Experiment: LB_plus_SM_buffer with Ffm_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4697 and GFF4698 are separated by 11 nucleotides GFF4698 and GFF4699 are separated by 11 nucleotides GFF4699 and GFF4700 are separated by 75 nucleotides GFF4700 and GFF4701 are separated by 197 nucleotides
GFF4697 - PTS system, glucitol/sorbitol-specific IIB component and second of two IIC components (EC 2.7.1.69), at 8,648 to 9,619
GFF4697
GFF4698 - PTS system, glucitol/sorbitol-specific IIA component (EC 2.7.1.69), at 9,631 to 9,993
GFF4698
GFF4699 - Sorbitol-6-phosphate 2-dehydrogenase (EC 1.1.1.140), at 10,005 to 10,784
GFF4699
GFF4700 - Glucitol operon activator protein, at 10,860 to 11,219
GFF4700
GFF4701 - Glucitol operon repressor, at 11,417 to 12,190
GFF4701
Position (kb)
10
11 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 9.142 kb on - strand, within GFF4697 at 9.233 kb on - strand, within GFF4697 at 9.233 kb on - strand, within GFF4697 at 9.579 kb on + strand at 9.580 kb on - strand at 9.608 kb on - strand at 9.618 kb on - strand at 9.709 kb on - strand, within GFF4698 at 9.709 kb on - strand, within GFF4698 at 9.880 kb on - strand, within GFF4698 at 9.929 kb on + strand, within GFF4698 at 10.104 kb on - strand, within GFF4699 at 10.264 kb on - strand, within GFF4699 at 10.428 kb on - strand, within GFF4699 at 10.540 kb on + strand, within GFF4699 at 10.645 kb on - strand, within GFF4699 at 10.647 kb on + strand, within GFF4699 at 10.647 kb on + strand, within GFF4699 at 10.648 kb on - strand, within GFF4699 at 10.648 kb on - strand, within GFF4699 at 10.714 kb on - strand at 10.859 kb on + strand at 10.860 kb on - strand at 10.961 kb on + strand, within GFF4700 at 11.006 kb on + strand, within GFF4700 at 11.323 kb on + strand at 11.323 kb on + strand at 11.323 kb on + strand at 11.323 kb on + strand at 11.380 kb on - strand at 11.393 kb on - strand at 11.396 kb on + strand at 11.498 kb on + strand, within GFF4701 at 11.574 kb on - strand, within GFF4701 at 11.678 kb on + strand, within GFF4701 at 11.678 kb on + strand, within GFF4701 at 11.679 kb on - strand, within GFF4701 at 11.727 kb on + strand, within GFF4701
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.001875 MOI remove 9,142 - GFF4697 0.51 -0.0 9,233 - GFF4697 0.60 +0.5 9,233 - GFF4697 0.60 -0.2 9,579 + -0.2 9,580 - -0.1 9,608 - +0.1 9,618 - +0.1 9,709 - GFF4698 0.21 +0.5 9,709 - GFF4698 0.21 +0.4 9,880 - GFF4698 0.69 +0.2 9,929 + GFF4698 0.82 -0.4 10,104 - GFF4699 0.13 -1.1 10,264 - GFF4699 0.33 +0.7 10,428 - GFF4699 0.54 +1.1 10,540 + GFF4699 0.69 -0.1 10,645 - GFF4699 0.82 -0.0 10,647 + GFF4699 0.82 +0.1 10,647 + GFF4699 0.82 +0.7 10,648 - GFF4699 0.82 +0.7 10,648 - GFF4699 0.82 +0.3 10,714 - -0.9 10,859 + +0.4 10,860 - +0.3 10,961 + GFF4700 0.28 +0.4 11,006 + GFF4700 0.41 -0.6 11,323 + -0.7 11,323 + +0.3 11,323 + +0.2 11,323 + -0.0 11,380 - -0.7 11,393 - +0.0 11,396 + -0.2 11,498 + GFF4701 0.10 -0.2 11,574 - GFF4701 0.20 -0.7 11,678 + GFF4701 0.34 +0.4 11,678 + GFF4701 0.34 -0.4 11,679 - GFF4701 0.34 -0.4 11,727 + GFF4701 0.40 -3.1
Or see this region's nucleotide sequence