Experiment: LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2723 and GFF2724 are separated by 3 nucleotides GFF2724 and GFF2725 are separated by 9 nucleotides
GFF2723 - Anthranilate synthase, amidotransferase component (EC 4.1.3.27) / Anthranilate phosphoribosyltransferase (EC 2.4.2.18), at 245,730 to 247,325
GFF2723
GFF2724 - Indole-3-glycerol phosphate synthase (EC 4.1.1.48) / Phosphoribosylanthranilate isomerase (EC 5.3.1.24), at 247,329 to 248,687
GFF2724
GFF2725 - Tryptophan synthase beta chain (EC 4.2.1.20), at 248,697 to 249,890
GFF2725
Position (kb) 247
248
249 Strain fitness (log2 ratio) -1
0
1 at 246.541 kb on - strand, within GFF2723 at 246.581 kb on + strand, within GFF2723 at 246.582 kb on - strand, within GFF2723 at 246.594 kb on - strand, within GFF2723 at 246.710 kb on + strand, within GFF2723 at 246.787 kb on - strand, within GFF2723 at 246.976 kb on + strand, within GFF2723 at 247.049 kb on + strand, within GFF2723 at 247.153 kb on + strand, within GFF2723 at 247.324 kb on - strand at 247.856 kb on - strand, within GFF2724 at 247.979 kb on + strand, within GFF2724 at 247.986 kb on + strand, within GFF2724 at 247.987 kb on - strand, within GFF2724 at 247.987 kb on - strand, within GFF2724 at 247.987 kb on - strand, within GFF2724 at 247.987 kb on - strand, within GFF2724 at 248.064 kb on - strand, within GFF2724 at 248.158 kb on + strand, within GFF2724 at 248.158 kb on + strand, within GFF2724 at 248.158 kb on + strand, within GFF2724 at 248.160 kb on + strand, within GFF2724 at 248.326 kb on + strand, within GFF2724 at 248.350 kb on - strand, within GFF2724 at 248.350 kb on - strand, within GFF2724 at 249.093 kb on + strand, within GFF2725 at 249.094 kb on - strand, within GFF2725 at 249.094 kb on - strand, within GFF2725 at 249.094 kb on - strand, within GFF2725 at 249.252 kb on + strand, within GFF2725 at 249.252 kb on + strand, within GFF2725 at 249.253 kb on - strand, within GFF2725 at 249.531 kb on + strand, within GFF2725 at 249.532 kb on - strand, within GFF2725 at 249.666 kb on + strand, within GFF2725 at 249.667 kb on - strand, within GFF2725
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI remove 246,541 - GFF2723 0.51 +0.3 246,581 + GFF2723 0.53 -0.2 246,582 - GFF2723 0.53 -0.2 246,594 - GFF2723 0.54 -0.5 246,710 + GFF2723 0.61 -0.2 246,787 - GFF2723 0.66 -0.4 246,976 + GFF2723 0.78 +0.6 247,049 + GFF2723 0.83 +0.8 247,153 + GFF2723 0.89 +0.1 247,324 - +0.6 247,856 - GFF2724 0.39 +0.1 247,979 + GFF2724 0.48 -0.2 247,986 + GFF2724 0.48 -0.2 247,987 - GFF2724 0.48 -0.6 247,987 - GFF2724 0.48 -1.5 247,987 - GFF2724 0.48 +0.3 247,987 - GFF2724 0.48 -0.1 248,064 - GFF2724 0.54 -0.8 248,158 + GFF2724 0.61 +0.0 248,158 + GFF2724 0.61 -0.2 248,158 + GFF2724 0.61 -0.4 248,160 + GFF2724 0.61 +0.0 248,326 + GFF2724 0.73 -0.5 248,350 - GFF2724 0.75 -0.6 248,350 - GFF2724 0.75 -0.2 249,093 + GFF2725 0.33 -0.1 249,094 - GFF2725 0.33 -0.4 249,094 - GFF2725 0.33 +1.0 249,094 - GFF2725 0.33 -0.7 249,252 + GFF2725 0.46 +0.2 249,252 + GFF2725 0.46 -0.0 249,253 - GFF2725 0.47 +1.0 249,531 + GFF2725 0.70 -0.3 249,532 - GFF2725 0.70 +1.0 249,666 + GFF2725 0.81 +0.4 249,667 - GFF2725 0.81 +0.3
Or see this region's nucleotide sequence