Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4049

Experiment: LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4048 and GFF4049 are separated by 16 nucleotidesGFF4049 and GFF4050 overlap by 4 nucleotides GFF4048 - Uncharacterized protein ImpA, at 10,075 to 11,124 GFF4048 GFF4049 - Uncharacterized protein ImpH/VasB, at 11,141 to 12,136 GFF4049 GFF4050 - Protein ImpG/VasA, at 12,133 to 14,016 GFF4050 Position (kb) 11 12 13Strain fitness (log2 ratio) -1 0 1at 10.417 kb on + strand, within GFF4048at 10.686 kb on + strand, within GFF4048at 10.686 kb on + strand, within GFF4048at 10.927 kb on - strand, within GFF4048at 11.012 kb on + strand, within GFF4048at 11.137 kb on - strandat 11.320 kb on + strand, within GFF4049at 11.402 kb on - strand, within GFF4049at 11.555 kb on + strand, within GFF4049at 11.566 kb on - strand, within GFF4049at 11.703 kb on + strand, within GFF4049at 11.916 kb on + strand, within GFF4049at 11.917 kb on - strand, within GFF4049at 12.047 kb on - strandat 12.219 kb on + strandat 12.243 kb on + strandat 12.245 kb on + strandat 12.246 kb on - strandat 12.246 kb on - strandat 12.273 kb on - strandat 12.335 kb on + strand, within GFF4050at 12.622 kb on + strand, within GFF4050at 12.623 kb on - strand, within GFF4050at 12.654 kb on + strand, within GFF4050at 12.954 kb on - strand, within GFF4050at 13.126 kb on + strand, within GFF4050at 13.127 kb on - strand, within GFF4050

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI
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10,417 + GFF4048 0.33 +0.2
10,686 + GFF4048 0.58 +0.6
10,686 + GFF4048 0.58 -1.7
10,927 - GFF4048 0.81 -0.4
11,012 + GFF4048 0.89 +0.1
11,137 - -0.3
11,320 + GFF4049 0.18 +0.2
11,402 - GFF4049 0.26 +0.9
11,555 + GFF4049 0.42 +1.0
11,566 - GFF4049 0.43 +0.1
11,703 + GFF4049 0.56 -0.1
11,916 + GFF4049 0.78 +0.0
11,917 - GFF4049 0.78 +0.0
12,047 - +0.2
12,219 + +0.7
12,243 + +0.2
12,245 + +0.5
12,246 - -0.2
12,246 - +0.4
12,273 - +0.5
12,335 + GFF4050 0.11 -0.0
12,622 + GFF4050 0.26 -0.1
12,623 - GFF4050 0.26 -0.7
12,654 + GFF4050 0.28 -0.5
12,954 - GFF4050 0.44 +0.5
13,126 + GFF4050 0.53 +1.7
13,127 - GFF4050 0.53 -0.0

Or see this region's nucleotide sequence