Experiment: R2A with Ceftazidime 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu1174 and Atu1175 are separated by 74 nucleotides Atu1175 and Atu1176 are separated by 272 nucleotides Atu1176 and Atu1177 are separated by 11 nucleotides
Atu1174: Atu1174 - H+ translocating pyrophosphate synthase, at 1,167,264 to 1,169,402
Atu1174
Atu1175: Atu1175 - hypothetical protein, at 1,169,477 to 1,170,010
Atu1175
Atu1176: Atu1176 - hypothetical protein, at 1,170,283 to 1,170,819
Atu1176
Atu1177: Atu1177 - hypothetical protein, at 1,170,831 to 1,171,394
Atu1177
Position (kb)
1169
1170
1171 Strain fitness (log2 ratio)
-1
0
1
2 at 1168.528 kb on - strand, within Atu1174 at 1168.533 kb on - strand, within Atu1174 at 1168.670 kb on + strand, within Atu1174 at 1168.676 kb on + strand, within Atu1174 at 1168.708 kb on + strand, within Atu1174 at 1168.811 kb on + strand, within Atu1174 at 1168.849 kb on + strand, within Atu1174 at 1168.855 kb on + strand, within Atu1174 at 1168.865 kb on - strand, within Atu1174 at 1168.953 kb on + strand, within Atu1174 at 1169.028 kb on - strand, within Atu1174 at 1169.131 kb on - strand, within Atu1174 at 1169.206 kb on - strand at 1169.245 kb on + strand at 1169.252 kb on - strand at 1169.411 kb on - strand at 1169.412 kb on - strand at 1169.546 kb on - strand, within Atu1175 at 1170.118 kb on - strand at 1170.406 kb on + strand, within Atu1176 at 1170.559 kb on + strand, within Atu1176 at 1170.645 kb on + strand, within Atu1176 at 1170.663 kb on - strand, within Atu1176 at 1170.680 kb on + strand, within Atu1176 at 1170.680 kb on + strand, within Atu1176 at 1170.767 kb on + strand at 1170.768 kb on + strand at 1170.776 kb on - strand at 1170.812 kb on - strand at 1170.824 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Ceftazidime 4 mM remove 1,168,528 - Atu1174 0.59 +0.4 1,168,533 - Atu1174 0.59 +0.8 1,168,670 + Atu1174 0.66 -0.4 1,168,676 + Atu1174 0.66 +0.1 1,168,708 + Atu1174 0.68 -0.1 1,168,811 + Atu1174 0.72 -0.2 1,168,849 + Atu1174 0.74 -0.8 1,168,855 + Atu1174 0.74 +0.2 1,168,865 - Atu1174 0.75 -0.3 1,168,953 + Atu1174 0.79 +0.2 1,169,028 - Atu1174 0.82 +0.9 1,169,131 - Atu1174 0.87 +1.9 1,169,206 - -0.0 1,169,245 + -0.9 1,169,252 - +1.4 1,169,411 - -0.7 1,169,412 - +2.5 1,169,546 - Atu1175 0.13 -1.2 1,170,118 - -0.7 1,170,406 + Atu1176 0.23 +0.6 1,170,559 + Atu1176 0.51 +0.3 1,170,645 + Atu1176 0.67 -0.5 1,170,663 - Atu1176 0.71 -0.7 1,170,680 + Atu1176 0.74 +0.1 1,170,680 + Atu1176 0.74 +1.2 1,170,767 + -1.1 1,170,768 + -0.3 1,170,776 - +0.6 1,170,812 - +1.2 1,170,824 - +1.9
Or see this region's nucleotide sequence