Experiment: capsaicin 2 mM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt mraZ and mraW are separated by 1 nucleotides mraW and ftsL overlap by 4 nucleotides ftsL and ftsI are separated by 15 nucleotides
b0081: mraZ - hypothetical protein (NCBI), at 89,634 to 90,092
mraZ
b0082: mraW - S-adenosyl-methyltransferase (NCBI), at 90,094 to 91,035
mraW
b0083: ftsL - membrane bound cell division protein at septum containing leucine zipper motif (NCBI), at 91,032 to 91,397
ftsL
b0084: ftsI - transpeptidase involved in septal peptidoglycan synthesis (penicillin-binding protein 3) (NCBI), at 91,413 to 93,179
ftsI
Position (kb) 90
91
92 Strain fitness (log2 ratio) -1
0
1
2 at 89.122 kb on + strand at 89.132 kb on + strand at 89.225 kb on + strand at 89.294 kb on - strand at 89.307 kb on + strand at 89.307 kb on + strand at 89.312 kb on + strand at 89.312 kb on + strand at 89.312 kb on + strand at 89.313 kb on - strand at 89.315 kb on - strand at 89.325 kb on + strand at 89.325 kb on - strand at 89.330 kb on - strand at 89.391 kb on + strand at 89.393 kb on + strand at 89.401 kb on - strand at 89.411 kb on + strand at 89.411 kb on + strand at 89.416 kb on - strand at 89.508 kb on + strand at 89.508 kb on + strand at 89.542 kb on - strand at 89.542 kb on - strand at 89.550 kb on + strand at 89.558 kb on - strand at 89.649 kb on + strand at 89.649 kb on + strand at 89.658 kb on + strand at 89.680 kb on + strand, within mraZ at 89.685 kb on + strand, within mraZ at 89.917 kb on + strand, within mraZ at 89.917 kb on + strand, within mraZ at 89.981 kb on + strand, within mraZ at 90.039 kb on + strand, within mraZ at 90.039 kb on + strand, within mraZ at 90.042 kb on + strand, within mraZ at 90.042 kb on + strand, within mraZ at 90.230 kb on + strand, within mraW at 90.473 kb on + strand, within mraW at 90.518 kb on + strand, within mraW at 90.544 kb on + strand, within mraW at 90.681 kb on + strand, within mraW at 90.701 kb on + strand, within mraW at 90.783 kb on + strand, within mraW at 90.887 kb on + strand, within mraW at 90.896 kb on + strand, within mraW at 90.899 kb on + strand, within mraW at 90.979 kb on + strand at 91.008 kb on + strand at 91.020 kb on + strand at 91.020 kb on + strand at 91.020 kb on + strand at 91.028 kb on + strand at 91.028 kb on + strand at 91.357 kb on + strand at 91.362 kb on + strand at 91.378 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction capsaicin 2 mM remove 89,122 + +0.3 89,132 + +0.0 89,225 + +0.4 89,294 - -0.0 89,307 + -1.7 89,307 + +0.7 89,312 + -0.0 89,312 + +0.4 89,312 + +0.4 89,313 - +0.0 89,315 - +0.8 89,325 + -0.2 89,325 - -0.8 89,330 - +0.0 89,391 + +0.2 89,393 + +0.1 89,401 - +0.4 89,411 + -0.2 89,411 + -0.2 89,416 - +0.2 89,508 + +0.4 89,508 + +0.1 89,542 - +0.2 89,542 - +0.3 89,550 + -0.5 89,558 - +0.4 89,649 + -0.1 89,649 + +0.1 89,658 + +2.3 89,680 + mraZ b0081 0.10 +0.2 89,685 + mraZ b0081 0.11 -1.2 89,917 + mraZ b0081 0.62 +0.0 89,917 + mraZ b0081 0.62 -0.1 89,981 + mraZ b0081 0.76 +0.0 90,039 + mraZ b0081 0.88 +0.6 90,039 + mraZ b0081 0.88 -0.0 90,042 + mraZ b0081 0.89 +0.6 90,042 + mraZ b0081 0.89 +1.3 90,230 + mraW b0082 0.14 -0.4 90,473 + mraW b0082 0.40 -0.3 90,518 + mraW b0082 0.45 -0.5 90,544 + mraW b0082 0.48 -0.6 90,681 + mraW b0082 0.62 -0.8 90,701 + mraW b0082 0.64 -0.2 90,783 + mraW b0082 0.73 -0.3 90,887 + mraW b0082 0.84 -0.2 90,896 + mraW b0082 0.85 -1.3 90,899 + mraW b0082 0.85 -0.5 90,979 + +0.3 91,008 + -0.4 91,020 + -0.5 91,020 + -0.0 91,020 + -0.1 91,028 + -0.9 91,028 + +1.1 91,357 + +2.2 91,362 + +0.4 91,378 + -0.3
Or see this region's nucleotide sequence