Experiment: capsaicin 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt yeaP and yoaK are separated by 32 nucleotides yoaK and yoaJ are separated by 2 nucleotides yoaJ and yeaQ are separated by 58 nucleotides yeaQ and yoaG are separated by 147 nucleotides yoaG and yeaR are separated by 3 nucleotides yeaR and yeaS are separated by 172 nucleotides
b1794: yeaP - orf, hypothetical protein (VIMSS), at 1,875,739 to 1,876,764
yeaP
b4676: yoaK - expressed protein (RefSeq), at 1,876,797 to 1,876,895
yoaK
b4675: yoaJ - expressed protein (RefSeq), at 1,876,898 to 1,876,972
yoaJ
b1795: yeaQ - conserved inner membrane protein (NCBI), at 1,877,031 to 1,877,279
yeaQ
b1796: yoaG - hypothetical protein (NCBI), at 1,877,427 to 1,877,609
yoaG
b1797: yeaR - hypothetical protein (NCBI), at 1,877,613 to 1,877,972
yeaR
b1798: yeaS - neutral amino-acid efflux system (NCBI), at 1,878,145 to 1,878,783
yeaS
Position (kb)
1877
1878 Strain fitness (log2 ratio)
-1
0
1 at 1876.452 kb on + strand, within yeaP at 1876.480 kb on + strand, within yeaP at 1876.506 kb on + strand, within yeaP at 1876.616 kb on + strand, within yeaP at 1876.639 kb on + strand, within yeaP at 1876.639 kb on + strand, within yeaP at 1876.668 kb on + strand at 1876.678 kb on - strand at 1876.707 kb on + strand at 1876.753 kb on + strand at 1876.753 kb on + strand at 1876.753 kb on + strand at 1876.770 kb on + strand at 1876.879 kb on - strand, within yoaK at 1876.879 kb on - strand, within yoaK at 1876.889 kb on - strand at 1876.889 kb on - strand at 1876.945 kb on + strand, within yoaJ at 1876.945 kb on + strand, within yoaJ at 1876.955 kb on + strand, within yoaJ at 1876.963 kb on - strand, within yoaJ at 1876.963 kb on - strand, within yoaJ at 1877.005 kb on + strand at 1877.014 kb on - strand at 1877.035 kb on + strand at 1877.135 kb on - strand, within yeaQ at 1877.137 kb on + strand, within yeaQ at 1877.145 kb on - strand, within yeaQ at 1877.145 kb on - strand, within yeaQ at 1877.174 kb on - strand, within yeaQ at 1877.175 kb on - strand, within yeaQ at 1877.260 kb on + strand at 1877.433 kb on + strand at 1877.476 kb on + strand, within yoaG at 1877.476 kb on + strand, within yoaG at 1877.484 kb on - strand, within yoaG at 1877.513 kb on - strand, within yoaG at 1877.571 kb on - strand, within yoaG at 1877.602 kb on - strand at 1877.658 kb on - strand, within yeaR at 1877.663 kb on + strand, within yeaR at 1877.663 kb on + strand, within yeaR at 1877.714 kb on + strand, within yeaR at 1877.715 kb on - strand, within yeaR at 1877.764 kb on + strand, within yeaR at 1877.765 kb on + strand, within yeaR at 1877.765 kb on + strand, within yeaR at 1877.791 kb on + strand, within yeaR at 1877.804 kb on - strand, within yeaR at 1877.804 kb on - strand, within yeaR at 1877.804 kb on - strand, within yeaR at 1877.814 kb on - strand, within yeaR at 1877.859 kb on - strand, within yeaR at 1877.985 kb on + strand at 1878.018 kb on - strand at 1878.085 kb on - strand at 1878.142 kb on - strand at 1878.142 kb on - strand at 1878.186 kb on + strand at 1878.236 kb on + strand, within yeaS at 1878.236 kb on + strand, within yeaS at 1878.236 kb on + strand, within yeaS at 1878.236 kb on + strand, within yeaS at 1878.244 kb on - strand, within yeaS at 1878.244 kb on - strand, within yeaS at 1878.244 kb on - strand, within yeaS at 1878.244 kb on - strand, within yeaS at 1878.303 kb on - strand, within yeaS at 1878.340 kb on + strand, within yeaS at 1878.363 kb on + strand, within yeaS at 1878.363 kb on + strand, within yeaS at 1878.364 kb on - strand, within yeaS at 1878.388 kb on - strand, within yeaS at 1878.414 kb on - strand, within yeaS at 1878.446 kb on + strand, within yeaS at 1878.454 kb on - strand, within yeaS at 1878.484 kb on + strand, within yeaS at 1878.484 kb on + strand, within yeaS at 1878.492 kb on - strand, within yeaS at 1878.492 kb on - strand, within yeaS at 1878.492 kb on - strand, within yeaS at 1878.511 kb on + strand, within yeaS at 1878.598 kb on - strand, within yeaS at 1878.598 kb on - strand, within yeaS at 1878.598 kb on - strand, within yeaS at 1878.598 kb on - strand, within yeaS at 1878.608 kb on - strand, within yeaS
Per-strain Table
Position Strand Gene LocusTag Fraction capsaicin 2 mM remove 1,876,452 + yeaP b1794 0.69 -0.3 1,876,480 + yeaP b1794 0.72 +0.1 1,876,506 + yeaP b1794 0.75 +0.6 1,876,616 + yeaP b1794 0.85 -0.3 1,876,639 + yeaP b1794 0.88 -0.3 1,876,639 + yeaP b1794 0.88 +0.3 1,876,668 + -0.7 1,876,678 - -0.3 1,876,707 + -1.7 1,876,753 + -0.2 1,876,753 + +1.0 1,876,753 + +0.2 1,876,770 + -0.5 1,876,879 - yoaK b4676 0.83 -0.1 1,876,879 - yoaK b4676 0.83 +0.9 1,876,889 - -0.1 1,876,889 - +0.8 1,876,945 + yoaJ b4675 0.63 -0.4 1,876,945 + yoaJ b4675 0.63 -0.1 1,876,955 + yoaJ b4675 0.76 +0.1 1,876,963 - yoaJ b4675 0.87 +0.5 1,876,963 - yoaJ b4675 0.87 +0.7 1,877,005 + +0.3 1,877,014 - +0.5 1,877,035 + +0.2 1,877,135 - yeaQ b1795 0.42 -0.2 1,877,137 + yeaQ b1795 0.43 +0.7 1,877,145 - yeaQ b1795 0.46 +0.9 1,877,145 - yeaQ b1795 0.46 +0.1 1,877,174 - yeaQ b1795 0.57 +0.1 1,877,175 - yeaQ b1795 0.58 -0.4 1,877,260 + -0.6 1,877,433 + +0.5 1,877,476 + yoaG b1796 0.27 -0.4 1,877,476 + yoaG b1796 0.27 +0.1 1,877,484 - yoaG b1796 0.31 -0.4 1,877,513 - yoaG b1796 0.47 +0.1 1,877,571 - yoaG b1796 0.79 +0.5 1,877,602 - +0.4 1,877,658 - yeaR b1797 0.12 +0.2 1,877,663 + yeaR b1797 0.14 -0.9 1,877,663 + yeaR b1797 0.14 +0.3 1,877,714 + yeaR b1797 0.28 -0.1 1,877,715 - yeaR b1797 0.28 +0.7 1,877,764 + yeaR b1797 0.42 -0.5 1,877,765 + yeaR b1797 0.42 -0.0 1,877,765 + yeaR b1797 0.42 +0.6 1,877,791 + yeaR b1797 0.49 -1.0 1,877,804 - yeaR b1797 0.53 -0.1 1,877,804 - yeaR b1797 0.53 +0.3 1,877,804 - yeaR b1797 0.53 -0.2 1,877,814 - yeaR b1797 0.56 +1.0 1,877,859 - yeaR b1797 0.68 +0.1 1,877,985 + +0.7 1,878,018 - +0.7 1,878,085 - +1.1 1,878,142 - -0.2 1,878,142 - +0.7 1,878,186 + +0.3 1,878,236 + yeaS b1798 0.14 +0.7 1,878,236 + yeaS b1798 0.14 +0.1 1,878,236 + yeaS b1798 0.14 +0.4 1,878,236 + yeaS b1798 0.14 +0.1 1,878,244 - yeaS b1798 0.15 +0.4 1,878,244 - yeaS b1798 0.15 -0.2 1,878,244 - yeaS b1798 0.15 +1.3 1,878,244 - yeaS b1798 0.15 +0.4 1,878,303 - yeaS b1798 0.25 +0.5 1,878,340 + yeaS b1798 0.31 +0.3 1,878,363 + yeaS b1798 0.34 +0.6 1,878,363 + yeaS b1798 0.34 +0.5 1,878,364 - yeaS b1798 0.34 -0.3 1,878,388 - yeaS b1798 0.38 +0.1 1,878,414 - yeaS b1798 0.42 +0.2 1,878,446 + yeaS b1798 0.47 +0.3 1,878,454 - yeaS b1798 0.48 +0.3 1,878,484 + yeaS b1798 0.53 +0.2 1,878,484 + yeaS b1798 0.53 +0.7 1,878,492 - yeaS b1798 0.54 +0.2 1,878,492 - yeaS b1798 0.54 -0.9 1,878,492 - yeaS b1798 0.54 +0.5 1,878,511 + yeaS b1798 0.57 -1.4 1,878,598 - yeaS b1798 0.71 +0.4 1,878,598 - yeaS b1798 0.71 +0.1 1,878,598 - yeaS b1798 0.71 +0.1 1,878,598 - yeaS b1798 0.71 -0.0 1,878,608 - yeaS b1798 0.72 +0.0
Or see this region's nucleotide sequence