Strain Fitness in Salmonella enterica subsp. enterica serovar Typhimurium str. MS1868 around GFF4320

Experiment: LB_plus_SM_buffer with Ffm_phage 0.001875 MOI

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntGFF4318 and GFF4319 are separated by 88 nucleotidesGFF4319 and GFF4320 are separated by 20 nucleotidesGFF4320 and GFF4321 are separated by 78 nucleotides GFF4318 - Diaminohydroxyphosphoribosylaminopyrimidine deaminase (EC 3.5.4.26) / 5-amino-6-(5-phosphoribosylamino)uracil reductase (EC 1.1.1.193), at 100,056 to 101,159 GFF4318 GFF4319 - 6,7-dimethyl-8-ribityllumazine synthase (EC 2.5.1.78), at 101,248 to 101,718 GFF4319 GFF4320 - Transcription termination protein NusB, at 101,739 to 102,158 GFF4320 GFF4321 - Thiamine-monophosphate kinase (EC 2.7.4.16), at 102,237 to 103,214 GFF4321 Position (kb) 101 102 103Strain fitness (log2 ratio) -1 0 1at 101.144 kb on + strandat 101.144 kb on + strandat 101.144 kb on + strandat 102.434 kb on + strand, within GFF4321

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Per-strain Table

Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.001875 MOI
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101,144 + -0.0
101,144 + -0.2
101,144 + +0.4
102,434 + GFF4321 0.20 -0.5

Or see this region's nucleotide sequence