Experiment: LB_plus_SM_buffer with Ffm_phage 0.001875 MOI
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt GFF2896 and GFF2897 are separated by 163 nucleotides GFF2897 and GFF2898 are separated by 2 nucleotides GFF2898 and GFF2899 overlap by 4 nucleotides GFF2899 and GFF2900 are separated by 283 nucleotides
GFF2896 - Ketol-acid reductoisomerase (EC 1.1.1.86), at 22,338 to 23,813
GFF2896
GFF2897 - HTH-type transcriptional regulator IlvY, at 23,977 to 24,864
GFF2897
GFF2898 - FIG074102: hypothetical protein, at 24,867 to 25,208
GFF2898
GFF2899 - Phage-related protein, at 25,205 to 25,522
GFF2899
GFF2900 - Threonine dehydratase biosynthetic (EC 4.3.1.19), at 25,806 to 27,350
GFF2900
Position (kb)
23
24
25 Strain fitness (log2 ratio)
-1
0
1 at 23.321 kb on - strand, within GFF2896 at 23.382 kb on - strand, within GFF2896 at 23.508 kb on + strand, within GFF2896 at 23.509 kb on - strand, within GFF2896 at 23.620 kb on + strand, within GFF2896 at 23.627 kb on + strand, within GFF2896 at 23.760 kb on - strand at 23.795 kb on + strand at 23.796 kb on - strand at 23.799 kb on - strand at 23.803 kb on + strand at 23.804 kb on - strand at 23.822 kb on - strand at 23.822 kb on - strand at 23.848 kb on - strand at 23.869 kb on + strand at 23.893 kb on - strand at 23.893 kb on - strand at 23.947 kb on + strand at 24.258 kb on + strand, within GFF2897 at 24.283 kb on + strand, within GFF2897 at 24.635 kb on - strand, within GFF2897 at 24.876 kb on + strand at 24.915 kb on - strand, within GFF2898 at 24.987 kb on - strand, within GFF2898 at 24.987 kb on - strand, within GFF2898 at 24.999 kb on - strand, within GFF2898 at 25.056 kb on + strand, within GFF2898 at 25.057 kb on - strand, within GFF2898 at 25.057 kb on - strand, within GFF2898 at 25.130 kb on + strand, within GFF2898 at 25.240 kb on + strand, within GFF2899 at 25.304 kb on - strand, within GFF2899 at 25.341 kb on + strand, within GFF2899 at 25.341 kb on + strand, within GFF2899 at 25.342 kb on - strand, within GFF2899 at 25.342 kb on - strand, within GFF2899 at 25.346 kb on - strand, within GFF2899 at 25.358 kb on - strand, within GFF2899 at 25.484 kb on + strand, within GFF2899 at 25.568 kb on - strand at 25.635 kb on + strand at 25.636 kb on - strand at 25.749 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction LB_plus_SM_buffer with Ffm_phage 0.001875 MOI remove 23,321 - GFF2896 0.67 +0.3 23,382 - GFF2896 0.71 -0.0 23,508 + GFF2896 0.79 -0.2 23,509 - GFF2896 0.79 -0.1 23,620 + GFF2896 0.87 +1.4 23,627 + GFF2896 0.87 -0.1 23,760 - +0.2 23,795 + +0.0 23,796 - +0.4 23,799 - +0.0 23,803 + +0.4 23,804 - +0.3 23,822 - +0.6 23,822 - -0.6 23,848 - -1.2 23,869 + +0.3 23,893 - -0.1 23,893 - +0.6 23,947 + +0.4 24,258 + GFF2897 0.32 +0.1 24,283 + GFF2897 0.34 +1.0 24,635 - GFF2897 0.74 +0.2 24,876 + +0.7 24,915 - GFF2898 0.14 +0.5 24,987 - GFF2898 0.35 +0.4 24,987 - GFF2898 0.35 -0.6 24,999 - GFF2898 0.39 +0.7 25,056 + GFF2898 0.55 -0.5 25,057 - GFF2898 0.56 -0.2 25,057 - GFF2898 0.56 -0.4 25,130 + GFF2898 0.77 -0.8 25,240 + GFF2899 0.11 -0.0 25,304 - GFF2899 0.31 -0.1 25,341 + GFF2899 0.43 +0.4 25,341 + GFF2899 0.43 -1.3 25,342 - GFF2899 0.43 +0.1 25,342 - GFF2899 0.43 -0.5 25,346 - GFF2899 0.44 +0.2 25,358 - GFF2899 0.48 +0.2 25,484 + GFF2899 0.88 +0.3 25,568 - -0.3 25,635 + +0.3 25,636 - -0.1 25,749 + +0.5
Or see this region's nucleotide sequence