Experiment: R2A with Ceftazidime 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0255 and Atu0256 are separated by 40 nucleotides Atu0256 and Atu0257 are separated by 164 nucleotides Atu0257 and Atu0258 are separated by 25 nucleotides
Atu0255: Atu0255 - 50S ribosomal protein L35, at 252,753 to 252,956
Atu0255
Atu0256: Atu0256 - 50S ribosomal protein L20, at 252,997 to 253,401
Atu0256
Atu0257: Atu0257 - hypothetical protein, at 253,566 to 254,444
Atu0257
Atu0258: Atu0258 - phenylalanyl-tRNA synthetase, alpha-subunit, at 254,470 to 255,519
Atu0258
Position (kb) 253
254
255 Strain fitness (log2 ratio) -2
-1
0
1 at 252.593 kb on - strand at 253.537 kb on + strand at 253.639 kb on - strand at 253.661 kb on - strand, within Atu0257 at 253.716 kb on - strand, within Atu0257 at 253.786 kb on - strand, within Atu0257 at 253.798 kb on + strand, within Atu0257 at 253.816 kb on + strand, within Atu0257 at 253.824 kb on - strand, within Atu0257 at 253.995 kb on + strand, within Atu0257 at 254.024 kb on + strand, within Atu0257 at 254.032 kb on + strand, within Atu0257 at 254.040 kb on - strand, within Atu0257 at 254.143 kb on - strand, within Atu0257 at 254.158 kb on - strand, within Atu0257 at 254.195 kb on - strand, within Atu0257 at 254.225 kb on - strand, within Atu0257 at 254.236 kb on + strand, within Atu0257 at 254.252 kb on + strand, within Atu0257 at 254.262 kb on + strand, within Atu0257 at 254.268 kb on + strand, within Atu0257 at 254.336 kb on + strand, within Atu0257
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Ceftazidime 4 mM remove 252,593 - +1.2 253,537 + +0.6 253,639 - -0.6 253,661 - Atu0257 0.11 -1.0 253,716 - Atu0257 0.17 -1.9 253,786 - Atu0257 0.25 -0.6 253,798 + Atu0257 0.26 -1.2 253,816 + Atu0257 0.28 -1.1 253,824 - Atu0257 0.29 -0.9 253,995 + Atu0257 0.49 -1.2 254,024 + Atu0257 0.52 -1.1 254,032 + Atu0257 0.53 +0.3 254,040 - Atu0257 0.54 -1.2 254,143 - Atu0257 0.66 -0.2 254,158 - Atu0257 0.67 -1.6 254,195 - Atu0257 0.72 +1.2 254,225 - Atu0257 0.75 -0.7 254,236 + Atu0257 0.76 -1.0 254,252 + Atu0257 0.78 -1.9 254,262 + Atu0257 0.79 -1.9 254,268 + Atu0257 0.80 -0.8 254,336 + Atu0257 0.88 +0.3
Or see this region's nucleotide sequence