Experiment: LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2334 and GFF2335 are separated by 320 nucleotides GFF2335 and GFF2336 are separated by 14 nucleotides GFF2336 and GFF2337 are separated by 98 nucleotides
GFF2334 - Phosphoenolpyruvate-protein phosphotransferase of PTS system (EC 2.7.3.9), at 123,415 to 125,904
GFF2334
GFF2335 - PTS system, fructose-specific IIC component, at 126,225 to 127,304
GFF2335
GFF2336 - PTS system fructose-like IIB component 1 precursor (EC 2.7.1.69), at 127,319 to 127,639
GFF2336
GFF2337 - Pyruvate formate-lyase (EC 2.3.1.54), at 127,738 to 130,035
GFF2337
Position (kb)
126
127
128 Strain fitness (log2 ratio)
-2
-1
0
1 at 125.291 kb on - strand, within GFF2334 at 125.315 kb on - strand, within GFF2334 at 125.409 kb on + strand, within GFF2334 at 125.429 kb on - strand, within GFF2334 at 125.545 kb on - strand, within GFF2334 at 125.832 kb on + strand at 125.836 kb on - strand at 125.848 kb on + strand at 125.872 kb on + strand at 125.873 kb on - strand at 125.873 kb on - strand at 125.895 kb on + strand at 125.953 kb on - strand at 126.287 kb on + strand at 126.288 kb on - strand at 126.340 kb on + strand, within GFF2335 at 126.340 kb on + strand, within GFF2335 at 126.341 kb on - strand, within GFF2335 at 126.341 kb on - strand, within GFF2335 at 126.447 kb on + strand, within GFF2335 at 126.447 kb on + strand, within GFF2335 at 126.457 kb on - strand, within GFF2335 at 126.580 kb on - strand, within GFF2335 at 126.657 kb on - strand, within GFF2335 at 126.860 kb on - strand, within GFF2335 at 126.862 kb on - strand, within GFF2335 at 126.862 kb on - strand, within GFF2335 at 126.863 kb on + strand, within GFF2335 at 126.863 kb on + strand, within GFF2335 at 126.864 kb on - strand, within GFF2335 at 127.062 kb on - strand, within GFF2335 at 127.117 kb on - strand, within GFF2335 at 127.302 kb on + strand at 127.370 kb on + strand, within GFF2336 at 127.370 kb on + strand, within GFF2336 at 127.370 kb on + strand, within GFF2336 at 127.370 kb on + strand, within GFF2336 at 127.370 kb on + strand, within GFF2336 at 127.370 kb on + strand, within GFF2336 at 127.370 kb on + strand, within GFF2336 at 127.370 kb on + strand, within GFF2336 at 127.371 kb on - strand, within GFF2336 at 127.371 kb on - strand, within GFF2336 at 127.422 kb on + strand, within GFF2336 at 127.513 kb on + strand, within GFF2336 at 127.547 kb on - strand, within GFF2336 at 127.552 kb on + strand, within GFF2336 at 127.600 kb on + strand, within GFF2336 at 127.749 kb on + strand at 127.816 kb on + strand at 127.882 kb on + strand at 127.966 kb on - strand at 128.119 kb on - strand, within GFF2337 at 128.121 kb on + strand, within GFF2337
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI remove 125,291 - GFF2334 0.75 -0.7 125,315 - GFF2334 0.76 +0.5 125,409 + GFF2334 0.80 -1.8 125,429 - GFF2334 0.81 -0.2 125,545 - GFF2334 0.86 +0.7 125,832 + -1.1 125,836 - -1.1 125,848 + +0.2 125,872 + +0.1 125,873 - -0.1 125,873 - -0.2 125,895 + +0.6 125,953 - +0.8 126,287 + +0.2 126,288 - -0.0 126,340 + GFF2335 0.11 -0.0 126,340 + GFF2335 0.11 +1.5 126,341 - GFF2335 0.11 -0.5 126,341 - GFF2335 0.11 +0.0 126,447 + GFF2335 0.21 -0.6 126,447 + GFF2335 0.21 -0.2 126,457 - GFF2335 0.21 -0.0 126,580 - GFF2335 0.33 +0.1 126,657 - GFF2335 0.40 +0.9 126,860 - GFF2335 0.59 -0.3 126,862 - GFF2335 0.59 +0.2 126,862 - GFF2335 0.59 -0.5 126,863 + GFF2335 0.59 +0.7 126,863 + GFF2335 0.59 +0.4 126,864 - GFF2335 0.59 +0.1 127,062 - GFF2335 0.78 +1.2 127,117 - GFF2335 0.83 -0.1 127,302 + +0.3 127,370 + GFF2336 0.16 +0.5 127,370 + GFF2336 0.16 +0.2 127,370 + GFF2336 0.16 -1.1 127,370 + GFF2336 0.16 -0.2 127,370 + GFF2336 0.16 -0.0 127,370 + GFF2336 0.16 +1.6 127,370 + GFF2336 0.16 -0.3 127,370 + GFF2336 0.16 +0.6 127,371 - GFF2336 0.16 -0.4 127,371 - GFF2336 0.16 -0.5 127,422 + GFF2336 0.32 -0.5 127,513 + GFF2336 0.60 +0.2 127,547 - GFF2336 0.71 +0.0 127,552 + GFF2336 0.73 -0.1 127,600 + GFF2336 0.88 +0.0 127,749 + +0.3 127,816 + +0.9 127,882 + +0.4 127,966 - +1.6 128,119 - GFF2337 0.17 +0.6 128,121 + GFF2337 0.17 -0.7
Or see this region's nucleotide sequence