Experiment: LB_plus_SM_buffer with Ffm_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4756 and GFF4757 overlap by 11 nucleotides GFF4757 and GFF4758 overlap by 1 nucleotides GFF4758 and GFF4759 overlap by 1 nucleotides GFF4759 and GFF4760 overlap by 23 nucleotides
GFF4756 - Type III secretion inner membrane protein (YscR,SpaR,HrcR,EscR,homologous to flagellar export components), at 63,454 to 64,128
GFF4756
GFF4757 - Type III secretion inner membrane protein (YscQ,homologous to flagellar export components); Surface presentation of antigens protein SpaO, at 64,118 to 65,029
GFF4757
GFF4758 - Type III secretion host injection and negative regulator protein (YopD), at 65,029 to 66,039
GFF4758
GFF4759 - Surface presentation of antigens protein SpaM, at 66,039 to 66,482
GFF4759
GFF4760 - Type III secretion cytoplasmic ATP synthase (EC 3.6.3.14, YscN,SpaL,MxiB,HrcN,EscN), at 66,460 to 67,755
GFF4760
Position (kb) 65
66
67 Strain fitness (log2 ratio) -1
0
1 at 64.509 kb on - strand, within GFF4757 at 64.721 kb on + strand, within GFF4757 at 65.164 kb on - strand, within GFF4758 at 65.219 kb on + strand, within GFF4758 at 65.247 kb on - strand, within GFF4758 at 65.259 kb on + strand, within GFF4758 at 65.260 kb on - strand, within GFF4758 at 65.415 kb on + strand, within GFF4758 at 65.425 kb on - strand, within GFF4758 at 65.526 kb on + strand, within GFF4758 at 65.551 kb on - strand, within GFF4758 at 65.585 kb on - strand, within GFF4758 at 65.639 kb on - strand, within GFF4758 at 66.092 kb on - strand, within GFF4759 at 66.268 kb on - strand, within GFF4759 at 66.456 kb on + strand at 66.457 kb on - strand at 66.716 kb on - strand, within GFF4760 at 66.777 kb on - strand, within GFF4760 at 66.855 kb on - strand, within GFF4760
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.001875 MOI remove 64,509 - GFF4757 0.43 +0.1 64,721 + GFF4757 0.66 -0.1 65,164 - GFF4758 0.13 -0.3 65,219 + GFF4758 0.19 -0.3 65,247 - GFF4758 0.22 -0.7 65,259 + GFF4758 0.23 -0.4 65,260 - GFF4758 0.23 +0.2 65,415 + GFF4758 0.38 -0.4 65,425 - GFF4758 0.39 -0.0 65,526 + GFF4758 0.49 +0.5 65,551 - GFF4758 0.52 -0.1 65,585 - GFF4758 0.55 -0.9 65,639 - GFF4758 0.60 -0.2 66,092 - GFF4759 0.12 -0.3 66,268 - GFF4759 0.52 +0.1 66,456 + +0.3 66,457 - +0.6 66,716 - GFF4760 0.20 +0.9 66,777 - GFF4760 0.24 -0.1 66,855 - GFF4760 0.30 +0.6
Or see this region's nucleotide sequence