Experiment: R2A with Ceftazidime 4 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt Atu0253 and Atu0254 are separated by 301 nucleotides Atu0254 and Atu0255 are separated by 189 nucleotides Atu0255 and Atu0256 are separated by 40 nucleotides
Atu0253: Atu0253 - hypothetical protein, at 251,051 to 251,839
Atu0253
Atu0254: Atu0254 - translation initiation factor 3, at 252,141 to 252,563
Atu0254
Atu0255: Atu0255 - 50S ribosomal protein L35, at 252,753 to 252,956
Atu0255
Atu0256: Atu0256 - 50S ribosomal protein L20, at 252,997 to 253,401
Atu0256
Position (kb) 252
253 Strain fitness (log2 ratio) -2
-1
0
1 at 251.162 kb on - strand, within Atu0253 at 251.252 kb on - strand, within Atu0253 at 251.267 kb on + strand, within Atu0253 at 251.275 kb on - strand, within Atu0253 at 251.297 kb on + strand, within Atu0253 at 251.298 kb on - strand, within Atu0253 at 251.316 kb on + strand, within Atu0253 at 251.332 kb on - strand, within Atu0253 at 251.353 kb on + strand, within Atu0253 at 251.421 kb on + strand, within Atu0253 at 251.471 kb on - strand, within Atu0253 at 251.554 kb on + strand, within Atu0253 at 251.563 kb on + strand, within Atu0253 at 251.724 kb on + strand, within Atu0253 at 251.732 kb on - strand, within Atu0253 at 251.732 kb on - strand, within Atu0253 at 251.747 kb on + strand, within Atu0253 at 251.801 kb on - strand at 251.839 kb on + strand at 251.847 kb on - strand at 252.593 kb on - strand at 253.537 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A with Ceftazidime 4 mM remove 251,162 - Atu0253 0.14 +0.5 251,252 - Atu0253 0.25 -0.4 251,267 + Atu0253 0.27 -0.2 251,275 - Atu0253 0.28 +0.6 251,297 + Atu0253 0.31 -2.4 251,298 - Atu0253 0.31 -0.1 251,316 + Atu0253 0.34 +0.0 251,332 - Atu0253 0.36 +1.3 251,353 + Atu0253 0.38 +0.1 251,421 + Atu0253 0.47 -0.4 251,471 - Atu0253 0.53 -0.1 251,554 + Atu0253 0.64 +0.5 251,563 + Atu0253 0.65 -0.7 251,724 + Atu0253 0.85 +0.5 251,732 - Atu0253 0.86 -1.0 251,732 - Atu0253 0.86 -0.3 251,747 + Atu0253 0.88 +0.2 251,801 - -0.5 251,839 + -0.8 251,847 - -0.4 252,593 - +1.2 253,537 + +0.6
Or see this region's nucleotide sequence