Experiment: LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4058 and GFF4059 are separated by 19 nucleotides GFF4059 and GFF4060 are separated by 129 nucleotides GFF4060 and GFF4061 are separated by 35 nucleotides GFF4061 and GFF4062 are separated by 142 nucleotides GFF4062 and GFF4063 are separated by 111 nucleotides
GFF4058 - Putative cytoplasmic protein USSDB7A, at 22,303 to 22,788
GFF4058
GFF4059 - Type VI secretion lipoprotein/VasD, at 22,808 to 22,963
GFF4059
GFF4060 - putative cytoplasmic protein, at 23,093 to 23,578
GFF4060
GFF4061 - putative periplasmic protein, at 23,614 to 23,946
GFF4061
GFF4062 - Putative cytoplasmic protein USSDB7A, at 24,089 to 24,574
GFF4062
GFF4063 - Type VI secretion lipoprotein/VasD, at 24,686 to 25,177
GFF4063
Position (kb) 23
24 Strain fitness (log2 ratio) -1
0
1 at 22.950 kb on + strand at 22.997 kb on + strand at 23.044 kb on - strand at 23.065 kb on + strand at 23.318 kb on + strand, within GFF4060 at 23.663 kb on - strand, within GFF4061 at 24.269 kb on + strand, within GFF4062 at 24.270 kb on - strand, within GFF4062 at 24.270 kb on - strand, within GFF4062 at 24.302 kb on + strand, within GFF4062 at 24.829 kb on - strand, within GFF4063
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI remove 22,950 + -0.2 22,997 + -0.5 23,044 - +0.6 23,065 + +0.4 23,318 + GFF4060 0.46 -0.7 23,663 - GFF4061 0.15 +0.2 24,269 + GFF4062 0.37 +0.2 24,270 - GFF4062 0.37 +0.4 24,270 - GFF4062 0.37 -0.1 24,302 + GFF4062 0.44 -0.3 24,829 - GFF4063 0.29 +1.1
Or see this region's nucleotide sequence