Experiment: LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF1011 and GFF1012 are separated by 101 nucleotides GFF1012 and GFF1013 are separated by 14 nucleotides GFF1013 and GFF1014 are separated by 25 nucleotides GFF1014 and GFF1015 overlap by 23 nucleotides
GFF1011 - Transcriptional regulatory protein levR, at 195,419 to 198,184
GFF1011
GFF1012 - PTS system, mannose-specific IIA component (EC 2.7.1.69), at 198,286 to 198,708
GFF1012
GFF1013 - PTS system, mannose-specific IIB component (EC 2.7.1.69), at 198,723 to 199,184
GFF1013
GFF1014 - PTS system, mannose-specific IIC component (EC 2.7.1.69), at 199,210 to 199,989
GFF1014
GFF1015 - PTS system, mannose-specific IID component (EC 2.7.1.69), at 199,967 to 200,815
GFF1015
Position (kb) 198
199
200 Strain fitness (log2 ratio) -1
0
1 at 197.754 kb on - strand, within GFF1011 at 197.754 kb on - strand, within GFF1011 at 197.963 kb on + strand at 197.964 kb on - strand at 197.964 kb on - strand at 197.964 kb on - strand at 197.971 kb on + strand at 197.971 kb on + strand at 197.971 kb on + strand at 197.975 kb on - strand at 197.975 kb on - strand at 198.092 kb on + strand at 198.093 kb on - strand at 198.093 kb on - strand at 198.137 kb on - strand at 198.359 kb on + strand, within GFF1012 at 198.438 kb on - strand, within GFF1012 at 198.615 kb on + strand, within GFF1012 at 198.806 kb on + strand, within GFF1013 at 198.833 kb on + strand, within GFF1013 at 199.206 kb on + strand at 199.207 kb on - strand at 199.210 kb on - strand at 199.210 kb on - strand at 199.531 kb on - strand, within GFF1014
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.0001875 MOI remove 197,754 - GFF1011 0.84 +0.6 197,754 - GFF1011 0.84 -0.2 197,963 + -0.1 197,964 - +1.4 197,964 - -0.5 197,964 - -0.0 197,971 + +0.1 197,971 + +0.5 197,971 + +0.8 197,975 - +0.6 197,975 - -0.2 198,092 + -0.1 198,093 - +0.0 198,093 - -0.7 198,137 - -0.3 198,359 + GFF1012 0.17 +0.3 198,438 - GFF1012 0.36 +0.7 198,615 + GFF1012 0.78 -0.0 198,806 + GFF1013 0.18 +0.8 198,833 + GFF1013 0.24 +0.6 199,206 + +0.1 199,207 - -0.1 199,210 - -0.6 199,210 - +1.0 199,531 - GFF1014 0.41 -0.2
Or see this region's nucleotide sequence