Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF1881

Experiment: LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF1880 and GFF1881 are separated by 313 nucleotidesGFF1881 and GFF1882 are separated by 2 nucleotidesGFF1882 and GFF1883 overlap by 4 nucleotidesGFF1883 and GFF1884 are separated by 10 nucleotides GFF1880 - NADH-ubiquinone oxidoreductase chain M (EC 1.6.5.3), at 72,211 to 73,740 GFF1880 GFF1881 - NADH-ubiquinone oxidoreductase chain L (EC 1.6.5.3), at 74,054 to 75,889 GFF1881 GFF1882 - NADH-ubiquinone oxidoreductase chain K (EC 1.6.5.3), at 75,892 to 76,194 GFF1882 GFF1883 - NADH-ubiquinone oxidoreductase chain J (EC 1.6.5.3), at 76,191 to 76,745 GFF1883 GFF1884 - NADH-ubiquinone oxidoreductase chain I (EC 1.6.5.3), at 76,756 to 77,298 GFF1884 Position (kb) 74 75 76Strain fitness (log2 ratio) -3 -2 -1 0 1at 73.212 kb on - strand, within GFF1880at 73.474 kb on + strand, within GFF1880at 73.506 kb on + strand, within GFF1880at 73.506 kb on + strand, within GFF1880at 73.736 kb on - strandat 73.745 kb on - strandat 73.887 kb on + strandat 73.887 kb on + strandat 73.888 kb on - strandat 73.888 kb on - strandat 73.896 kb on - strandat 74.011 kb on + strandat 74.115 kb on + strandat 74.116 kb on - strandat 74.116 kb on - strandat 74.116 kb on - strandat 74.128 kb on - strandat 74.252 kb on + strand, within GFF1881at 74.253 kb on - strand, within GFF1881at 74.263 kb on - strand, within GFF1881at 74.289 kb on + strand, within GFF1881at 74.290 kb on - strand, within GFF1881at 74.968 kb on - strand, within GFF1881at 75.021 kb on - strand, within GFF1881at 75.028 kb on + strand, within GFF1881at 75.028 kb on + strand, within GFF1881at 75.331 kb on - strand, within GFF1881at 75.331 kb on - strand, within GFF1881at 75.359 kb on + strand, within GFF1881at 75.427 kb on - strand, within GFF1881at 75.445 kb on - strand, within GFF1881at 75.478 kb on - strand, within GFF1881at 75.478 kb on - strand, within GFF1881at 75.538 kb on + strand, within GFF1881at 75.539 kb on - strand, within GFF1881at 75.541 kb on - strand, within GFF1881at 75.579 kb on + strand, within GFF1881at 75.872 kb on - strandat 75.911 kb on - strandat 76.010 kb on + strand, within GFF1882at 76.010 kb on + strand, within GFF1882at 76.010 kb on + strand, within GFF1882at 76.011 kb on - strand, within GFF1882at 76.011 kb on - strand, within GFF1882at 76.105 kb on - strand, within GFF1882at 76.153 kb on + strand, within GFF1882at 76.310 kb on + strand, within GFF1883at 76.311 kb on - strand, within GFF1883at 76.311 kb on - strand, within GFF1883at 76.336 kb on + strand, within GFF1883at 76.421 kb on - strand, within GFF1883at 76.600 kb on + strand, within GFF1883at 76.600 kb on + strand, within GFF1883at 76.601 kb on - strand, within GFF1883at 76.655 kb on - strand, within GFF1883at 76.679 kb on + strand, within GFF1883at 76.728 kb on - strandat 76.757 kb on + strandat 76.857 kb on - strand, within GFF1884

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI
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73,212 - GFF1880 0.65 -1.4
73,474 + GFF1880 0.83 -2.0
73,506 + GFF1880 0.85 -0.7
73,506 + GFF1880 0.85 -1.1
73,736 - -1.2
73,745 - -1.4
73,887 + -1.7
73,887 + -1.6
73,888 - -1.4
73,888 - -0.4
73,896 - -0.6
74,011 + -1.7
74,115 + -1.9
74,116 - -1.0
74,116 - -1.3
74,116 - -1.3
74,128 - -1.3
74,252 + GFF1881 0.11 -0.9
74,253 - GFF1881 0.11 -0.9
74,263 - GFF1881 0.11 -1.4
74,289 + GFF1881 0.13 -0.7
74,290 - GFF1881 0.13 -1.8
74,968 - GFF1881 0.50 -0.4
75,021 - GFF1881 0.53 -2.9
75,028 + GFF1881 0.53 -1.0
75,028 + GFF1881 0.53 -0.9
75,331 - GFF1881 0.70 +1.4
75,331 - GFF1881 0.70 -1.7
75,359 + GFF1881 0.71 -2.0
75,427 - GFF1881 0.75 -1.4
75,445 - GFF1881 0.76 -2.2
75,478 - GFF1881 0.78 -1.5
75,478 - GFF1881 0.78 +0.1
75,538 + GFF1881 0.81 -1.9
75,539 - GFF1881 0.81 -1.1
75,541 - GFF1881 0.81 -1.5
75,579 + GFF1881 0.83 -1.3
75,872 - -1.4
75,911 - -1.4
76,010 + GFF1882 0.39 -1.2
76,010 + GFF1882 0.39 -0.7
76,010 + GFF1882 0.39 -1.4
76,011 - GFF1882 0.39 -1.1
76,011 - GFF1882 0.39 -1.9
76,105 - GFF1882 0.70 -0.5
76,153 + GFF1882 0.86 -2.3
76,310 + GFF1883 0.21 -1.7
76,311 - GFF1883 0.22 -1.3
76,311 - GFF1883 0.22 -1.0
76,336 + GFF1883 0.26 -0.3
76,421 - GFF1883 0.41 -2.2
76,600 + GFF1883 0.74 -1.3
76,600 + GFF1883 0.74 -0.9
76,601 - GFF1883 0.74 -1.3
76,655 - GFF1883 0.84 -0.9
76,679 + GFF1883 0.88 -1.5
76,728 - -0.8
76,757 + -0.6
76,857 - GFF1884 0.19 -0.2

Or see this region's nucleotide sequence