Experiment: LB_plus_SM_buffer with Ffm_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF4282 and GFF4283 are separated by 14 nucleotides GFF4283 and GFF4284 are separated by 33 nucleotides GFF4284 and GFF4285 overlap by 44 nucleotides GFF4285 and GFF4286 are separated by 184 nucleotides GFF4286 and GFF4287 are separated by 26 nucleotides
GFF4282 - Protein YaiC, at 68,341 to 69,453
GFF4282
GFF4283 - Pyrroline-5-carboxylate reductase (EC 1.5.1.2), at 69,468 to 70,277
GFF4283
GFF4284 - hypothetical protein, at 70,311 to 70,460
GFF4284
GFF4285 - Protein YaiI, at 70,417 to 70,872
GFF4285
GFF4286 - Shikimate kinase III (EC 2.7.1.71), at 71,057 to 71,602
GFF4286
GFF4287 - Protein YaiA, at 71,629 to 71,820
GFF4287
Position (kb) 70
71 Strain fitness (log2 ratio) -1
0
1
2 at 69.429 kb on + strand at 69.519 kb on + strand at 69.520 kb on - strand at 69.597 kb on - strand, within GFF4283 at 69.675 kb on - strand, within GFF4283 at 69.676 kb on + strand, within GFF4283 at 69.737 kb on + strand, within GFF4283 at 69.738 kb on - strand, within GFF4283 at 69.738 kb on - strand, within GFF4283 at 69.745 kb on + strand, within GFF4283 at 69.745 kb on + strand, within GFF4283 at 70.052 kb on + strand, within GFF4283 at 70.174 kb on + strand, within GFF4283 at 70.175 kb on - strand, within GFF4283 at 70.175 kb on - strand, within GFF4283 at 70.197 kb on + strand at 70.241 kb on + strand at 70.257 kb on - strand at 70.397 kb on + strand, within GFF4284 at 70.399 kb on + strand, within GFF4284 at 70.405 kb on - strand, within GFF4284 at 70.417 kb on - strand at 70.417 kb on - strand at 70.459 kb on - strand at 70.707 kb on + strand, within GFF4285 at 70.775 kb on + strand, within GFF4285 at 70.929 kb on + strand at 71.029 kb on - strand at 71.072 kb on + strand at 71.072 kb on + strand at 71.297 kb on - strand, within GFF4286 at 71.299 kb on + strand, within GFF4286 at 71.312 kb on + strand, within GFF4286 at 71.315 kb on - strand, within GFF4286 at 71.315 kb on - strand, within GFF4286 at 71.325 kb on - strand, within GFF4286 at 71.326 kb on + strand, within GFF4286 at 71.326 kb on + strand, within GFF4286 at 71.327 kb on - strand, within GFF4286 at 71.327 kb on - strand, within GFF4286 at 71.327 kb on - strand, within GFF4286 at 71.327 kb on - strand, within GFF4286 at 71.327 kb on - strand, within GFF4286 at 71.351 kb on + strand, within GFF4286 at 71.352 kb on - strand, within GFF4286 at 71.502 kb on - strand, within GFF4286 at 71.596 kb on + strand at 71.601 kb on - strand at 71.662 kb on + strand, within GFF4287
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.001875 MOI remove 69,429 + +0.3 69,519 + -0.5 69,520 - -1.1 69,597 - GFF4283 0.16 -0.1 69,675 - GFF4283 0.26 +0.6 69,676 + GFF4283 0.26 +0.1 69,737 + GFF4283 0.33 +0.2 69,738 - GFF4283 0.33 -0.4 69,738 - GFF4283 0.33 +0.0 69,745 + GFF4283 0.34 +0.0 69,745 + GFF4283 0.34 +0.3 70,052 + GFF4283 0.72 +0.1 70,174 + GFF4283 0.87 +0.4 70,175 - GFF4283 0.87 +0.1 70,175 - GFF4283 0.87 +1.0 70,197 + -0.1 70,241 + -0.2 70,257 - +0.1 70,397 + GFF4284 0.57 -0.3 70,399 + GFF4284 0.59 +0.3 70,405 - GFF4284 0.63 +0.4 70,417 - +1.5 70,417 - +0.1 70,459 - +0.5 70,707 + GFF4285 0.64 -0.1 70,775 + GFF4285 0.79 -0.8 70,929 + -0.2 71,029 - +0.3 71,072 + +0.1 71,072 + +0.5 71,297 - GFF4286 0.44 +0.4 71,299 + GFF4286 0.44 +1.0 71,312 + GFF4286 0.47 +0.2 71,315 - GFF4286 0.47 +1.6 71,315 - GFF4286 0.47 -0.1 71,325 - GFF4286 0.49 -0.1 71,326 + GFF4286 0.49 +0.5 71,326 + GFF4286 0.49 +1.9 71,327 - GFF4286 0.49 +0.2 71,327 - GFF4286 0.49 -0.1 71,327 - GFF4286 0.49 +0.4 71,327 - GFF4286 0.49 -0.4 71,327 - GFF4286 0.49 +0.1 71,351 + GFF4286 0.54 +0.3 71,352 - GFF4286 0.54 +0.4 71,502 - GFF4286 0.82 +0.3 71,596 + +0.2 71,601 - -0.3 71,662 + GFF4287 0.17 -0.6
Or see this region's nucleotide sequence