Experiment: LB_plus_SM_buffer with Ffm_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3690 and GFF3691 overlap by 4 nucleotides GFF3691 and GFF3692 are separated by 98 nucleotides GFF3692 and GFF3693 are separated by 72 nucleotides GFF3693 and GFF3694 are separated by 13 nucleotides
GFF3690 - Leucine-responsive regulatory protein, regulator for leucine (or lrp) regulon and high-affinity branched-chain amino acid transport system, at 44,680 to 45,141
GFF3690
GFF3691 - FIG01047584: hypothetical protein, at 45,138 to 45,359
GFF3691
GFF3692 - Threonine dehydratase (EC 4.3.1.19), at 45,458 to 46,672
GFF3692
GFF3693 - FIG01045169: hypothetical protein, at 46,745 to 46,903
GFF3693
GFF3694 - Putative ion:amino acid symporter, at 46,917 to 48,353
GFF3694
Position (kb)
45
46
47 Strain fitness (log2 ratio)
-1
0
1 at 44.846 kb on + strand, within GFF3690 at 44.846 kb on + strand, within GFF3690 at 44.847 kb on - strand, within GFF3690 at 44.847 kb on - strand, within GFF3690 at 44.847 kb on - strand, within GFF3690 at 45.880 kb on - strand, within GFF3692 at 45.975 kb on - strand, within GFF3692 at 46.088 kb on + strand, within GFF3692 at 46.441 kb on + strand, within GFF3692 at 47.670 kb on + strand, within GFF3694
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.001875 MOI remove 44,846 + GFF3690 0.36 -0.7 44,846 + GFF3690 0.36 -0.2 44,847 - GFF3690 0.36 -0.5 44,847 - GFF3690 0.36 -0.3 44,847 - GFF3690 0.36 -0.6 45,880 - GFF3692 0.35 +0.5 45,975 - GFF3692 0.43 -1.0 46,088 + GFF3692 0.52 +0.1 46,441 + GFF3692 0.81 -0.3 47,670 + GFF3694 0.52 -0.1
Or see this region's nucleotide sequence