Experiment: LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3615 and GFF3616 are separated by 289 nucleotides GFF3616 and GFF3617 are separated by 112 nucleotides
GFF3615 - Cyclopropane-fatty-acyl-phospholipid synthase (EC 2.1.1.79), at 366,288 to 367,436
GFF3615
GFF3616 - Putative transport protein, at 367,726 to 368,931
GFF3616
GFF3617 - LysR-family transcriptional regulator YdhB, at 369,044 to 369,976
GFF3617
Position (kb) 367
368
369 Strain fitness (log2 ratio) -1
0
1 at 366.885 kb on - strand, within GFF3615 at 366.885 kb on - strand, within GFF3615 at 366.886 kb on + strand, within GFF3615 at 366.943 kb on - strand, within GFF3615 at 366.943 kb on - strand, within GFF3615 at 367.027 kb on + strand, within GFF3615 at 367.035 kb on - strand, within GFF3615 at 367.049 kb on + strand, within GFF3615 at 367.078 kb on + strand, within GFF3615 at 367.119 kb on + strand, within GFF3615 at 367.314 kb on - strand, within GFF3615 at 367.349 kb on + strand at 367.622 kb on - strand at 367.636 kb on + strand at 367.808 kb on - strand at 367.921 kb on - strand, within GFF3616 at 367.967 kb on - strand, within GFF3616 at 368.099 kb on + strand, within GFF3616 at 368.100 kb on - strand, within GFF3616 at 368.107 kb on + strand, within GFF3616 at 368.107 kb on + strand, within GFF3616 at 368.108 kb on - strand, within GFF3616 at 368.201 kb on + strand, within GFF3616 at 368.201 kb on + strand, within GFF3616 at 368.332 kb on + strand, within GFF3616 at 368.356 kb on - strand, within GFF3616 at 368.356 kb on - strand, within GFF3616 at 368.424 kb on + strand, within GFF3616 at 368.695 kb on - strand, within GFF3616 at 368.755 kb on + strand, within GFF3616 at 368.755 kb on + strand, within GFF3616 at 368.858 kb on + strand at 368.858 kb on + strand at 368.859 kb on - strand at 368.859 kb on - strand at 368.909 kb on - strand at 368.932 kb on - strand at 369.193 kb on - strand, within GFF3617 at 369.324 kb on + strand, within GFF3617 at 369.328 kb on + strand, within GFF3617 at 369.329 kb on - strand, within GFF3617 at 369.903 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI remove 366,885 - GFF3615 0.52 +0.7 366,885 - GFF3615 0.52 -1.0 366,886 + GFF3615 0.52 +0.1 366,943 - GFF3615 0.57 +0.7 366,943 - GFF3615 0.57 +0.2 367,027 + GFF3615 0.64 +0.4 367,035 - GFF3615 0.65 +0.5 367,049 + GFF3615 0.66 -1.0 367,078 + GFF3615 0.69 -0.3 367,119 + GFF3615 0.72 +0.1 367,314 - GFF3615 0.89 -0.5 367,349 + -0.4 367,622 - +0.0 367,636 + -0.2 367,808 - -0.2 367,921 - GFF3616 0.16 -0.2 367,967 - GFF3616 0.20 +0.1 368,099 + GFF3616 0.31 -0.2 368,100 - GFF3616 0.31 -0.2 368,107 + GFF3616 0.32 +0.5 368,107 + GFF3616 0.32 -0.5 368,108 - GFF3616 0.32 -0.6 368,201 + GFF3616 0.39 -0.5 368,201 + GFF3616 0.39 -0.7 368,332 + GFF3616 0.50 -0.0 368,356 - GFF3616 0.52 -0.3 368,356 - GFF3616 0.52 -0.1 368,424 + GFF3616 0.58 -0.1 368,695 - GFF3616 0.80 +0.5 368,755 + GFF3616 0.85 -0.2 368,755 + GFF3616 0.85 -0.5 368,858 + -0.3 368,858 + -0.0 368,859 - +0.2 368,859 - -0.5 368,909 - +0.5 368,932 - -0.1 369,193 - GFF3617 0.16 -0.0 369,324 + GFF3617 0.30 +0.2 369,328 + GFF3617 0.30 -0.3 369,329 - GFF3617 0.31 -0.6 369,903 - -0.2
Or see this region's nucleotide sequence