Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1887

Experiment: LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1886 and GFF1887 are separated by 30 nucleotidesGFF1887 and GFF1888 overlap by 4 nucleotidesGFF1888 and GFF1889 are separated by 2 nucleotides GFF1886 - NADH-ubiquinone oxidoreductase chain G (EC 1.6.5.3), at 78,287 to 81,013 GFF1886 GFF1887 - NADH-ubiquinone oxidoreductase chain F (EC 1.6.5.3), at 81,044 to 82,381 GFF1887 GFF1888 - NADH-ubiquinone oxidoreductase chain E (EC 1.6.5.3), at 82,378 to 82,878 GFF1888 GFF1889 - NADH-ubiquinone oxidoreductase chain C (EC 1.6.5.3) / NADH-ubiquinone oxidoreductase chain D (EC 1.6.5.3), at 82,881 to 84,683 GFF1889 Position (kb) 81 82 83Strain fitness (log2 ratio) -3 -2 -1 0 1at 80.199 kb on + strand, within GFF1886at 80.199 kb on + strand, within GFF1886at 80.199 kb on + strand, within GFF1886at 80.200 kb on - strand, within GFF1886at 80.309 kb on - strand, within GFF1886at 80.476 kb on - strand, within GFF1886at 80.665 kb on + strand, within GFF1886at 80.786 kb on - strandat 80.791 kb on + strandat 80.919 kb on + strandat 80.927 kb on - strandat 80.927 kb on - strandat 80.927 kb on - strandat 80.955 kb on - strandat 81.145 kb on + strandat 81.145 kb on + strandat 81.319 kb on + strand, within GFF1887at 81.319 kb on + strand, within GFF1887at 81.960 kb on - strand, within GFF1887at 81.974 kb on - strand, within GFF1887at 81.996 kb on + strand, within GFF1887at 81.997 kb on - strand, within GFF1887at 81.997 kb on - strand, within GFF1887at 82.036 kb on + strand, within GFF1887at 82.036 kb on + strand, within GFF1887at 82.037 kb on - strand, within GFF1887at 82.086 kb on + strand, within GFF1887at 82.653 kb on - strand, within GFF1888at 82.653 kb on - strand, within GFF1888at 82.712 kb on + strand, within GFF1888at 82.813 kb on + strand, within GFF1888at 82.814 kb on - strand, within GFF1888at 82.882 kb on + strandat 82.901 kb on + strandat 83.021 kb on + strandat 83.022 kb on - strandat 83.045 kb on - strandat 83.047 kb on + strandat 83.226 kb on - strand, within GFF1889at 83.293 kb on + strand, within GFF1889at 83.293 kb on + strand, within GFF1889at 83.293 kb on + strand, within GFF1889at 83.293 kb on + strand, within GFF1889at 83.294 kb on - strand, within GFF1889at 83.366 kb on + strand, within GFF1889

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI
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80,199 + GFF1886 0.70 -1.4
80,199 + GFF1886 0.70 -0.2
80,199 + GFF1886 0.70 -2.5
80,200 - GFF1886 0.70 -1.5
80,309 - GFF1886 0.74 -1.0
80,476 - GFF1886 0.80 +0.6
80,665 + GFF1886 0.87 -1.6
80,786 - -1.8
80,791 + -1.0
80,919 + -0.6
80,927 - -1.4
80,927 - -1.3
80,927 - -1.9
80,955 - -0.9
81,145 + -1.7
81,145 + -1.7
81,319 + GFF1887 0.21 -0.4
81,319 + GFF1887 0.21 -2.1
81,960 - GFF1887 0.68 -0.3
81,974 - GFF1887 0.70 -1.7
81,996 + GFF1887 0.71 -1.4
81,997 - GFF1887 0.71 -1.9
81,997 - GFF1887 0.71 -1.4
82,036 + GFF1887 0.74 -1.7
82,036 + GFF1887 0.74 -2.4
82,037 - GFF1887 0.74 -2.2
82,086 + GFF1887 0.78 -0.7
82,653 - GFF1888 0.55 -1.1
82,653 - GFF1888 0.55 +0.5
82,712 + GFF1888 0.67 -1.6
82,813 + GFF1888 0.87 -0.4
82,814 - GFF1888 0.87 -0.7
82,882 + -1.6
82,901 + -0.8
83,021 + -1.3
83,022 - -2.2
83,045 - -1.1
83,047 + -3.0
83,226 - GFF1889 0.19 -1.4
83,293 + GFF1889 0.23 -1.3
83,293 + GFF1889 0.23 -1.0
83,293 + GFF1889 0.23 -0.4
83,293 + GFF1889 0.23 -1.3
83,294 - GFF1889 0.23 -0.6
83,366 + GFF1889 0.27 -1.6

Or see this region's nucleotide sequence