Experiment: LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3130 and GFF3131 are separated by 41 nucleotides GFF3131 and GFF3132 are separated by 10 nucleotides
GFF3130 - TRAP-type C4-dicarboxylate transport system, periplasmic component, at 136,732 to 137,715
GFF3130
GFF3131 - TRAP-type C4-dicarboxylate transport system, small permease component, at 137,757 to 138,239
GFF3131
GFF3132 - TRAP-type C4-dicarboxylate transport system, large permease component, at 138,250 to 139,557
GFF3132
Position (kb) 137
138
139 Strain fitness (log2 ratio) -1
0
1 at 136.873 kb on + strand, within GFF3130 at 136.873 kb on + strand, within GFF3130 at 136.874 kb on - strand, within GFF3130 at 136.917 kb on + strand, within GFF3130 at 137.039 kb on - strand, within GFF3130 at 137.041 kb on - strand, within GFF3130 at 137.087 kb on - strand, within GFF3130 at 137.093 kb on + strand, within GFF3130 at 137.374 kb on + strand, within GFF3130 at 137.506 kb on - strand, within GFF3130 at 137.572 kb on + strand, within GFF3130 at 137.573 kb on - strand, within GFF3130 at 137.698 kb on + strand at 137.710 kb on - strand at 137.714 kb on - strand at 137.753 kb on + strand at 137.753 kb on + strand at 137.753 kb on + strand at 137.808 kb on - strand, within GFF3131 at 138.038 kb on + strand, within GFF3131 at 138.216 kb on + strand at 138.248 kb on - strand at 138.264 kb on + strand at 138.305 kb on + strand at 138.324 kb on + strand at 138.368 kb on + strand at 138.648 kb on - strand, within GFF3132 at 138.851 kb on - strand, within GFF3132 at 138.868 kb on + strand, within GFF3132 at 138.945 kb on - strand, within GFF3132 at 139.016 kb on - strand, within GFF3132 at 139.101 kb on + strand, within GFF3132
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI remove 136,873 + GFF3130 0.14 +1.2 136,873 + GFF3130 0.14 -0.0 136,874 - GFF3130 0.14 -0.4 136,917 + GFF3130 0.19 +1.2 137,039 - GFF3130 0.31 +0.4 137,041 - GFF3130 0.31 +0.2 137,087 - GFF3130 0.36 +0.1 137,093 + GFF3130 0.37 -0.2 137,374 + GFF3130 0.65 -0.1 137,506 - GFF3130 0.79 +0.5 137,572 + GFF3130 0.85 -0.9 137,573 - GFF3130 0.85 +0.1 137,698 + +0.1 137,710 - -0.2 137,714 - +0.4 137,753 + -0.1 137,753 + +0.6 137,753 + -1.2 137,808 - GFF3131 0.11 -0.2 138,038 + GFF3131 0.58 -1.2 138,216 + +0.7 138,248 - +0.8 138,264 + +0.1 138,305 + +0.2 138,324 + +1.3 138,368 + +0.4 138,648 - GFF3132 0.30 +0.2 138,851 - GFF3132 0.46 +0.5 138,868 + GFF3132 0.47 -0.5 138,945 - GFF3132 0.53 +0.3 139,016 - GFF3132 0.59 +0.1 139,101 + GFF3132 0.65 +0.0
Or see this region's nucleotide sequence