Experiment: LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF609 and GFF610 are separated by 18 nucleotides GFF610 and GFF611 are separated by 436 nucleotides GFF611 and GFF612 are separated by 98 nucleotides GFF612 and GFF613 are separated by 225 nucleotides
GFF609 - Ferrous iron transport protein B, at 346,625 to 348,943
GFF609
GFF610 - Ferrous iron transport protein A, at 348,962 to 349,189
GFF610
GFF611 - Transcription accessory protein (S1 RNA-binding domain), at 349,626 to 351,956
GFF611
GFF612 - Transcription elongation factor GreB, at 352,055 to 352,528
GFF612
GFF613 - Two-component system response regulator OmpR, at 352,754 to 353,473
GFF613
Position (kb) 349
350
351
352 Strain fitness (log2 ratio) -1
0
1 at 348.637 kb on - strand, within GFF609 at 348.763 kb on - strand at 348.823 kb on + strand at 348.884 kb on - strand at 348.888 kb on - strand at 348.888 kb on - strand at 348.888 kb on - strand at 348.963 kb on + strand at 348.987 kb on - strand, within GFF610 at 349.006 kb on + strand, within GFF610 at 349.079 kb on - strand, within GFF610 at 349.190 kb on - strand at 349.196 kb on + strand at 349.196 kb on + strand at 349.197 kb on - strand at 349.207 kb on + strand at 349.207 kb on + strand at 349.220 kb on + strand at 349.220 kb on + strand at 349.220 kb on + strand at 349.221 kb on - strand at 349.221 kb on - strand at 349.222 kb on + strand at 349.265 kb on + strand at 349.814 kb on + strand at 349.815 kb on - strand at 349.821 kb on - strand at 349.821 kb on - strand at 349.821 kb on - strand at 350.132 kb on + strand, within GFF611 at 350.196 kb on + strand, within GFF611 at 350.289 kb on - strand, within GFF611 at 350.292 kb on - strand, within GFF611 at 350.754 kb on + strand, within GFF611 at 350.755 kb on - strand, within GFF611 at 350.764 kb on + strand, within GFF611 at 350.764 kb on + strand, within GFF611 at 350.793 kb on - strand, within GFF611 at 351.199 kb on + strand, within GFF611 at 351.200 kb on - strand, within GFF611 at 351.308 kb on + strand, within GFF611 at 351.409 kb on + strand, within GFF611 at 351.410 kb on - strand, within GFF611 at 351.417 kb on + strand, within GFF611 at 351.669 kb on - strand, within GFF611 at 351.929 kb on + strand at 351.961 kb on + strand at 351.962 kb on - strand at 352.066 kb on - strand at 352.186 kb on + strand, within GFF612 at 352.187 kb on - strand, within GFF612 at 352.375 kb on + strand, within GFF612 at 352.379 kb on - strand, within GFF612 at 352.381 kb on - strand, within GFF612 at 352.470 kb on + strand, within GFF612 at 352.471 kb on - strand, within GFF612 at 352.540 kb on - strand at 352.571 kb on - strand at 352.572 kb on + strand at 352.599 kb on - strand at 352.713 kb on + strand at 352.714 kb on - strand at 352.721 kb on + strand at 352.721 kb on + strand at 352.721 kb on + strand at 352.747 kb on - strand at 352.792 kb on + strand at 352.912 kb on - strand, within GFF613 at 352.912 kb on - strand, within GFF613 at 352.921 kb on - strand, within GFF613
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI remove 348,637 - GFF609 0.87 -0.2 348,763 - +0.3 348,823 + +0.1 348,884 - +0.2 348,888 - +0.7 348,888 - +0.8 348,888 - +0.4 348,963 + -0.3 348,987 - GFF610 0.11 -0.3 349,006 + GFF610 0.19 -0.0 349,079 - GFF610 0.51 +0.6 349,190 - -0.0 349,196 + +0.3 349,196 + +0.3 349,197 - +0.3 349,207 + +1.4 349,207 + -0.1 349,220 + +0.2 349,220 + -1.3 349,220 + -0.2 349,221 - -0.5 349,221 - -0.1 349,222 + -0.2 349,265 + -0.2 349,814 + -0.5 349,815 - +0.3 349,821 - -0.1 349,821 - -0.3 349,821 - +0.0 350,132 + GFF611 0.22 +0.2 350,196 + GFF611 0.24 -0.9 350,289 - GFF611 0.28 +0.7 350,292 - GFF611 0.29 +0.0 350,754 + GFF611 0.48 +0.6 350,755 - GFF611 0.48 +1.1 350,764 + GFF611 0.49 -0.1 350,764 + GFF611 0.49 -0.1 350,793 - GFF611 0.50 -0.2 351,199 + GFF611 0.67 +0.7 351,200 - GFF611 0.68 -0.5 351,308 + GFF611 0.72 +0.9 351,409 + GFF611 0.76 +0.6 351,410 - GFF611 0.77 -0.1 351,417 + GFF611 0.77 +0.5 351,669 - GFF611 0.88 -0.0 351,929 + +0.2 351,961 + -0.6 351,962 - +0.4 352,066 - +0.2 352,186 + GFF612 0.28 +0.0 352,187 - GFF612 0.28 -1.0 352,375 + GFF612 0.68 -0.4 352,379 - GFF612 0.68 -0.2 352,381 - GFF612 0.69 -0.3 352,470 + GFF612 0.88 +0.1 352,471 - GFF612 0.88 -0.5 352,540 - -0.1 352,571 - -0.0 352,572 + -0.1 352,599 - +0.7 352,713 + +0.0 352,714 - +0.3 352,721 + +0.2 352,721 + +0.1 352,721 + -0.8 352,747 - +0.1 352,792 + +0.4 352,912 - GFF613 0.22 +0.5 352,912 - GFF613 0.22 +0.2 352,921 - GFF613 0.23 +0.1
Or see this region's nucleotide sequence