Experiment: capsaicin 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvB and ruvA are separated by 8 nucleotides ruvA and yobI are separated by 138 nucleotides yobI and yebB are separated by 70 nucleotides yebB and ruvC are separated by 1 nucleotides
b1860: ruvB - Holliday junction DNA helicase B (NCBI), at 1,942,370 to 1,943,380
ruvB
b1861: ruvA - Holliday junction DNA helicase motor protein (NCBI), at 1,943,389 to 1,944,000
ruvA
b4677: yobI - expressed protein (RefSeq), at 1,944,139 to 1,944,204
yobI
b1862: yebB - orf, hypothetical protein (VIMSS), at 1,944,275 to 1,944,877
yebB
b1863: ruvC - Holliday junction resolvase (NCBI), at 1,944,879 to 1,945,400
ruvC
Position (kb)
1943
1944
1945 Strain fitness (log2 ratio)
-2
-1
0
1 at 1942.423 kb on + strand at 1942.445 kb on - strand at 1942.446 kb on - strand at 1942.806 kb on + strand, within ruvB at 1942.985 kb on + strand, within ruvB at 1943.892 kb on + strand, within ruvA at 1943.919 kb on - strand, within ruvA at 1943.934 kb on - strand, within ruvA at 1943.990 kb on - strand at 1943.996 kb on + strand at 1943.996 kb on + strand at 1944.021 kb on + strand at 1944.102 kb on + strand at 1944.102 kb on + strand at 1944.137 kb on + strand at 1944.333 kb on - strand at 1944.333 kb on - strand at 1944.511 kb on - strand, within yebB at 1944.560 kb on - strand, within yebB at 1944.582 kb on + strand, within yebB at 1944.622 kb on + strand, within yebB at 1944.625 kb on - strand, within yebB at 1944.625 kb on - strand, within yebB at 1944.625 kb on - strand, within yebB at 1944.669 kb on - strand, within yebB at 1944.674 kb on - strand, within yebB at 1944.723 kb on - strand, within yebB at 1944.818 kb on + strand at 1944.818 kb on + strand at 1944.826 kb on - strand at 1944.826 kb on - strand at 1944.829 kb on + strand at 1944.846 kb on - strand at 1944.885 kb on - strand at 1944.885 kb on - strand at 1944.895 kb on - strand at 1944.908 kb on + strand at 1944.916 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction capsaicin 2 mM remove 1,942,423 + -1.6 1,942,445 - -0.3 1,942,446 - -0.4 1,942,806 + ruvB b1860 0.43 +1.1 1,942,985 + ruvB b1860 0.61 -0.3 1,943,892 + ruvA b1861 0.82 -0.7 1,943,919 - ruvA b1861 0.87 -1.5 1,943,934 - ruvA b1861 0.89 +0.5 1,943,990 - -1.7 1,943,996 + -0.7 1,943,996 + -0.3 1,944,021 + +0.2 1,944,102 + +1.2 1,944,102 + -1.3 1,944,137 + -0.5 1,944,333 - +0.1 1,944,333 - +0.5 1,944,511 - yebB b1862 0.39 -0.3 1,944,560 - yebB b1862 0.47 -1.4 1,944,582 + yebB b1862 0.51 +0.0 1,944,622 + yebB b1862 0.58 +0.5 1,944,625 - yebB b1862 0.58 +0.0 1,944,625 - yebB b1862 0.58 -0.3 1,944,625 - yebB b1862 0.58 +1.0 1,944,669 - yebB b1862 0.65 +0.9 1,944,674 - yebB b1862 0.66 +1.0 1,944,723 - yebB b1862 0.74 +0.2 1,944,818 + +0.3 1,944,818 + +1.4 1,944,826 - +0.6 1,944,826 - +0.4 1,944,829 + +0.2 1,944,846 - +0.3 1,944,885 - -0.1 1,944,885 - -2.0 1,944,895 - -0.1 1,944,908 + +1.2 1,944,916 - -0.3
Or see this region's nucleotide sequence