Experiment: LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3811 and GFF3812 are separated by 57 nucleotides GFF3812 and GFF3813 are separated by 296 nucleotides
GFF3811 - Murein-DD-endopeptidase (EC 3.4.99.-), at 78,419 to 79,327
GFF3811
GFF3812 - D-Lactate dehydrogenase (EC 1.1.2.5), at 79,385 to 81,115
GFF3812
GFF3813 - Periplasmic beta-glucosidase (EC 3.2.1.21), at 81,412 to 83,679
GFF3813
Position (kb)
79
80
81
82 Strain fitness (log2 ratio)
-1
0
1 at 78.684 kb on - strand, within GFF3811 at 78.724 kb on + strand, within GFF3811 at 78.809 kb on - strand, within GFF3811 at 78.854 kb on - strand, within GFF3811 at 78.960 kb on + strand, within GFF3811 at 78.961 kb on - strand, within GFF3811 at 79.041 kb on - strand, within GFF3811 at 79.169 kb on + strand, within GFF3811 at 79.172 kb on - strand, within GFF3811 at 79.201 kb on + strand, within GFF3811 at 79.202 kb on - strand, within GFF3811 at 79.619 kb on - strand, within GFF3812 at 79.621 kb on - strand, within GFF3812 at 79.621 kb on - strand, within GFF3812 at 79.646 kb on - strand, within GFF3812 at 79.656 kb on + strand, within GFF3812 at 79.666 kb on + strand, within GFF3812 at 80.189 kb on - strand, within GFF3812 at 80.277 kb on + strand, within GFF3812 at 80.389 kb on - strand, within GFF3812 at 80.414 kb on - strand, within GFF3812 at 80.822 kb on - strand, within GFF3812 at 81.089 kb on - strand at 81.089 kb on - strand at 81.091 kb on + strand at 81.251 kb on + strand at 81.393 kb on - strand at 81.542 kb on + strand at 81.543 kb on - strand at 81.709 kb on + strand, within GFF3813 at 81.794 kb on + strand, within GFF3813 at 81.794 kb on + strand, within GFF3813 at 81.916 kb on + strand, within GFF3813 at 81.917 kb on - strand, within GFF3813 at 81.920 kb on + strand, within GFF3813 at 82.087 kb on + strand, within GFF3813 at 82.088 kb on - strand, within GFF3813
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI remove 78,684 - GFF3811 0.29 +0.1 78,724 + GFF3811 0.34 +0.0 78,809 - GFF3811 0.43 -0.8 78,854 - GFF3811 0.48 -0.3 78,960 + GFF3811 0.60 +0.7 78,961 - GFF3811 0.60 -0.2 79,041 - GFF3811 0.68 +0.5 79,169 + GFF3811 0.83 -0.1 79,172 - GFF3811 0.83 -0.2 79,201 + GFF3811 0.86 +0.0 79,202 - GFF3811 0.86 +0.0 79,619 - GFF3812 0.14 -0.5 79,621 - GFF3812 0.14 -0.0 79,621 - GFF3812 0.14 -0.3 79,646 - GFF3812 0.15 -0.4 79,656 + GFF3812 0.16 -0.5 79,666 + GFF3812 0.16 +0.1 80,189 - GFF3812 0.46 -0.7 80,277 + GFF3812 0.52 +0.5 80,389 - GFF3812 0.58 -0.3 80,414 - GFF3812 0.59 -0.8 80,822 - GFF3812 0.83 +0.2 81,089 - -0.2 81,089 - -0.0 81,091 + -0.5 81,251 + +0.4 81,393 - -0.4 81,542 + -0.0 81,543 - -0.2 81,709 + GFF3813 0.13 -0.3 81,794 + GFF3813 0.17 -0.1 81,794 + GFF3813 0.17 -0.2 81,916 + GFF3813 0.22 -0.1 81,917 - GFF3813 0.22 -0.1 81,920 + GFF3813 0.22 +1.5 82,087 + GFF3813 0.30 -1.5 82,088 - GFF3813 0.30 -0.2
Or see this region's nucleotide sequence