Experiment: LB_plus_SM_buffer with Ffm_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF3561 and GFF3562 overlap by 4 nucleotides GFF3562 and GFF3563 are separated by 12 nucleotides GFF3563 and GFF3564 are separated by 154 nucleotides
GFF3561 - Iron-sulfur cluster assembly protein SufD, at 316,970 to 318,241
GFF3561
GFF3562 - Cysteine desulfurase (EC 2.8.1.7), SufS subfamily, at 318,238 to 319,458
GFF3562
GFF3563 - Sulfur acceptor protein SufE for iron-sulfur cluster assembly, at 319,471 to 319,887
GFF3563
GFF3564 - L,D-transpeptidase YnhG, at 320,042 to 321,043
GFF3564
Position (kb) 318
319
320 Strain fitness (log2 ratio) -1
0
1 at 317.266 kb on + strand, within GFF3561 at 317.384 kb on + strand, within GFF3561 at 317.384 kb on + strand, within GFF3561 at 317.384 kb on + strand, within GFF3561 at 317.384 kb on + strand, within GFF3561 at 317.385 kb on - strand, within GFF3561 at 317.388 kb on + strand, within GFF3561 at 317.388 kb on + strand, within GFF3561 at 317.388 kb on + strand, within GFF3561 at 317.389 kb on - strand, within GFF3561 at 317.431 kb on - strand, within GFF3561 at 317.437 kb on + strand, within GFF3561 at 317.546 kb on - strand, within GFF3561 at 317.546 kb on - strand, within GFF3561 at 317.727 kb on + strand, within GFF3561 at 317.728 kb on - strand, within GFF3561 at 317.782 kb on + strand, within GFF3561 at 317.783 kb on - strand, within GFF3561 at 317.839 kb on + strand, within GFF3561 at 317.839 kb on + strand, within GFF3561 at 317.854 kb on + strand, within GFF3561 at 317.854 kb on + strand, within GFF3561 at 318.024 kb on - strand, within GFF3561 at 318.068 kb on - strand, within GFF3561 at 318.314 kb on - strand at 318.346 kb on - strand at 318.376 kb on + strand, within GFF3562 at 318.474 kb on + strand, within GFF3562 at 318.585 kb on + strand, within GFF3562 at 318.687 kb on + strand, within GFF3562 at 318.688 kb on - strand, within GFF3562 at 318.707 kb on + strand, within GFF3562 at 319.137 kb on + strand, within GFF3562 at 319.213 kb on + strand, within GFF3562 at 319.214 kb on - strand, within GFF3562 at 319.293 kb on + strand, within GFF3562 at 319.391 kb on - strand at 319.391 kb on - strand at 319.391 kb on - strand at 319.507 kb on - strand at 319.548 kb on + strand, within GFF3563 at 319.548 kb on + strand, within GFF3563 at 319.548 kb on + strand, within GFF3563 at 319.725 kb on - strand, within GFF3563 at 319.735 kb on + strand, within GFF3563 at 319.736 kb on - strand, within GFF3563 at 320.166 kb on - strand, within GFF3564 at 320.167 kb on + strand, within GFF3564 at 320.200 kb on + strand, within GFF3564 at 320.269 kb on + strand, within GFF3564 at 320.287 kb on + strand, within GFF3564 at 320.308 kb on - strand, within GFF3564 at 320.335 kb on + strand, within GFF3564 at 320.364 kb on - strand, within GFF3564 at 320.366 kb on + strand, within GFF3564 at 320.392 kb on - strand, within GFF3564 at 320.399 kb on + strand, within GFF3564
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.001875 MOI remove 317,266 + GFF3561 0.23 +0.0 317,384 + GFF3561 0.33 +0.4 317,384 + GFF3561 0.33 +0.5 317,384 + GFF3561 0.33 +0.3 317,384 + GFF3561 0.33 +0.4 317,385 - GFF3561 0.33 -0.2 317,388 + GFF3561 0.33 -0.2 317,388 + GFF3561 0.33 -0.4 317,388 + GFF3561 0.33 +0.5 317,389 - GFF3561 0.33 -0.5 317,431 - GFF3561 0.36 +0.6 317,437 + GFF3561 0.37 +0.3 317,546 - GFF3561 0.45 -0.0 317,546 - GFF3561 0.45 -0.2 317,727 + GFF3561 0.60 +1.2 317,728 - GFF3561 0.60 +0.0 317,782 + GFF3561 0.64 +0.2 317,783 - GFF3561 0.64 -0.1 317,839 + GFF3561 0.68 -0.4 317,839 + GFF3561 0.68 +0.1 317,854 + GFF3561 0.69 -0.2 317,854 + GFF3561 0.69 +0.3 318,024 - GFF3561 0.83 +0.2 318,068 - GFF3561 0.86 -0.0 318,314 - +0.3 318,346 - +0.8 318,376 + GFF3562 0.11 +0.5 318,474 + GFF3562 0.19 -0.0 318,585 + GFF3562 0.28 -0.3 318,687 + GFF3562 0.37 +0.5 318,688 - GFF3562 0.37 +0.4 318,707 + GFF3562 0.38 +1.3 319,137 + GFF3562 0.74 +0.1 319,213 + GFF3562 0.80 -0.2 319,214 - GFF3562 0.80 -0.2 319,293 + GFF3562 0.86 +0.4 319,391 - -0.6 319,391 - +0.9 319,391 - -0.3 319,507 - -0.2 319,548 + GFF3563 0.18 -0.6 319,548 + GFF3563 0.18 +0.4 319,548 + GFF3563 0.18 +0.0 319,725 - GFF3563 0.61 +0.8 319,735 + GFF3563 0.63 -0.9 319,736 - GFF3563 0.64 -0.6 320,166 - GFF3564 0.12 +0.4 320,167 + GFF3564 0.12 +0.3 320,200 + GFF3564 0.16 -0.3 320,269 + GFF3564 0.23 +0.3 320,287 + GFF3564 0.24 -0.2 320,308 - GFF3564 0.27 -0.1 320,335 + GFF3564 0.29 -0.8 320,364 - GFF3564 0.32 -1.7 320,366 + GFF3564 0.32 +0.2 320,392 - GFF3564 0.35 +0.4 320,399 + GFF3564 0.36 -0.2
Or see this region's nucleotide sequence