand, within Atu4651 at 1805.185 kb on + strand, within Atu4651 at 1805.193 kb on - strand, within Atu4651 at 1805.193 kb on - strand, within Atu4651 at 1805.196 kb on + strand, within Atu4651 at 1805.196 kb on + strand, within Atu4651 at 1805.204 kb on - strand, within Atu4651 at 1805.204 kb on - strand, within Atu4651 at 1805.263 kb on - strand, within Atu4651 at 1805.334 kb on - strand, within Atu4651 at 1805.345 kb on - strand, within Atu4651 at 1805.397 kb on - strand, within Atu4651 at 1805.629 kb on - strand, within Atu4651 at 1805.647 kb on - strand, within Atu4651 at 1805.648 kb on + strand, within Atu4651 at 1805.648 kb on + strand, within Atu4651 at 1805.765 kb on + strand, within Atu4651 at 1805.768 kb on - strand, within Atu4651 at 1805.846 kb on - strand, within Atu4651 at 1805.877 kb on + strand, within Atu4651 at 1805.903 kb on - strand, within Atu4651 at 1805.937 kb on + strand, within Atu4651 at 1805.937 kb on + strand, within Atu4651 at 1805.945 kb on - strand, within Atu4651 at 1805.999 kb on + strand at 1806.028 kb on - strand at 1806.042 kb on + strand at 1806.125 kb on + strand at 1806.130 kb on + strand at 1806.133 kb on - strand at 1806.155 kb on + strand at 1806.265 kb on - strand at 1806.293 kb on + strand at 1806.341 kb on + strand at 1806.349 kb on - strand at 1806.411 kb on + strand, within Atu4652 at 1806.432 kb on + strand, within Atu4652 at 1806.433 kb on + strand, within Atu4652 at 1806.433 kb on + strand, within Atu4652 at 1806.441 kb on - strand, within Atu4652 at 1806.441 kb on - strand, within Atu4652 at 1806.471 kb on + strand, within Atu4652 at 1806.479 kb on - strand, within Atu4652 at 1806.479 kb on - strand, within Atu4652 at 1806.479 kb on - strand, within Atu4652 at 1806.506 kb on - strand, within Atu4652 at 1806.556 kb on - strand, within Atu4652 at 1806.592 kb on + strand, within Atu4652 at 1806.726 kb on - strand, within Atu4652 at 1806.727 kb on + strand, within Atu4652 at 1806.815 kb on + strand, within Atu4652 at 1806.815 kb on + strand, within Atu4652 at 1806.815 kb on + strand, within Atu4652 at 1806.823 kb on - strand, within Atu4652 at 1806.823 kb on - strand, within Atu4652 at 1806.858 kb on + strand, within Atu4652 at 1806.875 kb on + strand, within Atu4652 at 1806.902 kb on - strand, within Atu4652 at 1806.907 kb on - strand, within Atu4652 at 1806.958 kb on + strand, within Atu4652 at 1806.975 kb on - strand, within Atu4652 at 1807.034 kb on + strand at 1807.044 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Ceftazidime 4 mM remove 1,803,856 + Atu4650 0.14 +0.2 1,803,919 + Atu4650 0.20 +0.1 1,803,972 + Atu4650 0.25 -0.0 1,804,095 - Atu4650 0.37 -0.7 1,804,251 + Atu4650 0.52 +0.5 1,804,310 + Atu4650 0.57 -0.2 1,804,358 - Atu4650 0.62 -0.3 1,804,382 + Atu4650 0.64 -0.1 1,804,543 - Atu4650 0.80 -0.1 1,804,685 + -0.5 1,804,685 + +0.1 1,804,686 + +0.2 1,804,692 + +0.2 1,804,693 - +3.0 1,804,693 - +0.3 1,804,694 - +0.1 1,804,694 - -0.0 1,804,764 + -0.1 1,804,792 + -0.1 1,804,936 + Atu4651 0.13 -0.1 1,805,106 - Atu4651 0.26 -0.8 1,805,118 - Atu4651 0.27 +0.6 1,805,185 + Atu4651 0.31 -0.5 1,805,193 - Atu4651 0.32 -0.3 1,805,193 - Atu4651 0.32 +0.4 1,805,196 + Atu4651 0.32 -0.1 1,805,196 + Atu4651 0.32 -0.1 1,805,204 - Atu4651 0.33 -0.6 1,805,204 - Atu4651 0.33 -0.6 1,805,263 - Atu4651 0.37 -0.2 1,805,334 - Atu4651 0.42 -1.8 1,805,345 - Atu4651 0.43 +0.1 1,805,397 - Atu4651 0.47 +0.1 1,805,629 - Atu4651 0.64 -0.1 1,805,647 - Atu4651 0.65 +0.3 1,805,648 + Atu4651 0.65 -0.0 1,805,648 + Atu4651 0.65 -0.6 1,805,765 + Atu4651 0.74 -0.4 1,805,768 - Atu4651 0.74 +0.4 1,805,846 - Atu4651 0.80 -0.3 1,805,877 + Atu4651 0.82 +0.2 1,805,903 - Atu4651 0.84 -1.0 1,805,937 + Atu4651 0.86 -1.2 1,805,937 + Atu4651 0.86 -0.8 1,805,945 - Atu4651 0.87 -1.5 1,805,999 + -0.1 1,806,028 - +0.8 1,806,042 + -0.3 1,806,125 + +0.5 1,806,130 + -0.3 1,806,133 - -0.2 1,806,155 + +0.4 1,806,265 - -0.1 1,806,293 + +0.3 1,806,341 + +0.6 1,806,349 - -1.1 1,806,411 + Atu4652 0.16 -0.0 1,806,432 + Atu4652 0.19 +0.0 1,806,433 + Atu4652 0.19 +1.1 1,806,433 + Atu4652 0.19 -0.1 1,806,441 - Atu4652 0.20 -0.0 1,806,441 - Atu4652 0.20 -0.2 1,806,471 + Atu4652 0.23 +1.0 1,806,479 - Atu4652 0.24 +0.4 1,806,479 - Atu4652 0.24 -0.5 1,806,479 - Atu4652 0.24 +0.6 1,806,506 - Atu4652 0.28 +0.0 1,806,556 - Atu4652 0.34 -0.1 1,806,592 + Atu4652 0.38 -0.2 1,806,726 - Atu4652 0.54 -0.9 1,806,727 + Atu4652 0.54 -0.7 1,806,815 + Atu4652 0.64 +0.1 1,806,815 + Atu4652 0.64 -0.0 1,806,815 + Atu4652 0.64 -0.3 1,806,823 - Atu4652 0.65 +0.2 1,806,823 - Atu4652 0.65 -0.5 1,806,858 + Atu4652 0.69 -0.1 1,806,875 + Atu4652 0.71 -0.8 1,806,902 - Atu4652 0.75 -1.0 1,806,907 - Atu4652 0.75 -0.8 1,806,958 + Atu4652 0.81 -1.9 1,806,975 - Atu4652 0.83 +1.5 1,807,034 + +0.3 1,807,044 + +0.6
Or see this region's nucleotide sequence