Experiment: LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1335 and GFF1336 are separated by 209 nucleotides GFF1336 and GFF1337 overlap by 14 nucleotides GFF1337 and GFF1338 are separated by 143 nucleotides
GFF1335 - Nickel/cobalt efflux transporter RcnA, at 42,985 to 43,851
GFF1335
GFF1336 - FIG01047778: hypothetical protein, at 44,061 to 44,819
GFF1336
GFF1337 - FIG01045819: hypothetical protein, at 44,806 to 45,318
GFF1337
GFF1338 - Putative outer membrane protein, at 45,462 to 46,538
GFF1338
Position (kb) 44
45 Strain fitness (log2 ratio) -1
0
1 at 43.093 kb on - strand, within GFF1335 at 43.093 kb on - strand, within GFF1335 at 43.107 kb on + strand, within GFF1335 at 43.108 kb on - strand, within GFF1335 at 43.210 kb on + strand, within GFF1335 at 43.210 kb on + strand, within GFF1335 at 43.442 kb on + strand, within GFF1335 at 43.442 kb on + strand, within GFF1335 at 43.442 kb on + strand, within GFF1335 at 43.442 kb on + strand, within GFF1335 at 43.443 kb on - strand, within GFF1335 at 43.443 kb on - strand, within GFF1335 at 43.443 kb on - strand, within GFF1335 at 43.471 kb on + strand, within GFF1335 at 43.539 kb on + strand, within GFF1335 at 43.635 kb on + strand, within GFF1335 at 43.813 kb on + strand at 43.813 kb on + strand at 43.816 kb on - strand at 43.832 kb on - strand at 43.854 kb on - strand at 44.062 kb on + strand at 44.319 kb on - strand, within GFF1336 at 44.321 kb on - strand, within GFF1336 at 44.321 kb on - strand, within GFF1336 at 44.472 kb on + strand, within GFF1336 at 44.473 kb on - strand, within GFF1336 at 44.473 kb on - strand, within GFF1336 at 44.603 kb on - strand, within GFF1336 at 44.641 kb on - strand, within GFF1336 at 44.696 kb on - strand, within GFF1336 at 44.996 kb on + strand, within GFF1337 at 45.018 kb on + strand, within GFF1337 at 45.021 kb on + strand, within GFF1337 at 45.184 kb on - strand, within GFF1337 at 45.226 kb on - strand, within GFF1337
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI remove 43,093 - GFF1335 0.12 -0.5 43,093 - GFF1335 0.12 -0.2 43,107 + GFF1335 0.14 +0.8 43,108 - GFF1335 0.14 +0.1 43,210 + GFF1335 0.26 -0.1 43,210 + GFF1335 0.26 +0.6 43,442 + GFF1335 0.53 +0.2 43,442 + GFF1335 0.53 -0.0 43,442 + GFF1335 0.53 -0.4 43,442 + GFF1335 0.53 +1.0 43,443 - GFF1335 0.53 -0.0 43,443 - GFF1335 0.53 -0.8 43,443 - GFF1335 0.53 +0.1 43,471 + GFF1335 0.56 +1.5 43,539 + GFF1335 0.64 +0.2 43,635 + GFF1335 0.75 -0.3 43,813 + +0.2 43,813 + -0.0 43,816 - -0.4 43,832 - -0.0 43,854 - -0.1 44,062 + -0.3 44,319 - GFF1336 0.34 -0.0 44,321 - GFF1336 0.34 -0.0 44,321 - GFF1336 0.34 +0.8 44,472 + GFF1336 0.54 +0.5 44,473 - GFF1336 0.54 +0.7 44,473 - GFF1336 0.54 -0.0 44,603 - GFF1336 0.71 +0.4 44,641 - GFF1336 0.76 +0.1 44,696 - GFF1336 0.84 -0.1 44,996 + GFF1337 0.37 -0.2 45,018 + GFF1337 0.41 -0.1 45,021 + GFF1337 0.42 -0.3 45,184 - GFF1337 0.74 -0.3 45,226 - GFF1337 0.82 -0.4
Or see this region's nucleotide sequence