Strain Fitness in Escherichia coli BW25113 around b0028

Experiment: capsaicin 2 mM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntileS and lspA overlap by 1 nucleotideslspA and fkpB are separated by 124 nucleotidesfkpB and ispH are separated by 1 nucleotides b0026: ileS - isoleucyl-tRNA synthetase (NCBI), at 22,391 to 25,207 ileS b0027: lspA - signal peptidase II (NCBI), at 25,207 to 25,701 lspA b0028: fkpB - FKBP-type peptidyl-prolyl cis-trans isomerase (rotamase) (NCBI), at 25,826 to 26,275 fkpB b0029: ispH - 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (NCBI), at 26,277 to 27,227 ispH Position (kb) 25 26 27Strain fitness (log2 ratio) -2 -1 0 1at 25.846 kb on - strandat 25.846 kb on - strandat 25.918 kb on + strand, within fkpBat 26.033 kb on + strand, within fkpBat 26.142 kb on + strand, within fkpBat 26.145 kb on - strand, within fkpBat 26.145 kb on - strand, within fkpBat 26.147 kb on + strand, within fkpBat 26.190 kb on - strand, within fkpBat 26.235 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction capsaicin 2 mM
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25,846 - -0.7
25,846 - -2.6
25,918 + fkpB b0028 0.20 +0.1
26,033 + fkpB b0028 0.46 +0.3
26,142 + fkpB b0028 0.70 -0.6
26,145 - fkpB b0028 0.71 -0.0
26,145 - fkpB b0028 0.71 -0.1
26,147 + fkpB b0028 0.71 +0.1
26,190 - fkpB b0028 0.81 -1.8
26,235 + -0.0

Or see this region's nucleotide sequence